Pamankhwala omveka bwino, ma chromatography (GC) njira wamba. Ikopecheza, kusiyanitsa, ndi njira zovuta zophatikizira zosakhazikika komanso zosasunthika. Pamtima pa chimango chilichonse cha GC chimagona cholumikizira. Ndi ngwazi yosagwirizana. Imakhala gawo lofunikira potsimikizira chiweruziro ndi mtundu wosasunthika womwe mumabwera.

Mbale za GC ndi zotengera zazing'ono. Amapanga zitsanzo zothetsera. Amapangidwanso kuti ayambitse mayankho mu magemu a chromatograph. Zosavuta izi ndi kiyi. Amasunga mtundu wanu wa GC ndi kusasinthika. Amasintha zitsanzo zomwe zimachitika, zimakopa katswiri wosankhidwa, komanso amakhudza dongosolo.

Kusankha kwa GC Vial kumatha kukhudza kwambiri pabwino kwa chromatographic. Cholinga chilichonse cha kapangidwe kake chingakhudze kusanthula kwanu. Izi zimaphatikizapo kapangidwe kazinthu komanso zoyipa. Izi ndi zomwe zimapangitsa kuchira. Amakhudzanso kulondola komanso kubwereza zotsatira zanu.

Chofunika Kwambiri Kufunika Kwa Kufunika kwa Mbale Zapamwamba Mu Clobotographics? Onani nkhani yathu yokwanira:Chifukwa chiyani mbale zamtchire ndizogwiritsidwa ntchito mu chromatography? 12

Mitundu ya GC Vial

Mbale za GC zimabwera m'mitundu ingapo. Aliyense ali ndi mikhalidwe yapadera ndikugwiritsa ntchito.

1. Mbale zapamwamba kwambiri:

Awa ndi mtundu wotchuka wa GCMbale zapamwambaBwerani m'mafuko ndi zida zambiri, monga galasi, polypropylene. Amakhalanso ndi zosankha zambiri zotsekera. Izi zimawapangitsa kusankha bwino kwa ntchito zambiri za GC. Chipewa chili ndi ulusi. Zimapanga chisindikizo. Izi zimasiya kuzimiririka komanso kuipitsidwa. Zimathandiziranso kusamalira kosavuta ndi zoyendera. Mbale zapamwamba zapamwamba ndioyenera kwa buku lonse komanso lokhalo la gc.

2. Mbale zapamwamba kwambiri:

Mbale zapamwamba kwambirigwiritsani ntchito aluminium kapenaBurble Extabper. Kuti mupange chisindikizo chotetezeka, chithokomi chomwe chimayimitsidwa m'khosi la vial. Kapangidwe kameneka kamakonda kusanthula. Ayenera kukhala ndi zisindikizo zopanda ungwiro chifukwa cha zosinthika. Chidindo choponderako ndi chokhacho ndipo chimawonetsa kusokoneza. Izi zimapangitsa kuti zigawenga zisankhe bwino kwambiri pazomwe zimatulutsa kapena zopangira gc zokha. Izi ndi zomangira pomwe zitsanzo kukhulupirika ndizofunikira kwambiri.

3. Mbale. Mbale:

Galasi limapangitsa mbale izi. Zili ndi kusanthula kwa mutu wa GC. Ali ndi voliyumu yayikulu. Izi zimathandiza malo osungira zitsanzo zam'mutu woposa madzi kapena zitsanzo zolimba. Kusanthula kosasunthika kumayambira pang'onopang'ono kudzera pa mipata yam'mutu. Amalumikizana ndi ma genetic angwiro. Ma vayorizi, kenako amalowetsedwa mu GC. Mtundu wamtunduwu ndi wofunikira pakusanthula mankhwala osasunthika. Izi zitha kukhala zovuta kuyambitsa pogwiritsa ntchito njira zachikhalidwe zamadzimadzi.

4. Mbale ya Micro:

Mimba ya Micro imadziwikanso ngati maiwo kapena olowera. Awa ndi ovala owuma pang'ono omwe amalowa mkati mwa GC yayikulu. Pamene kuchuluka kwa zitsanzo ndizochepa, anthu amagwiritsa ntchito. Izi ndi zoona kwa owunikira omwe amafunikira kuyendetsa bwino. Mwachitsanzo, kuchepetsedwa kapena kusamala. Mitengo ya micro imathandizira kuchepetsa kutayika kwa zitsanzo. Amawonetsetsa kuti kusamutsa zitsanzo zazing'ono zazing'ono kulowa mu GC. Izi zimawapangitsa kukhala amtengo wapatali. Amasunga zitsanzo zamtengo wapatali ndikugwira ntchito ndi oyang'anira.

Mukamatola GC Vial kuti mugwiritse ntchito, muyenera kuganizira zinthu zingapo. Izi zikuphatikiza mawonekedwe a zitsanzo za zitsanzo. Komanso, kugwirizana ndi dongosolo lanu la GC ndi zofunikira zilizonse. Kusankha vial yoyenera kumatsimikizira kulondola ndi kusinthasintha kwa zotsatira za GC. Zosasintha pazosowa zanu ndikusankha mtundu wabwino wa vial.

Kuonetsetsa chisindikizo choyenera ndikamagwiritsa ntchito Mbale. Werengani chitsogozo chathu posankha chipewa choyenera kuti muchepetse kukhulupirika ndikuletsa kutaya kapena kuipitsidwa panthawi ya chromatographic: Kodi mukusankha chipewa choyenera cha Value?

