Njira yoyendetsera madzi a ch commatography (HPLC) ndi njira yofunika yophunzitsira yomwe imagwiritsidwa ntchito m'minda yosiyanasiyana monga mankhwala, kusanthula kwachilengedwe, komanso chitetezo cha chakudya. Gawo lofunikira la HPLC System ndi SePtom, yomwe imangokhala yotseka vial. Septum ya vispom ndi kutsekedwa komwe kumasindikiza kutsegulidwa kwa vial, kulola singano singano ndikusunga chidindo chotetezeka kuti isadetse kuipitsidwa ndi kutuluka. Ayenera kukhala ogwirizana ndi zosungunulira kuwunikiridwa ndipo ayenera kupirira mikhalidwe ya HPLC.
Kufunika kosankha SePA yoyenera
Kusankha septum yoyenera ndikofunikira:
Kuletsa kuipitsidwa: Zinthu zosagwirizana zimatha kulowa mu zitsanzo kapena zitani ndi zosungunulira.
Kuonetsetsa chisindikizo choyenera: Chisindikizo chabwino chimalepheretsa kutayika kwa zitsanzo ndikusunga umphumphu.
Kuthandizira jakisoni wambiri: Septa ina idapangidwa kuti ikhale yosinthika popanda kuwonongeka.
Ndikufuna kudziwa zambiri za PTF \ / Silicone Septa, chonde onani nkhaniyi: Chilichonse chomwe muyenera kudziwa: 137 Pre-slit ptfe \ / slika slicone faps
Hplc vial septa mitundu
Zipangizo zosiyanasiyana zimakhala ndi zinthu zosiyanasiyana zomwe zimakhudza momwe amagwirira ntchito mu HPLC. Otsatirawa ndi zinthu zomwe zimagwiritsidwa ntchito kawirikawiri ku HPLC Vial Septa:
1. Ptfe (polytetrafluorothylene)
Mawonekedwe: Ptfe ali ndi kukana kwabwino kwamankhwala, ndikosavuta kwambiri ndipo imatha kupirira zikhumbo zaukali.
Kugwiritsa Ntchito: Sizoyenera ma jakisoni angapo kapena kusungitsa kwa nthawi yayitali chifukwa cholephera kuyambiranso atalipitsidwa, ndikupanga kukhala koyenera kugwiritsidwa ntchito limodzi.
Kukana kutentha Kwambiri: Kuthamangitsa kutentha mpaka 260 ° C.
Mtengo: nthawi zambiri zachuma poyerekeza ndi zinthu zina.
2. Ptfe \ / Sicone septa
Mawonekedwe: kuphatikiza kukana kwa mankhwala a PTF ndi kubwezeretsanso sisilic.
Kugwiritsa Ntchito: Zoyikidwa kuti zikhale ndi jakisoni zingapo komanso zosunga zitsanzo chifukwa cha kukonzanso kwake bwino.
Kutentha: Kuchokera -40 ° C mpaka 200 ° C.
Ochenjera: Oyenera kusintha njira.
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Mawonekedwe: Opangidwa kuti achepetse kugwirizira mukamagwiritsa ntchito singano zopingasa; amachepetsa kusintha kwa vacuum mu mbale.
Kugwiritsa Ntchito: Zoyenera kugwiritsa ntchito ntchito zomwe zimafunikira singano yoyeserera imafunikira popanda kunyalanyazidwa septum kukhulupirika.
Kutentha: Zofanana ndi PTFF \ / silicone, -40 ° C mpaka 200 ° C.
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4. .thyl mphira
Katundu: katundu wosindikiza wabwino, koma osakanikirana ndi mankhwala poyerekeza ndi PTF.
Gwiritsani ntchito: yoyenera mayankho am'madzi komanso ocheperako ochepera; itha kugwiritsidwa ntchito pofunsira ntchito zofunika kuchitika.
Kukana Kulimba Kwambiri: Zabwino pa kutentha kochepa, koma zitha kusokoneza kutentha kwambiri kapena ndi masitima ankhanza.
5. Bete woon
Katundu: Kugonjetsedwa kwambiri ndi mankhwala ankhanza ndikusunganso kuthekera kokana kubereka.
Kugwiritsa ntchito: kumaona ngati njira yomaliza chifukwa cha mtengo waukulu; yoyenera kugwiritsa ntchito komwe zinthu zina zalephera.
Kukana kutentha kwakukulu: Kugwira mtima kuwirikiza kutentha kwambiri, koma kumafunikira kusamalira mosamala chifukwa cha kulimba mtima.
