HPLC vs Uhplc: Kusiyana kwakukulu, zabwino, komanso momwe mungasankhire ufulu wa chromomography | Ehilera
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Hplc kapena uhplc? Momwe mungasankhire ufulu wamadzimadzi wamadzi abwino

Oct. 31st, 2025
Ma tag: Hplc vs uhplc madzi a chromatography Chemical Chemistry Chromatography Air HPLC Columns Mitundu ya UHPLC kusanthula kwa labotale Kufanizira kwa chromograph Mbale za UHPLC

Kodi HPLC ili bwanji?

Makina oyendetsa ma chromatography amagwiritsa ntchito zigawo zodzaza ndi tinthu tating'onoting'ono (nthawi zambiri 3-5 μm), zimagwirira ntchito zovuta (nthawi 400-600 bar), ndipo amagwiritsidwa ntchito ndi chilengedwe. Ndizodalirika, zodalirika, komanso zogwirizana ndi zomangira zambiri zomwe zilipo.

Uhplc ndi chiyani?

Ultra-body-body fromatography imalepheretsa tinthu tating'onoting'ono (nthawi zambiri ≤2 μm) ndipo imagwira ntchito mopanikizika kwambiri (mpaka ~ 1000-100 bare kapena kupitilira). Phindu limaphatikizapo nthawi yofulumira, kuchita bwino kwambiri komanso kusinthasintha. Kwa ma labs omwe amathandizira mitundu yosiyanasiyana, kusanthula kapena kufuna kuchuluka kwake, UHPLL ndi njira yolimba.
Kusiyanitsa kwakukulu pakati pa HPLC ndi UHPLL

Palamu

Hplc

Uhplc

Kukula kwa tinthu

3-5 μm

≤ 2 μm

Kukakamizidwa

Mpaka ~ 400-600 bar

Mpaka ~ 1000-1500 bar

Kuthamanga & kutulutsa

Wasaizi

M'mwamba

Kuvomeleza

Wofanana

Onjezera

Ndalama zolipirira

Chepetsa

Okwezeka



Nthawi yogwiritsa ntchito HPLC

HPLC ndi yabwino pamene ma sampuli anu atrix ndi ofunikira, zomwe zimafuna kuti zibwereke ndizosavuta, zopinga za bajeti zilipo, ndipo mumalinganiza njira zotsimikizika, zokhazikika. Ngati ntchito yanu imagwirira ntchito yaying'ono molekyu, QA \ / mabotolo ophatikizika kapena ma protocols okhazikitsidwa bwino, HPLC imakhalabe ndi chisankho cholimba.


Mukamagwiritsa ntchito UHPLC

Ngati mungagwire zitsanzo zovuta (E.g. Komabe, dziwani kuti uhplc imafuna misiri yolimba kwambiri (voliyumu yotsika kwambiri, pampu yolimba kwambiri, yoyesedwa yolimba kwambiri, yovuta kwambiri) ndi kupezeka koyambirira koyambirira.


Zovuta

Kuyenda kuchokera ku HPLC ku Uhplc kumathandizanso kusankha zochita. Kusankha mbale zapamwamba kwambiri, autosampr syringes, zosefera, ndi zigawo zinthu zina kwambiri mu UHPLC. Mwachitsanzo, labu pogwiritsa ntchito Uhplc iyenera kuganizira zamitengo yotsika-ahplction


Bwanji kusankha chromatography's chromatography

Ku Zhejiiang Airlogloglogy Co., Ltd., Timathandizira makope ambiri a HPLC ndi UHPLC ndi akatswiri athu, zopangidwa. Kupereka kwathu kumaphatikizapo mbale zotsimikizika, septa yolumikizidwa, zosefera zoyera ndi oem \ / odm solution. Pogwiritsa ntchito aijaren zomata, ma labs amagwiritsa ntchito zodalirika, kuchepetsedwa kuipitsidwa kwina komanso kubereka kwabwino.


Onse a HPLC ndi UHPLL ali ndi malo awo mu labotato wamakono. HPLC imangokhala ndi mtengo wogwira ntchito molimbika, pomwe uhplc imathandizira kuthamanga ndi kusintha kwa zinthu zambiri zofunika kwambiri. Chinsinsi chake ndikugwirizanitsa chida chanu, chogwirira ntchito, chomata, komanso bajeti pazolinga zanu.



Mafunso Omwe Amafunsidwa Kwambiri (FAQ)

Kodi Uhplc nthawi zonse ndi bwino kuposa HPLC?

UHPLC imapereka chiwonetsero chambiri komanso kusanthula mwachangu, koma zimabwera ndi mtengo wokulirapo komanso zofunikira kwambiri - kotero "zabwinoko" zimatengera pulogalamu yanu inayake.


Kodi ndingagwiritse ntchito mizati yomweyo ya HPLC ndi UHPLC?

Wamba ayi. Mitundu ya UHPLC imakonzedwa kuti ikhale yovuta kwambiri komanso tinthu tating'onoting'ono, komanso kuchuluka kwa HPLC sikungachite bwino kapena mosamala mu UHPLC.


Ndi zinthu ziti zomwe ndingakweze ndi kukweza Uhplc?

Yambirani pa zotsika kwambiri-adsorption otsika, zosefera za ultra-kuyeretsa ndi makina ochepa otsika, kuti mukhale ndi mawonekedwe a nsonga ndi kubereka ku Uhplc.

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