Ukuhlaziywa kokudla nokuphuza isici esibalulekile sokuqinisekisa ukuphepha komkhiqizo, ikhwalithi, kanye nokuhambisana namazinga alawulayo. Elinye lamathuluzi abalulekile kule nqubo isihlungi sesirinji.Izihlungi zesirinjidlala indima ebalulekile ukulungiswa kwesampula ngokususa izinhlayiya kanye nokungcola okungaphazamisa imiphumela yokuhlaziya. Kodwa-ke, ngezinhlobo ezahlukahlukene zokukhethwa emakethe, ukhetha isihlungi esifanele sesirinji sokuhlaziywa kokudla nokuhlaziywa kwesiphuzo kungaba ngumsebenzi oyinselele. Lo mbhalo uxoxa ngezinto ezibalulekile okufanele ucabangele lapho ukhetha isihlungi esirinji ngezidingo zakho ezithile zokuhlaziya.

1. Ukuhlunga okubonakalayo

Nylon: Izihlungi zesirinji zeNylonbaziwa ngokusebenza kwabo ngokuhlukahlukene kanye nokuhambisana nezinhlobo eziningi zezinhlobo zesampula. Ukuqina kwabo kanye nekhono lokumelana namazinga okushisa alinganiselayo kanye nezingcindezi zibenze balungele imisebenzi yokuhlunga ejwayelekile ekudleni nasezifutho zokuhlaziya iziphuzo. Ukuhlunga kwe-nylon kuvame ukusetshenziselwa ukucacisa amasampula, ususe ama-concleates, futhi ulungiselele amasampula nge-HPLC (Phezulu Phezulu i-Chromatography) Ukuhlaziywa.

I-Polytetrafluoroethylene (PTFE): Izihlungi ze-PTFEIba nokuphikiswa okuhle kwamakhemikhali futhi ilungele amasampula anolaka aqukethe izixazululo ze-organic, ama-acid, noma izisekelo. Bayanene futhi bangasebenzi, banciphisa ukuphazanyiswa nge-analyte ngesikhathi sokuhlunga. Izihlungi ze-PTFE zivame ukusetshenziswa ukuhlaziywa kwe-LIPID, ukuhlaziya okuguquguqukayo okuguquguqukayo, kanye nezinhlelo zokusebenza ezidinga ubumsulwa obuphezulu kanye nokukhishwa okuphansi.

Polyethersulfone (PES):Izihlungi ze-Pes zihlanganisa ukumelana kahle kwamakhemikhali ngamanani aphezulu okugeleza futhi alungele izixazululo zamanzi, uketshezi lomzimba, namasampula abucayi. Imvamisa isetshenziswa ezindaweni zokugcina ze-microbiology zokuhlunga okuthe xaxa kwabezindaba, ama-buffers, namasampula esiko.Izihlungi ze-PesZiyahambisana futhi nezixazululo zamaprotheni futhi zingasetshenziselwa ukuhlunga kwamaprotheni kanye nokuhlaziywa kwamasampula okudla.

Cellulose acetate: Izihlungi ze-Cellulose Acetateyi-hydrophilic futhi iyahambisana namasampula asuselwa emanzini. Zivame ukusetshenziselwa ukucacisa iziphuzo ezifana nejusi, iwayini, nemikhiqizo yobisi. Izihlungi ze-CelLelose Acetate zisebenza ngokususa izinhlayiya, i-sediment, kanye ne-colloidal material, ukuqinisekisa isampula ecacile, eyizinhlayiya mahhala ukuze ihlaziywe.

Thola ukuthi yisiphi isihlungi se-syrinji, i-PVDF noma i-nylon, silungele kangcono izidingo zakho kule ndaba efundwayo. Thola imininingwane yokwenza isinqumo esinolwazi sezidingo zakho zokuhlunga:Izihlungi zesirinji ze-PVDF vs.