Zinthu zofunika kuziganizira mukamasankha HPLC Vial SePTA
1. Kugwirizana kwa mankhwala
Chinthu choyamba kuganizira ndi kufanizira kwa ma septum zinthu zomwe mukufuna kugwiritsa ntchito. Zipangizo zosiyanasiyana zimakhala ndi zolekerera zosiyanasiyana ku mankhwala:
Ptfe (Polytetrafluorothylene): Ptyfe amadziwika chifukwa cha kukana kwake kwa mankhwala ndipo ndi yoyenera ma sol sol, kuphatikizapo mankhwala owononga. Nthawi zambiri imagwiritsidwa ntchito kuphatikiza ndi zinthu zina.
Silika: Silicone Septa amasinthasintha ndipo amatha kusinthidwa pambuyo pa singano pambuyo pa singano, ndikuwapangitsa kukhala oyenera jakisoni wobwereza. Komabe, mwina sangakhale ogwirizana ndi onse osungunulira.
Nkhunga za NYYL: Izi zikuwoneka bwino, koma zimakhala ndi mankhwala ochepetsa mankhwala poyerekeza ndi PTF.
PTFF \ / sisilicone composite: kuphatikiza zabwino zonse zonse, zomwe zimagwirizana bwino ndi kukonzanso kwa mankhwala.
2. Mtundu wosungunulira
Mtundu wa zosungunulira udzathandiziranso kusankha kwa septum:
Mankhwala am'madzi: ma sodiri am'madzi, sisilicone kapena ptfe \ / sisilicone septa amalimbikitsidwa nthawi zambiri chifukwa chogwirizana kwambiri.
Organic solts: Kwa okhazikika monga methanol kapena acetonitrile, Ptfe kapena PTFF.
Mankhwala owononga: kwa ma soportive kwambiri monga ma asidi olimba kapena zitsulo, ptfe nthawi zambiri kusankha bwino.
3. Kuchita magwiridwe antchito
Kutha kwa septum kupita kukhazikika pambuyo pa suctictuct punction ndikofunikira kuti apitirize kusakhulupirika:
Makina ocheperako vs. Zopanda Slit: Ena a Septa ali ndi ziwonetsero zothandizira kuwonongeka kwa singano, koma izi zitha kukhudza chisindikizo pakapita nthawi. Zojambula zopanda malire zimapereka chisindikizo chambiri koma lingafunike mphamvu zambiri mukalowetsa.
Kukonzanso: Onani septa yomwe ingasinthe mosamala pambuyo pa zipupule zingapo zosavomerezeka kuti zisadetsedwe ndi kutuluka.
Mukufuna kudziwa ngati mungasankhe septa mu pre-slit kapena ayi? Chonde onani nkhaniyi: Momwe mungasankhire sepga chisanachitike kapena ayi?
4. Zowonjezera ndi zopepuka
Mukamagwira ntchito ndi zitsanzo zomvera, ndizofunikira kuganizira zopereka ndi zopepuka mu zinthu za septum:
Zowonjezera zotsika: Sankhani Septa yokhala ndi otsika otsika kuti muchepetse chiopsezo cha kuipitsidwa. PTF ali ndi zowonjezera zotsika kwambiri poyerekeza ndi zinthu zochokera ku mphira.
Miyezo Yoyesa: Opanga ena amapereka deta pazowonjezera; Unikani zomwe izi zikusankha septum.
5. Kulimba kwamoto kwakukulu
Ganizirani za kutentha komwe dongosolo lanu la HPLC limagwiritsa ntchito:
Kutentha kwakukulu: Ngati ntchito yanu ikuphatikiza kutentha kwambiri, onetsetsani kuti ma septum angapirire zinthuzi popanda kuwonongeka.
Mapulogalamu a Crorgenic: Mapulogalamu a Clorgenic, sankhani zinthu zomwe zimasunga umphumphu pa kutentha kochepa.
6. Mphamvu zakuthupi
Makhalidwe a septum amathanso kukhudza magwiridwe antchito:
Kukula kwake: Thisi Septa ikhoza kupereka chisindikizo chabwinoko koma lingafunike mphamvu yambiri panthawi yolowera.
Kuumitsa: Zipangizo zofatsa zimatha kukhalanso ndi mwayi wokhalanso bwino koma zitha kugwera kuwonongeka pogwiritsa ntchito mobwerezabwereza.
Kusankha HPLC VEPTum HPTUM kumafunikira kulinganiza mosamala kugwirizana, kupirira, kutentha kwambiri kukana, komanso zosowa zapadera. Mwa kumvetsetsa katundu wa zinthu zosiyanasiyana ndi mapulogalamu awo, ofufuza amatha kusintha moyenera komanso kudalirika kwa HPLC. Kusankha koyenera sikungowongolera magwiridwe, komanso kuchepetsa zoopsa zomwe zimakhudzana ndi kuwonongeka kwa zitsanzo ndi kuipitsidwa.
Achizungu
Zauchina
