Kuchepetsa zotsatira za adsorption mu chromatography (GC)Mbale ndizofunikira kupeza zotsatira zolondola komanso zotheka. Adsorption imatha kubweretsa kuwonongeka kwa zitsanzo, kuipitsidwa, komanso kusiyanasiyana. Blog iyi iyang'ana njira zosiyanasiyana kuti muchepetse izi, poganizira za kusankha, kukonzekera kwa zitsanzo, ndi zida.

Mukufuna kudziwa zambiri chifukwa chifukwa chiyani pali mbale zamatumbo omwe amagwiritsidwa ntchito mu chromatography?Chifukwa chiyani mbale zamtchire ndizogwiritsidwa ntchito mu chromatography? 12

Kuzindikira ADSORRARES MU MIWANDA

Adsorption ndi pamene mamolekyu ophatikizira gasi amatsatira pamwamba pa vial kapena zigawo zake. M'matumba owunikira, osasunthika okonda kuphatikiza (vocs) amasulidwa ku chitsanzo mu gawo la gasi pamwambapa. Komabe, ngati izi zimalumikizana ndi makoma a vial kapena septum, kuchuluka kokhazikika komanso kosagwirizana komanso kunyalanyaza kukhulupirika kwa data kumatha.

Zinthu zazikulu zomwe zimakhudza Adsorption

Kuphatikizidwa kwachuma: Mtundu wa zinthu zomwe zimagwiritsidwa ntchito mu mutu wamutu umakhudza kwambiri adsorption.Mbale zagalasiM'malo mwake, makamaka omwe amapangidwa ndi galasi lazibowo, amakhala ndi zotsekemera zochepetsetsa poyerekeza ndi mbale za pulasitiki. Izi ndichifukwa choti ndiosalala, yosalala imachepetsa kulumikizana ndi zinthu zosasunthika.

Dera la Padziko Lonse: Malo owoneka bwino ku kuchuluka kwa kuchuluka kwa vial amathanso kukhudzana ndi adsorption. Mbale zazing'ono kapena mbale zokhala ndi nsonga zochulukirapo ndi njira yamadzimadzi imatha kuchepetsa mphamvu ya adsorption poletsa kulumikizana kwa mawu.

Kutentha ndi nthawi: Kutentha kowonjezereka kumawonjezera kusanthula kwa kafukufuku, koma amathanso kukulitsa adssorption ngati zinthu za vial sioyenera kutentha kwambiri. Kuphatikiza apo, nthawi yofanananso yofanana ija imatha kulola nthawi yambiri kuti adsorption ichitike.

Ndikufuna kudziwa zambiri za mutu wa mutu, chonde onani nkhaniyi: Maupangiri Okwanira a Mbale: Zosankha, kusankha, mtengo, ndi kugwiritsa ntchito

Njira zochepetsera zotsatira zake

1. Sankhani zamitundu yoyenera

Kusankha Mbale Zapamwamba Kwambiri Kwambiri ndizofunikira kwambiri kuti muchepetse zotsatira za AdSorption. Galasi la Borosiltialillauring ndikulimbikitsidwa chifukwa cha kukana kwake kwa mankhwala ndi kuyanjana kotsika ndi ma voc. Ngati Mbale zapulasitiki zimafunikira, onetsetsani kuti amapangidwa kuchokera ku polyethylene (hdpe) kapena polypropylene, yomwe ili ndi ma adsorption otsika poyerekeza ndi mapulaneti ena.

2. Konzani chitsanzo cha zitsanzo

Kukonzekera bwino koyenera kumatha kuchepetsa kuthekera kwa adsororption:

Vesi la zitsanzo: Onetsetsani kuti makina amadzimadzi amakhala 10-50% ya voliyumu ya vial. Izi zimathandizanso kukhala ndi mutu wokwanira pochepetsa kulumikizana pakati pa madzi ndi makoma a vial.

Kugwiritsa Ntchito Kutaya: Kusiyidwa kumatha kusintha makonzedwe obisika ku mtundu wina asanasanthule asanasanthule, potero kukulira kulowererapo kosasunthika komanso kuchepetsa adsorption. Izi ndizothandiza kwambiri pamankhwala omwe amakonda ku ADSORB kuti ikhale.

Maganizo a matrix: samalani ndi zomwe matrix. Kulemera kwambiri molecular kapena zitsanzo zopepuka kumafunikira nthawi yayitali kapena njira zapadera zochepetsera zotsalira zomwe adsorb kupita pamwamba pa vial.

3. Mikhalidwe Yachilengedwe

Kuyendetsa Zinthu Zachilengedwe Pa nthawi ya makanema kungathandize kuchepetsa adsorption:
Kuwongolera kutentha: khalani ndi kutentha kosalekeza pa kufanana ndi kusanthula. Pewani kutentha kwambiri, zomwe zingakulitse kukakamizidwa kwa mavosi ndikutsogolera kusanja kwachikumbutso kapena kuwonongeka kwa mankhwala osokoneza bongo.

Muchepetse voliyumu yofa: Onetsetsani kuti kulumikizana konse mu dongosolo kumakonzedwa kuti muchepetse voliyumu yofa, pomwe nthenga zimatha kufooka kapena Adsorb musanafike pagombe la GC.

4. Ikani maluso oyenera oyenerera

Njira yosamutsa mutu imatha kukhudza adsorpption:

Gwiritsani ntchito autosambir: Stute Studyspace Shappyr imapereka kukakamizidwa ndi nthawi yoyeserera, kuthandiza kuchepetsa kusiyanasiyana komwe kumachitika chifukwa chogwira mawu.

Makina Okanikiza: Gwiritsani ntchito stert gasilization musanayambe kusinthana ndikuchepetsa chiopsezo cha kuchepa kwa chidule chifukwa cha kuthawavial. Kuchedwa kwakanthawi pambuyo pakuyesa kumalola kusakanikirana bwino kwa mpweya womwe uli ndi vial.

5. Kukonza pafupipafupi ndi kambukidwe

Kusamalira kwa madongosolo a GC

Onani kukhulupirika kwa septum: Onetsetsani kuti septur yomwe imagwiritsidwa ntchito mu vial imagwirizana ndi chitsanzochi ndipo imapereka chisindikizo chabwino chomwe sichingatambazitse zodetsa. Ganizirani pogwiritsa ntchito Septa yokhala ndi PTFF-yolimba ya ortic sol sol.

Kuyang'anira dongosolo la dongosolo: yeretsani mzere wosinthira ndi zigawo za GC pafupipafupi kuti muchepetse kusanthula kwa m'mbuyomu kuti mufotokozere zokongoletsera zosafunikira mu chromatogram.

Mukufuna kudziwa kutsuka mutu chromatography vial? Onani nkhaniyi:Kodi mungayeretsetsetse mutu?

Mapeto

Kuchepetsa zotsatira za AdSorption mkatiMbale za GC mutuKufuna njira yathunthu yomwe imaphatikizapo kusankha zinthu zoyenera, kukonza zitsanzo pokonzekera, kuwongolera nyengo yachilengedwe, ndikukhazikitsa maluso othandiza, komanso mosasunthika. Polankhula ndi zinthu izi, opanga akhoza kusintha kwambiri kudalirika komanso kulondola kwa zotsatira za GC, zomwe zimapangitsa kuti zikhale zodalirika zosiyanasiyana pakuwunikira zachilengedwe.

M'mbuyomu:

1.5ml Mbale zamadzimadzi a chromatography (LC)

Ena:

Momwe mapangidwe a 1.5ml gc amawonjezera njira yokonzekera