2 usayizi wobukhulu

0.2 micron pore usayizi:Izihlungi ezineUsayizi we-Pore of 0.2 micronzithathwa njengezinyumba futhi zisetshenziselwa kabanzi ukuhlaziya ama-microbial kanye nokufakwa kwesampula. Basusa ngempumelelo amagciwane, imvubelo, isikhunta, namanye ama-microorganisms, baqinisekisa amasampula aphephile aphephile wokuhlolwa. Izihlungi ze-micron zibalulekile ekulawulweni kwekhwalithi ekudleni nasezimboni zephuzo ukuvikela ukungcoliswa kwe-microbial futhi kuqinisekiswe ukuphepha komkhiqizo.

Uma ufuna ukwazi izihlungi ze-micron ezi-0,22, ungaphuthelwa le ndatshana. Thola imininingwane nolwazi mayelana nalawa mathuluzi abalulekile we-fieltration:Igayidi ephelele yokuhlunga okungu-0.22 micron: konke okudingeka ukwazi

0.45 micro pore usayizi: 0.45 micron pore usayizi izihlungiZilungele izinhlelo zokusebenza ezidinga ukususwa kwezinhlayiya ezinkulu, amaprotheni, kanye nemfucumfucu yeselula. Bajwayele ukusetshenziselwa ukuhlaziya amaprotheni, ukucaciswa kwesampula, kanye nokuqanjwa kwangaphambi kwezinyathelo ezinhle kakhulu zokuhlunga. I-0.45 fitter FIANCREL FIANTRAME FILFRACE EFFINACE nge-Flow Rate, Ubenze Baguquguquko Ngezinqubo Ezihlukahlukene Zokuhlaziya.

Hlola konke okudingeka ukwazi ngezihlungi ze-micron eziyi-0,45 kule ndaba efundwayo. Thola imininingwane ebalulekile futhi wenze izinqumo ezinolwazi mayelana nezidingo zakho zokuhlunga:Igayidi ephelele yokuhlunga okungu-0.45 micron: konke okudingeka ukwazi

3. I-hydrophilic ne-hydrophobic

Izihlungi ze-Hydrophilic:IHydrophilic Syringe izihlungi zenzelwe ukuheha ama-molekyuli wamanzi, okwenza lula ukumanzisa ngokushesha kanye nokuhlunga kwamasampula anamanzi. Bavimbela ukugcinwa kwamanzi ku-membrane yokuhlunga, ukunciphisa ukulahleka kwesampula kanye nokwenza ngcono ukufozela ukusebenza kahle. Izihlungi ze-Hydrophilic zilungele amasampula asuselwa emanzini, ama-buffers, uketshezi lomzimba, kanye nezixazululo zamanzi asetshenziswa kakhulu ekuhlaziyweni kokudla nokuhlaziywa kwesiphuzo.

Izihlungi ze-Hydrophobic:IHydrophobic Syringe Phindrs iyaphindisela amanzi futhi yenzelwe amasampula angewona anamanzi, izixazululo ze-organic, amafutha namasampula aqukethe ama-lipid. Bavimbela ukumunca isampula ku-membrane yokuhlunga, gcina ubuqotho bezinkomputha, bese uvikela ukuvalwa kwesihlungi. Ukuhlunga kwe-Hydrophobic kubalulekile ukuze kusetshenziswe izicelo ezinjengokuhlaziywa kwamafutha, ukulungiswa kwe-solvent, kanye nokulungiswa kwesampula kwegesi i-chromatography (GC) noma ukuhlaziywa kwe-mass spectrometry (i-MS).

4. Ukuqina

Izihlungi ezinyumba ezingenagciwane:Izihlungi zesirinji ezihambisanayo ze-Gamma ezishaywayo zifakwa inzalo ngokuchayeka emisebeni ye-gamma. Le nqubo isusa ngempumelelo ama-microorganisms afana namagciwane, amagciwane, kanye nesikhunta, uqinisekise ukuhlunga okungumuntu okuthe xaxa kwesampula. Izihlungi ze-Gammarradiation zibalulekile ekuhlolweni kwe-microbiological, imisebenzi yama-cell Culture, kanye nezinhlelo zokusebenza ezidinga izimo eziyinyumba ukuvikela ukungcoliswa nokugcina ubumsulwa besampula.

