I-HPLC vs. LCMS: Yikuphi okufanele ukhethe? Amaphuzu ama-5

Umgomo Wokusebenza

I-HPLC

I-HPLC yindlela ye-Chromatographic ehlukanisa amakhompiyutha asuselwa ekusebenzisaneni kwawo ngezigaba ezimile neziselula. Ku-HPLC, isigaba seselula esiwuketshezi siphonswa kwikholamu esigcwele isigaba esimile, esivame ukwakhiwa ngezinhlayiya ezincane zeSilica noma ama-polymeter. Lapho ingxubevange yesampula yethulwa ohlelweni, izakhi zihlukaniswe ngokususelwa ekubunyeni kwabo esigabeni seminyaka. Amakhompiyutha asebenzisana kakhulu ngesigaba esimisiwe esisezingeni eliphansi kancane kancane, kuyilapho ama-compound asebenzisana kancane kakhulu. Amakhompiyutha ahlukanisiwe abese ethola kusetshenziswa abakwa-Ehlukahlukene abatholayo, njenge-UV-Vis, i-Fluorescence, noma i-Detectors yenkomba yenkomba.

Ufuna ukwazi kabanzi mayelana nokusetshenziswa kwama-chromatography vials, sicela ubheke le ndatshana:Izicelo eziyi-15 zesimo se-Chromatography emikhakheni ehlukene

Lcms

I-LCMS ihlanganisa amakhono ahlukanisayo e-HPLC ngamakhono wokuhlaziya ngobuningi be-Mass Spectrometry. Ku-LCMS, amasampula ahlukaniswe kuqala kusetshenziswa i-HPLC, njengase-HPLC ejwayelekile. Kodwa-ke, esikhundleni sokutholwa ngumtshina ojwayelekile, amakhompiyutha afakiwe angeniswa ku-Spectrometer. I-Mass Spectrometer ionizes amakhompiyutha futhi ilinganise isilinganiso sayo sokushayisana, ukuhlinzeka ngemininingwane enemininingwane ngesisindo se-molecular kanye nokwakheka kwe-analyte. Le nhlanganisela yokuhlukanisa kanye ne-mass spectrometry inikeza ukuzwela okukhulu nokucaciswa kune-HPLC yedwa.

Indlela yokuhlukanisa

I-HPLC:I-HPLC isebenzisa ingcindezi ephezulu yokucindezela isigaba seselula esiwuketshezi ngekholomu egcwele equkethe isigaba seminyaka. Amakhompiyutha ahlukaniswe ngokuya ngokuxhumana kwawo okuhlukile nesigaba sokuma, okuholela ekuhlukaniseni okunembile nokuluyo.

I-LCMS:I-LCMS ihlanganisa amakhono okuhlukanisa ngokomzimba we-HPLC ngamakhono amaningi we-mass spectrometry we-Mass Spectrometry (MS). Ingxenye ye-chromatography ye-chromatography ihlukanisa amakhompiyutha, ngenkathi i-mass spectrometer ilinganisa isilinganiso se-mass-to-Chirt of the ionized species.

Ukuzwela nokukhetha

I-HPLC:I-HPLC inokuzwela okuhle kanye nokukhetha, ikakhulukazi lapho kuhlanganiswe nokuthola okuthuthukile okufana ne-UV-vis, i-fluorescence, noma i-diode array detectors. Kodwa-ke, i-HPLC yedwa kungenzeka ingahlinzeki ngokucaciswa okwanele kwamasampula ayinkimbinkimbi.

I-LCMS:I-LCMS inokuzwela okuphakeme kanye nokukhetha okuqhathaniswa ne-HPLC. Ama-spectrometers amaningi angakhomba ngokunembile futhi anciphise amakhompiyutha asuselwa kumakati abo ahlukile wokushaja, ngisho nakumatikuletsheni okuyinkimbinkimbi. Lokhu kwenza i-LCMS ilusizo ikakhulukazi ekuhlaziyeni izinhlanganisela zokulandela futhi iqinisekise ubunikazi bezinto ezingaziwa.

I-HPLC:Ukulungiswa kwesampuli kwe-HPLC ngokuvamile kubandakanya ukujabula, ukufombula, noma amasu alula wokukhipha. Amasampula awadingi ukuhwanyelwa, ngakho-ke i-HPLC ilungele amakhompiyutha ahlukahlukene, kufaka phakathi izinto zePolar nezengezona ezingezona zodwa.

I-LCMS:Ukulungiswa kwesampuli ye-LCMS kuyafana nalokho kwe-HPLC, kepha izinyathelo ezengeziwe zingacelwa ukuthi ziqinisekise ukuhambisana ne-Mass Spectrometer. Isibonelo, ama-buffers aguqukayo noma izengezo kungadingeka ukuthuthukisa i-ionization futhi uvikele ukucindezelwa kwe-Ion.

Izinja

I-HPLC:I-HPLC isetshenziswa kabanzi ekuhlaziyweni kwemithi, ukudla kanye namasampula emvelo. Isebenza ngokukhethekile ukuhlukanisa nokuhlukanisa amakhompiyutha ekuxubeni okuyinkimbinkimbi, njengokungcola kwezidakamizwa, izengezo zokudla, nezinsalela zokubulala izinambuzane.

I-LCMS:I-LCMS inezinhlobonhlobo zezicelo, kufaka phakathi:

Ukuhlaziywa Kwemithi: Kusetshenziswe ekutholweni kwezidakamizwa, ukuthuthukiswa, kanye nokulawulwa kwekhwalithi.

Amaprotheni kanye ne-metabolomics: Kusetshenziselwa ukukhomba kanye nokunciphisa amaprotheni nama-metabolites kumasampula endalo.

Ukuhlaziywa Kwezemvelo: Kusetshenziselwa ukuthola ukulandelela ukungcola emanzini, inhlabathi namasampula e-air.

Ufuna ukwazi ngendima yama-vials aqinisekisiwe we-HPLC ekuqinisekiseni ukuhlaziya okunembile kwesampula? Funda le ndatshana: Ukubaluleka kwama-HPLC Vavials aqinisekisiwe wokuhlaziywa kwesampula okunembile

Ukugcina

I-HPLC ne-LCMS kungamasu wokuhlaziya anamandla ngamandla abo kanye nemikhawulo yabo. Ukuzikhethela phakathi kwalokhu okubili kuncike kwizidingo ezithile zokuhlaziywa, njengokuzwela, ukukhetha, ubunzima obuyinkimbinkimbi, nezinsizakusebenza ezikhona.

I-HPLC iyisinqumo esihle sokuhlaziywa okujwayelekile kwamakhompiyutha owaziwayo kumatikuletsheni olula, lapho izindleko nokusebenziseka kalula kuyizinto ezibalulekile. Ngakolunye uhlangothi, i-LCMS, yindlela ekhethwayo yokuhlaziya amasampula ayinkimbinkimbi, ekhomba amakhompiyutha angaziwa, futhi uzuze ukutholwa kwezinga le-Ultra-trace.

Njengoba ubuchwepheshe buqhubeka buqhubekisela phambili, ukuhlanganiswa kwe-HPLC kanye ne-LCMS kuzodlala indima ebaluleke kakhulu ekuhlangabezaneni nezidingo ezivelayo zamakhemikhali wokuhlaziya kanye nokuqinisekisa imiphumela esezingeni eliphakeme kuzo zonke izinhlelo zokusebenza ezinhlobonhlobo.