I-HeadSpace Gagesi Chromatography (HS-GC) inqubo enamandla yokuhlaziya yokuhlaziya yokuhlaziya amakhompiyutha aguqukayo kumatikuletsheni ayisampula ahlukahlukene, kufaka phakathi uketshezi nama-solids. Indlela ekhipha kahle ukuhlaziya okuguqukayo kusuka kumatric ayinkimbinkimbi ngaphandle kokufaka isampula lonke ngqo ku-chromatograph (GC). Esikhundleni salokho, i-HS-GC ihlaziya isigaba segesi ngenhla kwesampula, eyaziwa ngokuthi yi-headpace. Le bhulogi izomboza konke okudingeka ukwazi ngokulungiselela amasampula e-HeadSpace GC, kufaka phakathi izimiso zayo, amasu, izinzuzo, kanye nemikhuba emihle kakhulu.

Ufuna ukwazi ngokugcwele nge-infor mayelana ne-Headspace Vial, sicela uhlole le ndatshana: Umhlahlandlela ophelele wezinhloko ze-Headspace: Izici, Ukukhetha, intengo, nokusebenzisa

Ukuqonda isampula le-headpace

ISampling ye-HeadSpace isuselwe kwisimiso sokuthi amakhompiyutha aguqukayo esampuli angahlukaniswa esigabeni segesi ngenhla kwesampula lapho kufakwa. Indlela ewusizo ikakhulukazi ekuhlaziyeni amakhompiyutha e-turatile organic (ama-Vocs), izixazululo eziseleyo, nezinye izinto ezibi kakhulu. Izindlela ze-HeadSpace zinciphisa ukwethulwa kwezinto ezingezona eziguqukayo kanye nokungcola ohlelweni lwe-GC, okuholela ekuhlanzeni ama-chromatograms kanye nemiphumela ethembekile.

Imiqondo ebalulekile yeSampling ye-HeadSpace

Ukulingana:Lapho kufakwa isampula ngaphakathi isivalo esivaliwe Futhi ukufudumala, amakhompiyutha aguqukayo azohamba asuka esigabeni esiwuketshezi noma esiqinile esigabeni segesi kuze kube yilapho ukulingana kufinyelelwa. Ukuqoqwa kwe-analyte ekhanda kuncike ekunciphiseni kwayo kanye nokushisa kwesampula.

Ukuhlukaniswa Okuhlanganisiwe (k):I-coeffrent ehlukanisayo iyisici esibalulekile kwisampula ye-headpace. Kuchaza ukusatshalaliswa kwamakhompiyutha aguqukayo phakathi kwezigaba eziwuketshezi negesi. Amakhompiyutha anamanani aphansi k ajwayele ukwahlukanisa kalula esigabeni segesi ngakho-ke atholakele kalula.

I-Static VS. Dynamic Headspace:ISampling ye-HeadSpace ingenziwa kusetshenziswa izindlela ezi-static noma ezinamandla. I-Static Headspace ifaka ukuvumela isampula ukufinyelela ukulingana endaweni ebekwe uphawu, ngenkathi i-headpace eshukumisayo ifaka ngokuhlanza isampula nge-inert ground ukushanela amakhompiyutha eguquguqukayo esigabeni segesi.

I-HeadSpace Gas Chromatography Suply Fasket

Ukulungiswa kwesampula okufanele kubalulekile ekutholeni imiphumela enembile nezezimali ekuhlaziyweni kwe-HeadSpace Gas Chromatography. Izinyathelo ezilandelayo zichaza ukucatshangelwa okuyisisekelo kokulungiselela amasampula ukuhlaziya kwe-headSpace.

1. Khetha isampula efanelekile

Ukukhetha kwesokudlaisampula vialibalulekile kwisampula ye-headpace ephumelelayo. Amasayizi ajwayelekile we-vial afaka i-6ml, 10ml, no-20ml, ngeI-20ml vialsUkuba okuvame ukusetshenziswa. Izici ezibalulekile okufanele ucabangele lapho ukhetha ama-vials kufaka phakathi:

Okubonakalayo:Ama-vials ngokujwayelekile enziwe ngengilazi noma ngeplastiki.Izingilazi zeglasizilungele kangcono amasampula aguqukayo ngenxa yokuvuselelwa kwawo kanye namathuba aphansi wokungcola okulimazayo.

I-Seating Mechanism:Ama-vials angabekwa uphawu nge-crimp noma i-screw cap. Crimp vialsNikeza uphawu lwe-Airtight, olubaluleke kakhulu ukugcina ubuqotho be-headpace.

Ikhwalithi ye-Septum:I-Septa esetshenziselwa ukubopha ama-vials ingakwazi ukwethula ukungcola uma ingekhwalithi engeyinhle. Funa nge-Septa eyenzelwe ngqo izinhlelo zokusebenza ze-Headspace, ngoba mancane amathuba okuba afake i-headpace.

2. Isampula yevolumu ne-dilution

Ivolumu yesampula ku-vial ibalulekile ekufezeni ukugxila okuhle kwe-headspace. Ngokuvamile, ivolumu yesampuli kufanele libe cishe ngo-1 \ 3 kuya ku-1 \ 2 kwevolumu eliphelele le-vial ukuhlinzeka ngekhanda elanele lesigaba segesi.

Dilution:Uma ukugxila kwesampula kuphezulu kakhulu, kungaholela ekutholeni i-headpace egcwele, okuholela ekunciphiseni okungalungile. Ukunciphisa isampula nge-solvent efanele kungasiza ukufeza ukugcwala okufunwa kokuhlaziywa okuguqukayo.