Izihlungi ezi-Autoclaved:I-Autoclaving enye indlela evamile esetshenziselwa ukuqinisa izihlungi zesirinji ngokuziveza kumusi oshisayo, ophakeme wokucindezela. Izihlungi ze-Autoclave Sterilization zilungele izinto ezingamelana ukushisa futhi zinikeze inzalo ethembekile yamasampula abucayi kanye noketshezi lomzimba. Zisetshenziswa kabanzi emathafeni e-microbiology, ukukhiqiza amakhambi, nokuhlolwa kokuphepha kokudla.

5. Ukuvumelana

Cabanga ngokuhambisana kokuhlunga kwesirinji nge-matrix ethile yesampula, i-solvent, amakhemikhali, kanye nokuhlaziywa kwenzalo.

Ukuvumelana kwamakhemikhali:Qinisekisa ukuthiokokuhlunga okubonakalayoIhambisana namakhemikhali nesampula kanye ne-reagents esetshenziswe ekuhlaziyweni. Qinisekisa ukuhambisana nama-acid, izisekelo, izixazululo ze-organic, namakhemikhali anokhahlo ukuvimbela ukuhlunga ukonakala, ukufuya, noma ukuphazamiseka ngemiphumela yokuhlaziya.

Isampula Matrix:Ama-matric ahlukile wesampula angadinga izinto ezikhethekile zokuhlunga kanye nosayizi we-pore ukufezekisa ukuhlunga okuhle nokulungiswa kwesampula. Cabanga ngokubonakala, ph, lokushisa, nokwakheka kwe-matrix yesampula lapho ukhetha izihlungi zesirinji ukuqinisekisa imiphumela esebenza kahle nethembekile.

Ukuqina kokuhlaziya:Okokuhlaziywa okubucayi, khetha izihlungi ezinama-extractable aphansi kanye nokuphazanyiswa okuncane ukuze ulondoloze ukuqina nobuqotho ngesikhathi sokuhlunga. Gwema ukusebenzisa izihlungi eziqukethe izinto eziboshwe ngamaprotheni noma ezinolaka ezingathinta ukunemba nokunemba kwezilinganiso zokuhlaziya.

Funda mayelana nokuthi kungenzeka ukusebenzisa izihlungi zesirinji kule ndatshana. Thola ukuthi izihlungi zesirinji zingasetshenziswa ngempumelelo izidingo zakho zokuhlunga:Ukuhlunga ngezimpawu uzokusebenzisa futhi?

6. Izinga lokugeleza

Ukuhlunga Okuphezulu Kwezinga:Izihlungi zesirinji ezinamanani aphezulu okugeleza anenzuzo ukucubungula amanani amakhulu amasampula ngokushesha nangendlela efanele. Banciphisa isikhathi sokuhlunga, bandise umkhiqizo welabhoratri, futhi baqinise ukucubungula okufika ngesikhathi kwamasampula. Kodwa-ke, ukunakekelwa kumele kuthathwe ukuze ulinganise izinga lokugeleza kanye nokusebenza kwe-filltration ukugwema ukuphumelela kwesampula, ukuhlunga ukuvalwa kwesampula, noma ikhwalithi yokuhlunga kabi.

Ukuhlunga okwenziwe kahle:Cabanga izihlungi ezenzelwe ukuletha izici ze-Flow ezenziwe kahle njengendawo yokulwa ne-membrane, ukusatshalaliswa komthengisi, nobukhulu be-membrane. Lezi zici zingathuthukisa ukusebenza kwe-fieltration, zinciphise i-backpressure, futhi zilondoloze amazinga okugeleza angaguquki ngaphezulu kwemijikelezo yokuhlunga eminingi.

7. Brand nekhwalithi

Khetha isihlungi sesirinji esivela kuhlobo oluthembekile olunerekhodi lethrekhi elifakazelwe lekhwalithi, ukuthembeka, kanye nokuhambisana namazinga ezimboni.

Imikhiqizo ongayethemba:Khetha izihlungi zesirinji kusuka kumikhiqizo edumile ngerekhodi elifakazelwe lekhwalithi, ukuthembeka, kanye nokuhambisana namazinga ezimboni. Imikhiqizo edumile yokutshala imali ku-R & D, izinqubo zokulawula ezisezingeni, kanye nezifundo zokuqinisekisa ukuqinisekisa ukusebenza okungaguquki nokwaneliseka kwamakhasimende.

