Izihlungi zesirinjiamathuluzi abalulekile kuzilungiselelo zelebhu, ikakhulukazi ekuqinisekiseni ubumsulwa besampula ngaphambi kokuhlaziywa. Enye yezinto ezibaluleke kakhulu ezithinta ukusebenza kwazo usayizi wobukhulu. Usayizi we-pore wesihlunga sesirinji uthinta ngqo amandla okususa okungokwemvelo, okuthinta ubumsulwa besampula. Lo mbhalo uhlola ukuthi usayizi we-pore uphendula kanjani ubumsulwa besampula nokuthi yini okufanele uyicabangele lapho ukhetha isihlungi esirinjisiwe.

Ukuqonda izihlungi zesirinji

Izihlungi zesirinji kumadivayisi we-fieltration alahlekayo angena ekugcineni kwesirinji. Ziqukethe ulwelwesi olunobukhulu obuthile be-pore futhi zisetshenziselwa ukususa izinhlayiya, ama-microorganisms, nezinye izinto ezingcolile kusuka kumasampula e-liquid ngaphambi kokuhlaziywa. Ukukhethwa kosayizi we-pore kubalulekile njengoba kunquma ukuthi yikuphi ukungcola okungahlungwa ngempumelelo.

Imibuzo ebuzwa njalo mayelana nokuhlunga ngezimpawu, qiniseka ukuthi ubheka le ndatshana efundisayo:Isihloko se- "Syringe Filter" Imibuzo Ebuzwa Njalo Kaningi

Umthelela ngosayizi wobumsulwa besampula

Ukusebenza kahle kokususa

Umsebenzi oyinhloko wokuhlunga kwesirinji ukususa izinhlayiya ezingafuneki kusuka kusampula. Usayizi we-pore unquma ukuthi izinhlayiya zingadlula kusihlungi:

Usayizi omncane we-pore (e.g. 0.22 μm): Lezi zihlungi zivame ukusetshenziselwa inzalo futhi zisebenza ekususeni amagciwane nezinhlayiya ezinkulu. Baqinisekisa ukuthi amasampula angenakungcoliswa kwe-microbial, okubucayi ocwaningweni lwe-microbiological kanye nezicelo zemithi.

Amasayizi amakhulu e-pore (e.g., 0.45 μm): Ngenkathi lezi zihlungi zingasusa izinhlayiya ezinkulu, kungenzeka zingabi nakancane ekuqedeni okungcola okuncane, njengamagciwane noma izinto ezithile ze-colloidal. Ngakho-ke, uma kungcola okuncane kukhona, kusetshenziswa usayizi omkhulu we-pore kungahlehlisa ubumsulwa besampula.

Inani lokugqwala nokugeleza

Ukukhethwa kosayizi we-pore kuthinta nokugeleza kwesihlungi kanye nokuthambekela kokuqothuka:

Osayizi abancane be-Pores: Ngenkathi zisebenza kahle ekuhlungeleni ezingcolisayo ezincane, zithambekele kakhulu ekugqokeni, ikakhulukazi lapho amasampula equkethe ukugxila okuphezulu kwezinhlayiya. Imiphumela Emiphumela Ekukhuliseni i-BackSpressure, engaholela ekuhlutjweni okuhamba kancane futhi ingahlehlisa ubuqotho besampula.

Amasayizi amakhulu e-Pores: Lezi zihlungi ngokuvamile zivumela amazinga okuhamba ngokushesha, anciphisa ubungozi bokuvalwa. Kodwa-ke, lokhu kuza ngokulahlekelwa kokuvumela ukungcoliswa okuncane ukuthi kudlule, ukuyekethisa ubumsulwa besampula.

Umthelela emiphumeleni yokuhlaziya

Ukuba khona kokungcola kungathinta kakhulu imiphumela yokuhlaziya. Isibonelo, ukungcola kungaphazamisa ukuhlaziywa kwe-chromatographic noma ama-mastecromeetry wokubuka, okuholela ekuhlolweni okungalungile noma ukukhonjwa:

Idatha enembile: Usebenzisa usayizi ofanele we-pore uqinisekisa ukuthi kuphela umhlaziyi onentshisekelo okhona kwisampula, okuholela emiphumeleni ethembekile futhi ephindekanze.

