Chromatography ndi njira yofunika kwambiri mumachenjezi yomwe imagwiritsidwa ntchito kupatukana ndikuyeretsa mankhwala kuchokera osakaniza. Zina mwa njira zosiyanasiyana zomwe zilipo, Flack chromatography ndi ma promatography a chmatotography (hplc) ndi njira ziwiri zotchuka, chilichonse ndi zabwino zapadera ndi ntchito. Nkhaniyi idzayamba pansi pamayiko, zabwino, zowononga, kugwiritsa ntchito, komanso kuchita bwino kwa flambatography vs. hplc.

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1. Mfundo yogwirira ntchito

Flash chromatography ndi njira yovomerezeka yomwe imagwiritsa ntchito zosungunulira kuti adutse ndi gawo kudzera mwa mzati wodzaza ndi gawo losakhazikika, nthawi zambiri silika gel. Chitsanzo chimadzaza mzere, ndipo monga zosungunulira, zinthu zosiyanasiyana za osakaniza zimalekanitsidwa kutengera kuyanjana kwawo ndi gawo losachedwa. Njirayi imathamanga kwambiri, yopangidwa ndi mafuta omwe amayeretsedwa mphindi zochepa chabe.

Komabe, HPLC, ndi njira yovuta kwambiri yomwe imaphatikizanso kudutsa madziwo kudzera mumunsi yodzazidwa ndi gawo lakale. Komabe, HPLC imagwira ntchito pamavuto apamwamba (mpaka 4000 psi kapena kupitilira apo) ndipo nthawi zambiri amagwiritsa ntchito gawo laling'ono la tinthu tating'ono (3-5 μm). Izi zimathandiza kuti pakhale bwino kwambiri komanso kusinthasintha kuposa kufinya. Chifukwa HPLC imatha kuwongolera kutentha, kukakamizidwa, ndi mitengo yoyenda, imatha kukwaniritsa zowunikira kwambiri.

2. Zida ndi kukhazikitsa

Zipangizo zamaluso awiri zimasiyana kwambiri:

Flash chromatography imafuna kukhazikitsa kosavuta kokhala ndi mzere wofiyira ndi pampu kuti mupereke gawo la mafoni. Nthawi zambiri zimakhala zotsika mtengo komanso zosavuta kugwira ntchito kuposa dongosolo la HPLC.

Makina a HPLC ndi ovuta kwambiri, okhala ndi mapampu ang'onoatha, ofufuza (monga UV-vis), ndi makina opezeka ndi deta. Chifukwa zida za HPLC ndi njira yaulemu, ndalama zake zoyambirira nthawi zambiri zimakhala zokulirapo.

3. Kuthamanga ndi kuchita bwino

Chimodzi mwa kusiyana kwakukulu pakati pa Flash chromatography ndi kuthamanga kwawo:

Flash chromatography imatha kuyeretsa mafuta mwachangu, nthawi zambiri kumaliza kupatukana mkati mwa mphindi 30. Kuthamanga kumeneku ndikofunika makamaka kwa organic akatswiri organic omwe amafunika kusiyanitsa zinthu zina.

Pomwe HPLC ikugwira bwino ntchito mogwirizana ndi mtundu wopatukana, zimatenga nthawi yayitali kuti amalize ntchito zomwezi chifukwa cha mphamvu yake yopatukana - mphamvu iliyonse nthawi zambiri imatenga ola limodzi.

4. Kuyera ndi Kusintha

Flash chromatography ndi yothandiza kwambiri pakuyeretsa pakati, koma mwina sangapatse kuchuluka kwa kuchuluka kwa kusanthula kotsiriza. Zimatha kukhala zoyera bwino, koma mwina sizingakhale zoyenera kusazimwa zovuta momwe kuthetsa udindo ndikofunikira kwambiri.

HPLC ipambana popereka zinthu zambiri zoyera chifukwa cha kuthekera kwake kopepuka. Nthawi zambiri mulimonse momwe mungachitire ndi zosakanikirana zovuta kapena pamene kupatukana kwabwino kumafunikira.

5. Chitsanzo

Flash chromatography nthawi zambiri imapereka ndalama zokulirapo kuposa HPLC. Uwu uwu umapangitsa kukhala koyenera kuyeretsa ma consiting ambiri, komwe kumakhala kopindulitsa pa kaphatikizidwe koyambirira.

