HPLC AMENCT CRUSTELD - potsogolera

Chidule cha Zitsanzo

Kudzipereka kosangalatsa kumaphatikizapo ntchito zonse musanayambe jakisoni. Kupatula zolemera, kusungunuka, komanso kuchepetsedwa, kumatanthauzanso njira zotsatirazi:

• Kufinya
• Kuthamangitsa
• DIrivatation (Pre-Crivatation)
• Madzimadzi a chromatography (otsika kwambiri a chromatography)

Kudzichitira zodzikonza kumatha kuchitidwa nokha pamanja kapena ovomerezeka. Kodi mumazolowera kusamalira makasitomala?

Kuleka

Zitsanzo zina sizingakhale zabaliridwa mwachindunji. Derivatation amafunikira kupanga mankhwala popanda uV kapena flurescence wowoneka bwino ndi maluso a UV kapena Fluorescence.

• Amasintha chidwi
• Imathandizira kulekanitsa (kusintha kwabwino)

Nkhani zomwe zingatheke zimaphatikizapo kuwonongeka kwa zitsanzo, kuipitsidwa, zochita zosakwanira, kapena zinthu zingapo, zomwe zimakhudza kulondola kapena kufotokozera zolakwa.

Zosasinthika

Zitsanzo za LC ziyenera kukhala yunifolomu ndipo tinthu tosamasulidwa; tinthu tating'onoting'ono titha kuwononga jakisoni kapena kutseka mzati. Njira zimaphatikizapo:

• Gwedezani zitsanzo zisanayambe jakisoni ndikuyang'ana tinthu tating'onoting'ono, Turbidity, kapena emulsions
• Ngati tinthu tating'onoting'ono tiwonedwe, osefa zitsanzo pogwiritsa ntchito nembanemba yomwe imasunga ≥0.15μm tinthu

Nkhani zomwe zingatheke: Kuyipitsitsa kwa zitsanzo, ma adsorption amachepetsa chinthu chophatikizira, ndikusinthasintha kwa Evatery Kuyambitsa Zolakwika.

Njira Zowonjezera

Kuchulukitsa Kusakanikirana Kusakaniza Kuchokera ku Co-ma sodi kapena kuchotsa zinthu zomwe zimasokoneza zinthu (mwachitsanzo, mapuloteni). Ordic solt iyenera kukhala yotsika kwambiri, yosasunthika, yotsika kwambiri m'malo mwazinthu, ndikusungunuka bwino.

Ma solformanti wamba: Diethyl ether, ethyl acetate, dochloromethane, chloroform, benzene, kapena kusakhazikika.

Pambuyo pochotsera, zitsanzo zimatha kulowetsedwa mwachindunji kapena kukhazikika powonjezera kusanthula komanso kukhudzidwa.

Madzi osungunuka amadzi

1. Acidic contracles ndi mchere: kutulutsa zodetsa ndi zosungunulira, kusintha kwa acidic, ndiye kutulutsa kapena kubaya jekeseni; kapena wowuma pansi pa N₂ ndikusungunuka mu zosungunulira yoyenera musanayambe.
2. Zigawo zoyambira ndi mchere: Kutulutsa zonyansa ndi zosungunulira, kusintha kukhala koyambira, kenako kutulutsa kapena kubaya; kapena wowuma pansi pa N₂ ndikusungunuka mu zosungunulira yoyenera musanayambe.
3. Zophatikizidwa ndale: kuchotsa zonyansa ndi zosungunulira zachilengedwe, ndiye pendani mwachindunji pobwezeretsa chromatography.

Mafuta osungunuka mafuta

Tingafinye ndi zosungunulira kapena zamkati mwachindunji; kapena wowuma pansi pa N₂ ndikusungunuka mu zosungunulira yoyenera musanayambe.

Zodetsa nkhawa

Magwero osokoneza bongo akuwunika:

Kukonzekera kwanzeru

• Konzani yankho la mkati: Gwiritsani ntchito batani limodzi muyezo ndi zitsanzo kuti muchepetse zolakwika
• Zinthu ziyenera kukhala za chromatography-giredi
• Sungani mayankho oyenera m'mabotolo a bulauni pamtunda wotsika
• Kwa mavitamini, gwiritsani ntchito ma volluoltric flatssic kapena kuteteza ku kuwala

Mavuto Ofala Pazosangalatsa ndi Zothetsera