Ukuqinisekisa ikhwalithi:Funa izihlungi zesirinji ezenzelwe izakhiwo eziqinisekiswe ze-ISO nge-UProtocols yekhwalithi eqinile yokuqinisekisa. Ukuhlunga okuphezulu kwekhwalithi ephezulu kuhlolwe i-batch, ukuhlolwa kobuqotho, nokuqinisekiswa ukuze uhlangane nokucaciswa kokusebenza, izidingo zokulawula, kanye nokulindelwe kwamakhasimende.

Ukungaguquguquki nokwethenjwa:QokaIzihlungi zesirinjiLokho kuhlinzeka ngokusebenza okungaguquki kwe-Filtration, ukuhluka okuncane kwe-batch-to-batch, nemiphumela ethembekile. Ukusebenza kwe-fingervice okungaguquki kubalulekile ukuze kufelwe kabusha, ukuqinisekiswa kwendlela, kanye nobuqotho bedatha ekuhlaziyweni kokudla kanye nokuhlaziywa kwesiphuzo.

Izihlungi ezi-8

Izihlungi zokuhlaziywa kwamaphrotheni:Khetha izihlungi zesirinji ezinezakhiwo eziphansi ezibophayo ukuvikela amaprotheni adsorption, ukulahleka kwesampula, nokuphazamiseka ngokuhlaziywa kwamaprotheni. Lezi zihlungi zenzelwe ukugcina amaprotheni ngenkathi zivumela ezinye izakhi ukuthi zidlule, ziqinisekise inani elinembile kanye nohlobo lwamaprotheni kumasampula okudla.

Izihlungi zokuhlaziywa kwe-Lipid:Khetha izihlungi ezimelana nama-lipid, ama-oyela, nezinto ezinamafutha ukuvikela ukungcoliswa kokuhlunga, ukungcoliswa kwesampula, noma amaphutha wokuhlaziya ekuhlaziyweni kwe-LIPID. Ukuhlunga okuhambisanayo kwe-Lipid kugcina ukusebenza kahle kwe-FILTRATION NOBUCHWEPHESHE ngesikhathi sokukhishwa kwe-LIPID, ukuhlanzwa, kanye nenqubo yokuhlaziya.

Izihlungi zokuhlaziywa okuguquguqukayoSebenzisa izihlungi zesirinji eziklanyelwe ukugcina amakhompiyutha aguqukayo kugesi ye-chromatography (GC) noma ukuhlaziywa kwama-spectrometry (MS). Lezi zihlungi zivimbela ukulahleka kokuhlaziywa okuguquguqukayo, kuthuthukise ukusebenza kahle kokuhlukanisa, futhi kwandise ukuzwela kokutholwa kwamakhompiyutha ajwayelekile ezinto ezi-organic (ama-Vocs), amakhompiyutha amnandi ekudleni nasezisikeni zamasampula.

Ukucabanga ngalezi zinto ezinemininingwane nokukhetha okulungileisihlungi sesirinjiNgokusekelwe ekudleni okukhethekile kokudla nezidingo zakho zokuhlaziya kungahle kulungiswe ukulungiswa kwesampula, ukuthuthukisa ukusebenza kwabahlaziywe, futhi kuqinisekiswe imiphumela enembile nethembekile ekuhambeni komsebenzi kwelabhoratri wakho.

Vula izimpendulo zemibuzo engama-50 ejwayelekile mayelana nokuhlunga ngezimpawu kule ndaba efundwayo. Dlulisa emhlabeni wokuhlunga kwesirinji futhi uthole izixazululo zemibuzo yakho:Isihloko se- "Syringe Filter" Imibuzo Ebuzwa Njalo Kaningi

Okwedlule:

Ukuhambisana nezinto zokwakha ezisetshenziswa ku-Chromatogragraphy Vavials ngezokuphepha ezenzakade kanye namazinga okuhlanzana

Olandelayo:

Ungasigcina kanjani kahle futhi usiphathe kahle ama-chromatography vials