HPLC (ma promatography) ndi GC (ma chromatography) onsewa ndi njira zamphamvu zowunikira zogwiritsidwa ntchito kupatula, kuzindikira, komanso kuchuluka kwa zitsanzo zosiyanasiyana. Komabe, zimasiyana kwambiri poyerekeza ndi ntchito, zida, ndi ntchito. Nkhaniyi ikufotokoza mosiyanasiyana pakati pa mzati wa HPLC ndi GC, kuyang'ana kapangidwe kake, magwiridwe antchito, komanso oyenera mitundu yosiyanasiyana ya kusanthula.

Kapangidwe kanu

HPLC Columns

Ming'alu ya HPLC imakhala yofupikira komanso yopambana kuposa zilonda za GC. Amakhala mpaka 30 cm kutalika ndikukhala ndi mainchesi amkati kuyambira 2.1 mm mpaka 8 mm. Kuyika mkati mwa zipolopolo za HPLC kumakhala ndi tinthu tating'onoting'ono (nthawi zambiri zochepera 5 mic) yomwe imapereka malo akuluakulu oti mugwirizane ndi zitsanzo za zitsanzo. Kulongedza katundu wa mizamu iyi kumawalola kuti azisiyidwa moyenerera mitundu yosiyanitsa ndi mankhwala awo.

Mawonekedwe Aakulu:

Kutalika: Mpaka 30 cm

Diameter: Nthawi zambiri pakati pa 2.1 mm ndi 8 mm

Zipangizo za kunyamula: tinthu tating'onoting'ono (mwachitsanzo, silika) ndi zinthu zosiyanasiyana zapamwamba zoyenera kuzipaka zosiyanasiyana (E.g. Yobwezeretsedwa, gawo labwino).

Ng'ombe za GC

Columns GC, mosiyana, ndi zazitali komanso zazing'ono, nthawi zambiri mpaka kutalika kwa 100 m mmambo kuchokera pa 0.1 mm mpaka 1 mm. Amatha kugawidwa m'magulu awiri: omwe ali ndi miyala yamiyala ndi zipilala. Nkhono zodzaza ndi gawo lokhazikika kapena madzi okutidwa ndi chithandizo cholimba, pomwe ma capillary Courns ali ndi kanema woonda wa gawo la station lomwe lili pakhoma lamkati.

Mawonekedwe Aakulu:

Kutalika: mpaka 100 m

Diameter: Nthawi zambiri pakati pa 0,1 mm ndi 1 mm

Mitundu: Columns yolumikizidwa (gawo lolimba kapena lamadzimadzi) ndi mipiringidzo ya capillary (yotseguka tulo).

Gawo lam'manja

Kuyenda Kwambiri Kumadzi Chromatography

Ku HPLC, gawo lam'manja nthawi zambiri limakhala ndi madzi osungunuka kapena osakaniza polar kapena polar solant. Mapiritsi wamba amaphatikizapo madzi, methanol, acetonitrile, ndi zodula zosiyanasiyana. Kusankha kwa gawo lam'manja ndikofunikira chifukwa kumakhudza kulumikizana pakati pa kafukufukuyu komanso gawo lokhazikika mkati mwa mzati.

Gasi chromatography

GC imagwiritsa ntchito gawo la mafoni, lomwe limakonda kwambiri mpweya ngati helium kapena nayitrogeni. Chitsanzo chikuyenera kukhala chosasinthasintha kuti chitheke pomwe chimayambika pamunsi. Chofunikira ichi chimatanthawuza kuti GC ili yoyenera kusanthula zinthu zosasunthika, pomwe HPLC imatha kuthana ndi zinthu zingapo, kuphatikizapo mankhwala osagwirizana.

Kupatukana

Kuyenda Kwambiri Kumadzi Chromatography

HPLC imalekanitsa mankhwala kutengera kucheza kwawo kwa gawo logwirizana ndi gawo la mafoni. Mitundu yosiyanasiyana ya chromakography ikhoza kugwira ntchito:

Kusintha gawo la chromatography: ntpolalary station gawo lokhala ndi gawo la polar.