HPLC, ngakhale kuti imatha kusamalira zitsanzo zingapo, nthawi zambiri zimakhala ndi mphamvu zochepa chifukwa cha kukula kwake kwa tinthu tating'onoting'ono komanso zofunika kwambiri.

6. Malingaliro

Mtengo ndi chinthu chofunikira posankha pakati pa Flash chromatography ndi HPLC:

Flash chromatography siokwera mtengo, yonse malinga ndi zida ndi ndalama zogwirira ntchito. Ndizosavuta kukhazikitsa ndipo pamafunika kukonza pang'ono, kupangitsa kuti ikhale yokongola kwa malobotala okhala ndi ndalama zochepa.

Ngakhale HPLC ili ndi chiyero chabwino komanso chiyero, zimakhala ndi ndalama zambiri chifukwa cha zovuta za dongosololi ndi kufunika kwa zosempha zosemphana ndi mizata ndi ma sol sol.

7. Ntchito

Njira zonsezi zimagwiritsidwa ntchito mosiyanasiyana m'magawo osiyanasiyana:

Flash chromatography imagwiritsidwa ntchito kwambiri pakupanga kaphatikizidwe ka muyeso wa zinthu. Ndiotchuka kwambiri m'maphunziro omwe amaphunzitsidwa pomwe kupatukana mokakamiza zinthu kumafunikira mu kafukufuku.

HPLC imagwiritsidwa ntchito kwambiri gawo la mankhwala osokoneza bongo kuti musanthule mankhwala, kuzindikira zodetsedwa kuyezetsa kwachilengedwe, zodetsa zoyesedwa ndi chilengedwe, ndi maphunziro a biochenal pomwe pakufunika kuwunikira.

Zabwino ndi zovuta

Flash chromatography

Ubwino: Nthawi yoyeretsa mwachangu. Zida zotsika ndi ndalama zogwirira ntchito. Kukhazikika kosavuta ndi ukadaulo wocheperako wofunikira. Zitsanzo zapamwamba.

Zoyipa: Kusintha kotsika kuyerekeza ndi HPLC. Sizingakwaniritse kuchuluka kwakukulu kwa kuyesedwa kokwanira pakuwunika kotsiriza. Zovuta zochepa zomwe zosakanikirana zimafunikira kupatukana kwabwino.

Kuyenda Kwambiri Kumadzi Chromatography

Ubwino: Kuthetsa milingo yoyera ndi zoyera. Kutha kuwunika bwino zosakaniza zovuta. Kuwongolera kolondola kwa ntchito zogwirira ntchito kumathandizanso kubereka.

Zoyipa: Nthawi yocheza. Ndalama zapamwamba kwambiri komanso ndalama zogwirira ntchito. Zida zovuta zambiri zimafunikira maphunziro apadera.

Mwachidule, Flash Chromatography ndi HPLC iliyonse imakhala ndi maubwino ndi zovuta komanso zovomerezeka pamapulogalamu osiyanasiyana pamankhwala owunikira. Flash chromatography ndi yotsika mtengo komanso yotsika mtengo, ndikupanga kukhala yabwino kuti ikhale yotsuka mwachangu mu kapangidwe kake. Mosiyana ndi izi, HPLC ili ndi chivomerezi chabwino komanso chiyero, chomwe chimafunikira kugwiritsidwa ntchito mwatsatanetsatane mu mankhwala, sayansi yachilengedwe, ndi kuyezetsa kwa chakudya.

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Pomaliza, kusankha pakati pa Flash chpltography ndi zosowa zapadera, kuphatikiza kuchuluka kwa chiyero, kuphatikiza kwa bajeti, zovuta, zopinga za bajeti, ndi nthawi ya bajeti. Kumvetsetsa izi kumapangitsa ofufuza kuti asankhe ukadaulo woyenera kwambiri chifukwa cha zosowa zawo zamakhalidwe, ndikuonetsetsa zotsatira zake zabwino pakuwunika kwa chromatophphic.

M'mbuyomu:

Kodi Mungasankhe Bwanji HPLC YABWINO HPLTA ya SETTA yanu?

Ena:

Kodi HPLC imasiyana bwanji ndi GC Column?