Ukulawulwa kwekhwalithi: Kuhlelo lokusebenza lwezemithi, ukulondolozela amazinga aphezulu wokuhlanzeka kubalulekile ekufezeni amazinga alawulayo. Usebenzisa usayizi ofanele we-pore kusiza ekuqinisekiseni ukuthi umkhiqizo wokugcina uhlangabezana namazinga okuhlanzeka adingekayo ngaphambi kokusatshalaliswa.

Ufuna ukwazi okuningi mayelana nezihlungi ze-micron eziyi-0,22, sicela uhlole le ndatshana:Igayidi ephelele yokuhlunga okungu-0.22 micron: konke okudingeka ukwazi

Ukukhetha usayizi ofanele we-pore

Lapho ukhetha isihlungi esirinji, kufanele kubhekwe izinto eziningana ukuqinisekisa ubumsulwa besampula ngokuphelele:

Uhlobo lwesampula: Kubalulekile ukuqonda ukuthi yikuphi ukungcola okudingeka kususwe. Ngokwesibonelo:

Uma isampula yemvelo ecutshungulwa icubungulwa ibalulekile ukuze isebenze, aIsihlungi se-0.22 μmkufanelekile.

Ngokuhlunga okujwayelekile lapho izinhlayiya ezinkulu kuphela ezidinga ukususwa, aIsihlungi se-0.45 μmkungenzeka kwanele.

IVolumu yesampula kanye nokuhlushwa: Ukugxila okuphezulu kwezinhlayiya kungadinga usayizi omncane we-pore ukuqinisekisa ukugcwala okusebenzayo ngaphandle kokuthinta izinga lokugeleza.

Ukuvumelana namasu wokuhlaziya: Izindlela ezahlukahlukene zokuhlaziya zingaba nezidingo ezithile zokuhlanzeka kwesampula:

Amasu anjenge-HPLC ngokuvamile adinga amasampula ahlanzekile kakhulu angenazinhlayiya ezingalimaza ikholamu noma umtshina.

Ukucatshangelwa Kwezindleko: Izihlungi ezinamasayizi amancane e-pore zibiza kakhulu ngenxa yezindleko zazo eziphakeme zokukhiqiza. Ukulinganisa izindleko ngezidingo zokusebenza kubalulekile kwizabelomali zelebhu.

Imikhuba emihle yokusebenzisa izihlungi zesirinji

Ukwandisa ubumsulwa besampula lapho usebenzisa izihlungi zesirinji:

Amasampula we-Preilter: Uma amasampula equkethe ukugxila okuphezulu kodaba lokubandakanya, cabanga ngokuzikhandla ngokusebenzisa isihlungi sosayizi omkhulu we-pore ngaphambi kokusebenzisa isihlungi sosayizi omncane we-pore.

Sebenzisa izihlungi ezintsha: Sebenzisa isihlungi esisha ngaso sonke isikhathi kwisampula ngayinye ukugwema ukungcoliswa.

Landela imihlahlandlela yomenzi: Namathela imiyalo yokusebenzisa enconyiwe maqondana nengcindezi enkulu nokuhambisana kwesampula ukuze ulondoloze ubuqotho bokuhlunga.

Ufuna ukwazi okuningi mayelana nokuhlunga okungu-0.45 micron, sicela ubheke le ndatshana:Igayidi ephelele yokuhlunga okungu-0.45 micron: konke okudingeka ukwazi

Ukugcina

Isihlungi se-Syringe Isihlungi se-Pore usayizi udlala indima ebalulekile ekutholeni ubumsulwa besampula ngokuthinta ukusebenza kahle kokususa, isilinganiso sokugeleza, nokunemba okuphelele kokuhlaziya. Ukukhetha usayizi ofanele we-pore kususelwa kuhlobo lwesampula kanye nokuhlaziywa okuhlosiwe kubalulekile ekufezekiseni imiphumela ethembekile.

Ngokuqonda ukuthi usayizi we-pore athinta kanjani ukusebenza kwe-filltration, abacwaningi bangenza izinqumo ezinolwazi lapho bekhethaIzihlungi zesirinji, Ekugcineni kwaqinisekisa idatha esezingeni eliphakeme kanye nokuhambisana namazinga okulawula ezinhlotsheni ezahlukahlukene ezivela kumakhamishini ekuqapheni kwezemvelo.

Okwedlule:

Yini umsebenzi wamashubhu wokuhlola ama-COD ekuhlaziyeni kwemvelo?

Olandelayo:

2 ml autosampler vials we-chromatography yegesi