Njira zodziwika bwino za GC-ms

Mafuta a chromatography-misa spectring (GC-MS) ndi njira yamphamvu yowunikira yomwe imagwiritsidwa ntchito posankha zosinthika ndi semivolatile. Kutengera mtundu wa zitsanzo ndi zomwe akufuna, njira zosiyanasiyana zomwe zingagwiritsidwe ntchito bwino kukonzekera zitsanzo. Otsatirawa ndi njira zodziwika bwino zomwe zimagwiritsidwa ntchito kukonza zitsanzo zaKusanthula kwa GC-MS:

Mukufuna kudziwa zambiri za kusiyana pakati pa LC-MS ndi GC-MS, chonde onani nkhaniyi:Kodi pali kusiyana kotani pakati pa LC-MS ndi GC-MS?

1. Kukonzekera kwamadzimadzi

Mankhwala: Zitsanzo zamadzimadzi zimangosungunuka mu zosungunulira pang'ono monga methanol, acetone, kapena dichloromethane kuti akwaniritse kuchuluka kwa 0.1 mpaka 1 mg \ / ml. Izi zikuwonetsetsa kuti sampuli ikugwirizana ndi GC System ndipo imachepetsa chiopsezo chotchera.

Kusanthula: Asanasanthule, sampleyo iyenera kutsukidwa kuti ichotse tinthu tating'ono tomwe tingasokoneze kusanthula. A0,22 μmnthawi zambiri amagwiritsidwa ntchito.

Mwachitsanzo: Pazitsanzo zomwe zingakhale ndi zinthu, centrifuagation zitha kuthandiza kulekanitsa madzi ndi zinthu zilizonse zosafunikira musanasanduke ndi vial.

2. Kukonzekera Molimba

Kusintha: Zitsanzo zolimba ziyenera kusungunuka mu zosungunulira zokwanira. Onjezani ndalama zochepa (mbewu zochepa) zolimba mpaka mu valvent yosungunulira ndikusintha kangapo kuti zitsimikizike zowonongeka.

Derivatalization: Pa mankhwala osasunthika kapena polar, kupulumuka kungakhale kofunikira kupititsa patsogolo kusamvana. Izi zimaphatikizapo kusintha kwa makina kuti mufufuze kuti zitheke kuwunika kwa GC.

3. Kusanthula Mutu Wam'mutu

Mphepo yambiri: Munjira imeneyi, chosindikizidwa chosindikizidwa chomwe chili ndi kutentha nthawi zonse kuti chichepetse zinthu zomwe sizikusintha. Nthawi yomweyo kufanana kufikiridwa, nsonga iyi imatha kusanthula pogwiritsa ntchito syringe yolimba.

Mphamvu yamphamvu (poyeretsa ndi msampha): Njira iyi imaphatikizapo kupatsira mpweya wosakhazikika kudzera pa zitsanzo kuti zithandizirenso kuchotsa. Njirayi imachulukitsa kwambiri potengera osanthula asanasanthule.

Mukufuna kudziwa zambiri chifukwa chifukwa chiyani pali mbale zamatumbo omwe amagwiritsidwa ntchito mu chromatography? Chifukwa chiyani mbale zamtchire ndizogwiritsidwa ntchito mu chromatography? 12

4. Njira Zapachikulu

Gawo lolimba microxaxtrated (Spme): Spme imagwiritsa ntchito chiberekero chojambulidwa ndi gawo la m'zigawo zotengera kusanthula kwa madzi kapena gasi. Njirayi imalola kuti kusanthula mwachindunji popanda kufunikira kwa ma sol sol ndipo ndikofunikira makamaka kwa osasunthika.

Madzimadzi amadzimadzi (le) komanso gawo lolimba.

5. Malangizo oyang'ana

Kuyeretsa kwa nayitrogeni kumagwiritsidwa ntchito kuwunikira zitsanzo za kusintha kwa nayitrogeni pansi pa nayitrogeni, zomwe zimathandizira kuchepetsa kuchuluka kwa chiwonetsero.

Kukonzekera Kukonzekera

Onetsetsani kuti mafano ogwiritsa ntchito ndi osinthika komanso oyenera a GC-MS; Madzi ndi osasunthika osungunuka ayenera kupewa.

Zitsanzo siziyenera kukhala ndi ma acid aliwonse amphamvu, misasa, mchere, kapena zodetsa zina zomwe zitha kuwononga chipilala cha GC kapena kusokoneza kusanthula.

Zitsanzo zomaliza ziyenera kukhala zopanda ma 5 ndipo zimakonzedwa muMbale zagalasi kuletsa zotupa za pulasitiki.

Kuti mumve zambiri pa Autosambir Mbale za Chromatography, fotokozerani nkhaniyi: 2 ml Autosambir Mbale za Chrometography

Mapeto

Njira zotsatsira bwino ntchito ndizofunikira pakuwunika kwa GC-MS. Njira iliyonse ili ndi maubwino ake ndipo imagwiritsa ntchito njira zina kutengera mtundu wa zitsanzo ndi zandamale. Mwa kugwiritsa ntchito njirazi, kusanthula kumatha kukonza kulondola, kuzindikira, ndi kubereka kwa zotsatira zawo, pamapeto pake kupeza deta yodalirika monga kuwunikira zachilengedwe, chitetezo cha chakudya, ndi mankhwala osokoneza bongo, ndi mankhwala osokoneza bongo.