Kuchita masewera olimbitsa thupi kwa ma chpomatography (hplc) kumagwiritsidwa ntchito kwambiri pochita kupatukana, kuzindikira, komanso kuchuluka kwa zinthu zosakaniza. Mwa owunika osiyanasiyana omwe amapezeka ku HPLC, UV-vis (UV-Vis-vis) ndizomwe zimapezeka kwambiri chifukwa chosavuta kugwiritsa ntchito, chidwi chachikulu, ndi kuthekera kopenda mitundu yosiyanasiyana. Nkhaniyi ikuwunika mitundu yosiyanasiyana ya oyesa a UV-IV, momwe amagwirira ntchito, mapindu ake, ndi malingaliro awo powasankha.

Mitundu ya Openda UV-Is

1. Woyambitsa ma uvi

Ofufuza owoneka amalola kuti miyeso imatengedwa pazinthu zina kapena zingapo. Amakonda kukhazikitsidwa mofatsa, monga 254 nm ya organic mankhwala. Zoyeserera izi ndizothandiza pakugwiritsa ntchito zomwe mukufuna.

Zoyeserera za PDA zimatha kuyeza spectra zingapo nthawi yomweyo, zomwe zimatha kusintha chidwi ndi kusanthula zomwe zimafunikira mavelo ambiri. Izi zimathandiza kuti zizindikiridwe bwino komanso kuchuluka kwa mankhwala kutengera mayamwidwe awo apadera.

3.. DIOD Ndondomeko (Abambo)

Zofanana ndi PDAS, abambo amalanda mawonekedwe athunthu pamlingo wamitundu ina munthawi yeniyeni. Izi ndizofunikira makamaka pamasamba ovuta pomwe mitundu ingapo imatha kukhala yosanja nthawi yomweyo. Abambo amatha kupereka zambiri zoyera komanso zothandiza kuzindikira zinthu zosadziwika kutengera ma prectal awo.

Mfundo Zachikhalidwe

Ofufuza a UV-vis amagwira ntchito podutsa UV ndi kuwala kowoneka bwino kudzera pa cell. Mwa kuyeza kuchuluka kwa kuwala komwe kumachitika ndi zitsanzo pamitundu yosiyanasiyana, chidwi ndi chizindikiritso chomwe chingamvekere.

Zigawo zazikuluzikulu:

Kuwala Kwakukulu: Nyali ya Deuteum imagwiritsidwa ntchito kuzindikiridwa kwa UV (190-400 nm) ndipo ingaphatikize nyali yankhanza kuti isaoneke (400-800 nm). Makina ena apamwamba amagwiritsa ntchito nyali za Xenon kuphimba magawo onse ochulukirapo kuti awonjezere mphamvu.

Kutulutsa kwa cell: Mapangidwe ndi luso la maselo otuluka ndizovuta kuti amveke bwino. Maselo ang'onoang'ono otayika amatha kusintha malire koma amatha kuyambitsa zovuta zowonjezera.

Cholemba: Cholembacho chimasinthiratu kusintha kwamphamvu kwambiri kukhala chizindikiro chamagetsi kukhala chizindikiro cholingana ndi kusanthula kwa chimbudzi.

Ndikufuna kudziwa zambiri za kukonzekera kwa HPLC, chonde onani nkhaniyi:Mayankho a HPLC Kukonzekera kwa Zotsatira Zabwino

Ubwino wa UV-Is Vis

Kuzindikira: Ofufuza a UV-vis-vis amatha kuyeza molondola kusintha molondola chifukwa chake amatha kuzindikira zosankha zochepa.

Zosintha: Zitha kugwiritsidwa ntchito m'mafashoni osiyanasiyana monga mankhwala opangira mankhwala, chakudya ndi zakumwa, kuyesedwa kwachilengedwe, ndi biochemin.

Kusanthula Kusanthula: Njirayi siyisintha kapena kuwononga chitsanzo, kuwunika kwina kumatha kuchitidwa ngati pakufunika.

Kuchulukana: Kutha kusanthula mitundu yambiri yokhala ndi ma chrokhophores (magulu owoneka bwino), oyenera kugwiritsa ntchito mitundu yosiyanasiyana.

Maganizo akasankha chojambula cha UV

1. Mitundu Yonse

Kusankha mitundu yosiyanasiyana ya magwero am'madzi (DeuteSten, natepsenten, ndi xenon) amakhudza mtundu womwe wafuumu womwe ungagwiritsidwe ntchito. Kuti muwunikidwe kwathunthu, makamaka kusanthula zosakaniza zovuta, mtundu wa maphalade umakonda.

2. Mtundu wa cholembera

Kusankha pakati pa cholembera ndi PDA \ / Abambo amatengera zosowa zenizeni:

Wotchinga: Zabwino kwambiri pakusanthula komwe kumapangitsa kuti kufalikira kwinakwake.

PDA \ / Abambo: Udiname ya zitsanzo zovuta zomwe zimafuna kusanthula kwa muyezo wambiri.

3. Kutulutsa kwa maselo

Mapangidwe amakhudza chidwi ndi chisankho:

Cell yochepa kwambiri yamagazi imathandizira kukhudzana.

Kutalika kwa njira ndikofunikanso; Kutalika kwa njira yayitali kumakulitsa miyeso yotsekemera koma yosangalatsa.

Kugwirizana ndi makina omwe alipo ndi HPLC ndi mawonekedwe ogwiritsa ntchito ogwiritsa ntchito amatha kusintha kwambiri labooktory.

Mapulogalamu a UV-Is Ipeni

Ofufuza a UV-Is

Mankhwala: Ntchito zowongolera bwino komanso kusanthula kosakanikirana.

Kuyesa kwachilengedwe: kuwunikira zodetsa nkhawa m'madzi.

Makampani Opanga Zakudya: Kusanthula Zowonjezera ndi Zodetsa.

Biotechnology: Kuyesa mapuloteni okhazikika mu zachilengedwe njira.

Mukufuna kudziwa zambiri za mapulogalamu a Mbiri ya Chromatography, chonde onani nkhaniyi:Mapulogalamu 15 a Chromatography Vials m'minda yosiyanasiyana

Kusankha chotchinga chabwino kwambiri cha HPLC cha UV-viss chimafuna kumvetsetsa zosowa zenizeni zomwe mungagwiritse ntchito, kuphatikiza zofuna zanzeru, zapamwamba, ndi zitsanzo. Pomwe ofufuza achikhalidwe amayenereradi kusanthula kosavuta, ma refordoc oyang'anira amapereka zabwino pamakhalidwe ndi kusinthasintha, ndikuwapangitsa kukhala oyenera ntchito zovuta kwambiri.

M'mbuyomu:

Mbale zopambana kwambiri kwa HPLC ndi GC Kusanthula: 5 mfundo

Ena:

Chilichonse chodziwa za zosefera zamapepala