Mitundu yodziwika ya ma chromatography

Ma chromatography ndi njira yowunikira yosinthika. Itha kuthana ndi mitundu yambiri yachitsanzo komanso mitundu ya anthu ofufuza. Musankha dongosolo la GC kutengera zitsanzo zanu ndi kusanthula. Ganizirani zovuta zawo, ndipo ndi kudzipatula kwanu.

1. Ma chromatography.

Ichi ndiye mtundu wofala kwambiri wamagesi. Ofufuzawo amagwiritsa ntchito kuti apeze mbali zina za osakaniza. Magulu a GC nthawi zambiri amakhala ndi mitundu ingapo yazowunikira. Izi zikuphatikiza zowunikira zojambula zamagetsi (fid). Amaphatikizanso mafuta ojambula (TCD), ndi mawonekedwe akulu (MS). Chisankho chimatengera owunikira ndi zosowa zapadera. Kupenda GC kumapereka zolekanitsidwa bwino komanso kupezeka kwanthawi. Ndi ntchito yantchito zambiri. Izi zimaphatikizapo kusanthula chilengedwe. Amaphatikizanso kuyesa zakudya ndi zakumwa. Amawongolera mtundu wa mankhwala, ndikuwunika njira zamankhwala.

2. GC yolapa:

Asayansi akukonzekera Phumushoni GC kuti adzipatule ndikuyeretsa mankhwala. Amagwiritsa ntchito zida zazikulu ndi mavidiyo kuposa kuwunika GC. Anthu nthawi zambiri amagwiritsa ntchito njirazi kupanga mfundo. Amazigwiritsanso ntchito kuti alekanitse ma isomers kapena kuyeretsa mankhwala osanthula kapena kaphatikizidwe. GC yolembedwa imalola kutolera tizigawo ting'onoting'ono. Mutha kuzigwiritsa ntchito pazantchito zambiri pambuyo pake. Mwachitsanzo, kupeza kapangidwe kawiri kapena mafotokozedwe ake.

3. Mutu wa GC:

Njira yapadera iyi ya GC. Ofufuzawo amagwiritsa ntchito posankha mankhwala osokoneza bongo komanso osasunthika. Imagwira ntchito pa zitsanzo zolimba kapena zamadzimadzi. Mutu wa GC GC umasanthula gawo la gasa (mutu) pamwamba pa chitsanzo. Maukadaulo amalekanitsa mpweya uwu ndikuyika mu GC System. Izi zimapangitsa kuti mupeze mankhwala. Amatha kukhala ovuta kusanthula kugwiritsa ntchito bongo kapena jekeseni wolimba. Pamutu Gc ndi yabwino kusanthula zosintha zachilengedwe (vocs) ndi zotsalira. Ndizabwinonso kwa osankhidwa ena osagwirizana. Zimagwira bwino ntchito zosakaniza zovuta. Zosakaniza izi zimaphatikizapo za mankhwala opangira mankhwala, chakudya, ndi chilengedwe.

4. GLIDIMIMAST GC (GC × GC):

Mu kukhazikitsa uku, zitsanzo zomwe zimaperekedwa polekanitsidwa kwa 2D. Izi zimathandiza kuti zitheke bwino komanso chizindikiritso chosiyanasiyana. Makina a GCXGC amagwiritsa ntchito zigawo ziwiri ndi magawo osiyanasiyana. Amawalumikizani motsatizana kuti alengeni mphamvu ndi kusanza. GCXGC imatha kukwaniritsa mphamvu zambiri. Zimachita izi pophatikiza luso la mizere iwiriyo. Imathanso kuthetsa chidwi lomwe limatha kugwirizana mu dongosolo la GC imodzi. Njirayi ndi yabwino kupenda zitsanzo zovuta. Izi zimaphatikizapo zinthu zopangira mafuta, mafuta ofunikira, komanso zopanga zachilengedwe. Amafunikira kuchuluka kwapamwamba.

5. Gasi chromatography-misa spectrometry (GC-MS)

Mafuta a chromatography ndi misa misa ndi chida champhamvu. Amatha kuzindikira ndikuyeza mankhwala osadziwika. Makina a GC-MS amasangalala ndi maluso a GC. Amapezanso zolembedwa ndi zotsimikizika zochokera ku misa. Makina amapeza ntchito mu chilengedwe, zokopa, ndi minda yama metabolomics. Gc amalekanitsa zitsanzo m'magawo ake. Wotchinga wa MS amazindikira chilichonse. Zimawalimbikitsa kutengera unyinji wawo wapadera. Izi zimathandiza kuti kusanthula kokwanira kwa zosakaniza zovuta.

Mukuyang'ana kuwunika kwathunthu kwa mbale za mutu? Buku lathu lakuya limaphimba chilichonse chomwe muyenera kudziwa - kuchokera pamagetsi ndi njira zosankhira kwa mitengo yamagalimoto yofanana ndi GC ndi HPLC: Maupangiri Okwanira a Mbale: Zosankha, kusankha, mtengo, ndi kugwiritsa ntchito

Mosasamala kanthu za njira ya GC yomwe imagwiritsidwa ntchito. Kusankha kwa GC Vial ndikofunikira. Zimatsimikizira kupambana ndi kudalirika kwa mapangidwe anu. Sankhani vial yomwe ikufanana ndi mtundu wanu, zakuthupi, ndi zofunikira. Izi zitha kupititsa patsogolo zitsanzo zoyambitsa komanso kuchira. Ikuwonjezeranso mtundu ndi kubereka kwa deta yanu ya GC.

M'mbuyomu:

Mitengo ya HPLC ndi mtengo wa Caps: Kuwongolera kwakuya

Ena:

20ml riverses kugwiritsa ntchito