Gawo labwinobwino la chromatography: gawo la polar station yokhala ndi gawo la mafoni.

Zikani Kusinthanitsa kwa Chromatography: Kulekanitsa mitundu yolipiridwa mogwirizana ndi kuyanjana kwawo ndi gawo lokhazikika.

Kukula kwa cromatography: kulekanitsa mamolekyulu okhazikika pamlingo.

Gasi chromatography

M'magesi chromatography, kupatukana kumachitika makamaka chifukwa cha kusiyana kwa malo osungirako komanso owotcha. Mafuta omwe amatuluka mosavuta kuchokera pachilumbu choyamba, pomwe ochepa kwambiri amasasunthika adzatenga nthawi yayitali. Kuyanjana pakati pa kuwunikira komanso gawo lakumanja lingakhudzenso nthawi yosungira.

Chidwi ndi kusinthana

HPLC Kukhuta

HPLC nthawi zambiri imakhala ndi chidwi kwambiri kwa mankhwala osasunthika chifukwa imatha kusanthula zitsanzo zochepa popanda zosintha. Pogwiritsa ntchito zingwe zazing'ono zazing'ono mu HPLC Columns imapereka malo okulirapo kuti azithana.

Gc kumva bwino

Popeza ma chromatotography amatha kuwunika mwadzidzidzi kudzera mu EXPAPATION, imatha kukwaniritsa chidwi chachikulu cha osasunthika. Ming'alu ya capillary nthawi zambiri imakhala ndi njira yabwinoko kuposa kuchuluka kwa mizere yodzaza chifukwa cha kutalika kwatali komanso mainchesi.

Ntchito za HPLC ndi GC

Mapulogalamu a HPLC

HPLC imagwiritsidwa ntchito m'minda yambiri chifukwa cha kusiyanasiyana kwake:

Kusanthula kwa mankhwala: Kugwiritsidwa ntchito poyesa mankhwala osokoneza bongo komanso kuwongolera kwapadera.

Kuyesa kwachilengedwe: Unikani zodetsa m'madzi ndi dothi.

Kuyesa kwa Chitetezo cha Chakudya: Kuzindikira zodetsa ndi kutsimikizira za chakudya.

Biotechnology: Yesani mapuloteni ndi ma acid acid.

Mapulogalamu a Chromatography

GC imagwiritsidwa ntchito kusanthula zokongoletsa zachilengedwe:

Kusanthula kwa chilengedwe: Kuyeza mankhwala osokoneza bongo ophatikizika m'madzi ndi madzi.

Sayansi: Kusanthula zida pa milandu yaupandu.

Makampani ogulitsa a Perrochemical: Tanthauzo la hydrocarbons mu mafuta.

Kusanthula ndi kusanthula kwa fungo: Kuzindikira zinthu zomwe zimayambitsa mavuto.

Mwachidule, HPLC ndi GC ndi njira zosiyana za cromiotographic zomwe ndizoyenera kusanthula mitundu yosiyanasiyana malinga ndi kapangidwe kake kameneka, kagwiridwe ka foni, kudzipatula, kugwiritsa ntchito, komanso luso losinthana. HPLC ndi yoyenera pazinthu zosasunthika kapena mwamphamvu zomwe zimafunikira gawo lamadzi lamadzimalo, pomwe GC imayenda bwino posanthula zinthu zosasunthika pogwiritsa ntchito gawo la mafoni. Kumvetsetsa izi kumapangitsa ofufuza kuti asankhe njira yoyenera kuti akwaniritse zosowa zawo zapadera, ndikuwonetsetsa zolondola pamagawo osiyanasiyana asayansi.

Kodi mukudziwa kusiyana pakati pa VPLC Vials ndi Mbale za GC? Onani nkhaniyi: Kodi pali kusiyana kotani pakati pa mbale ya HPLC ndi Mbale za GC?

M'mbuyomu:

Flash Chrometography vs. hplc: kufananizidwa mwatsatanetsatane

Ena:

Kodi kufooka kumafanana bwanji ndi njira zina zopendekera pansi