Momwe mungasankhire pakati pa HPLC ndi GC Mbale? Kuyerekeza kwazinthu zakuya ndi zogwirizana

Chiyambi

Mu kusanthula kwa cromatographic, kusankha makina oyenera kumapangitsa kulondola kwa zolondola zanu. Nkhaniyi ikufotokoza mokwanira za kusiyana pakati pa HPLC ndi GC Models, zamakhalidwe ogwiritsira ntchito, zinthu, ndondomeko zopindika, komanso kulumikizana - Kukuthandizani kuti musinthe pasayansi.

I. Kusiyana kwakukulu pakati pa HPLC ndi GC Mbale

1. Ma prenarios

Mbale za HPLC: Makamaka kugwiritsidwa ntchito kwambiri kwa ma chpomatography (HPLC) ndi LC-MS, oyenera osasunthika, osakhazikika, kapena zitsanzo zopepuka.

Mbale za GC: Kugwiritsidwa ntchito mu chromatography (GC) ndi GC-MS, Kufunikira Kulimbana Kwambiri (Nthawi zambiri> 300 ° C) ndi Kugwirizana ndi Volatile Malekezero.

2. Zinthu zakuthupi ndi kapangidwe

Mitundu yam'manja:

HPLC:Nthawi zambiri gwiritsani ntchito zotchinga pamwamba kapena nsonga zowoneka bwino, zabwino zopezeka mobwerezabwereza komanso kusangalatsa.

GC:Nthawi zambiri miyala yapamwamba kwambiri yokhala ndi ma aluminium caps ndi ptfe \ / slika pepta ya mapiri opindika kwambiri.

Mukufuna kuphunzira za njira zisanu zoyeretsa ma chromatography? Dinani apa kuti muwongolere

Kusankha Zinthu:

HPLC:Nthawi zambiri zopangidwa ndi galasi lalitali-loyera, ndi mitundu ya Amber kuti azolowere.
GC:Imafunagalasi othamanga kwambiri-borosiltil-borosiltil ofala, ndi pulasitiki ya PFA yomwe imagwiritsidwa ntchito pamapulogalamu apadera.

Ii. Maganizo a Chromatography Vial

1. Zida zagalasi

Magalasi a Borosil: Kutentha kwambiri ndi kutentha kosagwirizana; Oyenera kuwunika kwa HPLC ndi GC.
Galasi la amber: Amapereka chitetezo chopepuka cha zitsanzo zowoneka bwino.

2. Zida za pulasitiki

Polypropylene (pp): Mtengo wogwira ntchito komanso woyenera kugwiritsa ntchito kutentha kwambiri; zosungunulira zochepa komanso kukana kutentha.
Offersocallocy (PFA): Kukana kwa mafuta ochulukirapo komanso kusamvana kwamankhwala; Zabwino kwambiri kuti tisanthule ndi kusanthula kwamphamvu kwambiri kwa gc.

Ndikufuna kufufuzaKodi GC mutu ndi chiyani? Dinani apa kuti muyang'ane

3. Cap ndi Septa

PTFF \ / Silicone septa: Pewani kulowerera ku HPLC; Sungani mawonekedwe pansi pa kutentha mu GC.
Njira Zosindikizira: Zikopa za GC CRIP zimafunikira zida zapadera, pomwe HPLC Scress Capss ndizosavuta kugwira.

Iii. Nkhani zogwirizana komanso momwe angazipewe

1. Makina osinthira

Autosambler Kugwirizana: Onetsetsani kuti kutalika kwa Vial ndi diameter kumayenderana ndi ma tray (E.g., 2 ml moyenera Mbale).
Septa punction kukana: Kwa jakisoni pafupipafupi ku HPLC, sankhani Septa yokhala ndi kutalika kwakukulu (E.g., PTF.

Kukumana ndi zipsera za vial ndi Septa, muyenera kudziwa Cleak apa

2. Kuwongolera chiopsezo

Kupewa Zowonjezera: Pakutentha kwambiri kwa gc, sankhani bala kapena PLA Mbale.
Kuletsa kuipitsidwa: Gwiritsani ntchito mtengo wotsimikizika wakale (E.g., USP Kalasi ya A).

Iv. Chitsogozo Chogula: Kusankha vial yoyenera kwa HPLC ndi GC

Tanthauzirani Zosowa Zoyesera: Ganizirani zamtundu wamadzi, chimbudzi chambiri \ / chidwi-chidwi, ndi jekele skisqueker.
Fananitsani zida: Onetsetsani kuti mtundu woyenera wa khosi, voliyumu, ndi autosambir.

Komabe osatsimikizarial vial vs vs snap vial vs scress kapu vial, momwe mungasankhire? Ma lungamoni mfundo pano

Onaninso miyezo yamakampani: Gwiritsani ntchito Mbale ndi USP kapena ISO chitsimikizo cha kusasinthika.
Kuwunika kwa Ndalama: Kuti muyese pafupipafupi, sankhani Mbale zagalasi Yotsika; Pofuna kusanthula, kusankha zida zanzeru.

V. Faq - Mafunso Odziwika

Q: Kodi Mizere ya HPLC ikhoza kugwiritsidwa ntchito popenda kwa GC?
A: Ngati opangidwa ndi galasi ndi oyendetsa kutentha (mwachitsanzo, mpaka 400 ° C), atha kugwiritsidwa ntchito kwakanthawi. Komabe, kugwiritsa ntchito nthawi yayitali pansi pa kutentha kwambiri sikulimbikitsidwa.

Q: Kodi mungapewe bwanji kuipitsidwa?
Yankho: Sankhani zotsimikizika zotsukidwa kapena kuyeretsa ma ultratoraphically pogwiritsa ntchito ma solatot.

Q: Kodi Mbale Zapamwamba Kwambiri Ndi Zofunika kwa GC?
Yankho: Mbale zamphongo zimaperekanso kusindikiza bwino potentha kwambiri, ngakhale zida zina zimathandizira zipewa zotsekemera.

Ndikufuna kudziwaMomwe mungayeretsere mutu chromatography? Dinani apa kuti mufufuzenso

Mapeto

Kusankha HPLC yoyenera ya HPLC kapena GC kumafunikira kuti muganizire zoyeserera, kuyesezedwa kwa chida, komanso zochitika. Pofuna kukhazikitsa zida za vial ndi zosokoneza, mutha kukulitsa kudalirika kwa data ndikuchepetsa ndalama zonse zoyeserera.