Ufuna ukwazi kabanzi ukuthi kungani ama-headpace vials asetshenziswa ku-chromatography ?, Sicela ubheke le Artice: Kungani ama-headspace vials asetshenziswa ku-chromatography? 12 engles

3. Ukulawulwa kokushisa

Ukushisa kudlala indima ebalulekile kwisampula ye-headpace njengoba kuthinta ukuguquguquka kokuhlaziywa kokuhlaziya nokuhlukaniswa kwazo esigabeni segesi.

Izinga lokulinganisa lokulingana:Ama-vials wesampula kufanele afudunyezwe emazingeni okushisa alawulwayo ukukhuthaza ukukhishwa kwamakhompiyutha aguqukayo e-headpace. Izinga lokushisa elifanele lincike ekuhlaziyweni okuthile okuhlaziywa futhi kufanele kunqunywe ngesikhathi sokuthuthukiswa kwendlela.

Isikhathi sokulingana:Vumela isikhathi esanele sokuthola isampula ukufinyelela ukulingana. Lokhu kungahluka ngokuya nge-matrix eyisampula kanye nokuguquguquka kwamakhompiyutha. Izikhathi zokulingana ezijwayelekile zisukela emizuzwini engama-30 kuya emahoreni ambalwa.

4. Nciphisa ukungcola

Ukungcola kungakuthinta kakhulu ukunemba kwe-Headspace Gas Chromatography. Ukuze unciphise ubungozi bokungcola, yenza okulandelayo:

Sebenzisa izipesheli zangaphambi kokuhlanzwa:Ngaso sonke isikhathi sebenzisa ama-vials ahlanjululwe ngaphambi kokugwema ukwethulwa kokungcola ngesikhathi sokupakisha noma ukuphathwa.

Izikhala ezingenalutho:Run indlela izikhala ukukhomba imithombo engaba khona yokungcola. Lokhu kufaka ukuhlaziya isampula elingenalutho usebenzisa ukulungisa okufanayo kanye nezinqubo zokuhlaziya ukuqinisekisa ukuthi azikho iziqongo ezingafuneki zivela ku-chromatograph.

Izimo ezilawulwayo zemvelo:Yenza ukulungiswa kwesampuli endaweni ehlanzekile ukunciphisa ukuvezwa kwezinto ezingcolisayo zezindiza.

5. Khetha inqubo efanelekile ye-HeadSpace

Njengoba kushiwo ngaphambili, isampula ye-headpace ingaba yi-static noma inamandla. Ukukhethwa kwenqubo kuya ngohlelo oluthile kanye nohlobo lwesampula.

I-Static Headpace:Le ndlela ilungele izinhlelo eziningi futhi isetshenziswa kabanzi ukuhlaziya amakhompiyutha aguqukayo ku-liquids nama-solids. Ivumela ukuhlaziya ukuthi ukwahlukanisa ngokwemvelo ku-Headspace ngaphandle kokwethulwa kwegesi eyengeziwe.

I-Dynamic Headpace:Le ndlela ilungele kangcono amasampula adinga ukuhlanza okuqhubekayo ukuze athumbe amakhompiyutha aguqukayo. Imvamisa isetshenziswa kuzinhlelo zokusebenza ezinjengokuhlolwa kwemvelo nokuhlaziywa kokudla lapho amasampula angaqukatha ukugxila okuphansi kokuhlaziywa.

Izinzuzo ze-Headspace GC Sampling

Ama-Chromather Chromatograms:Ngokuhlaziya kuphela isigaba se-vapor, isampula ye-headpace inciphisa ukwethulwa kwezinto ezingaguquki kanye nokungcola, okuholela ekuhlanzeni ama-chromatograms kanye nokusebenza okuhlaziya okuthuthukile.

Isikhathi sokulungiselela isampula:I-Headspace Sampling yenza lula inqubo yokulungiselela isampula, ngoba isusa isidingo sezinqubo eziyinkimbinkimbi zokukhishwa noma izinqubo zokuthola usizo.

Ukwanda kokuzwela:Ukuhlaziywa kokuhlaziywa okuguquguqukayo e-Headspace kungaholela ekukeni okuthuthukayo, okwenza kube lula ukuthola amakhompiyutha aqanjiwe.

Okuhlukahlukene:I-HeadSpace Sampling ingafakwa ezinhlotsheni eziningi zesampula, kufaka phakathi uketshezi, ama-solids, kanye namatikuleshini ayinkimbinkimbi, okwenza kube yindlela eguquguqukayo yezinhlelo zokusebenza ezahlukahlukene.

Ufuna ukwazi kabanzi ngokulinganisa kwengcindezi kwama-vials ase-Aijiren Headspace nama-CAPS, sicela ubheke le Artice: Kuyini ukulinganisa kwengcindezi kwama-vials ase-Aijiren headspace nezigqoko?

Ukugcina

I-HeadSpace Gagesi Chromatographykuyindlela ebalulekile yokuhlaziya yokuhlaziywa kwamakhompiyutha aguqukayo kumatikuletsheni ahlukahlukene wesampula. Ukulungiswa kwesampula okufanele kubalulekile ukuze kufinyelelwe imiphumela enembile nezenzekayo ekuhlaziyweni kwe-HeadSpace GC. Ngokuqonda imigomo ye-HeadSpace Sampling futhi ulandela izindlela ezingcono kakhulu zokulungiswa kwesampula, ama-laboratories angathuthukisa ukusebenza kahle nokwethembeka kokuhlaziywa kwawo. Njengoba isidingo samasu wokuhlaziya asezingeni eliphakeme siyaqhubeka nokukhula, i-headpace GC Sampling izohlala iyithuluzi elibalulekile labacwaningi kanye nabahlaziyi bezinkomo eziningi.

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