Hplc vs. ion chromatography: 4 Key kusiyana kwakatsanangurwa
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Hplc inosiyana sei neIon chromatography? 4 mapoinzi

Sep. 6th, 2024
Yakakwira performance liquid chromatography .

Unoda kuziva mhinduro makumi mashanu nezve HPLC vials, ndapota tarisa chinyorwa ichi:50 Mibvunzo inowanzo bvunzwa paHPLC vials

Kuparadzanisa Mechanism

HPLC inoshandisa hydrophobic kana hydrophilic yekudyidzana yekuparadzaniswa. Inogona kupatsanura huwandu hwakasiyana-siyana hwejekisari makomputa, polar uye isiri-polar, uchishandisa dzakasiyana siyana dzemhando dzechikamu uye organic sol sol sol soles sosi yemhando. Kuparadzanisa kunovimba nehukama hwekufungisisa kwekuongorora kwechikamu chechiteshi, icho chinogona kunyengedzwa kuburikidza neGradient Eleation kuti uwedzere kugadzirisa resolution.

IC, kune rumwe rutivi, kunyanya inoshandisa iyo inoshandisa ion yekutsinhana macaps kupatsanura ionic uye polar compound. Chikamu chomunharaunda chinowanzoumbwa neIon-exchange, kubvumira kuparadzaniswa kwakabudirira kwezvinonhuwira nejasi. IC inoshandisa male eleents, kazhinji inosanganisira mvura yekuwedzera ine mvura yakadzika nemaronda kana acids, uye zvakawanda kuzviparadzanisa zvinoitwa pasina kudiwa kwekuda kweGradient Elek.

Ongorora Type


HPLC iri kuita zvakasiyana uye inogona kubata yakakura spectrum ye organic compounds, ichiita kuti iite kuti zviite zvekushandisa mumishonga yemishonga, chikafu, uye kuongororwa kwezvakatipoteredza. Nekudaro, zvine zvazvisikwa kana zvasvika pakuparadzanisa ionic mhando, dzakadai seinonzwika uye macations, izvo zvinowanzove zvisina kukodzera kuenderana neHPLC makoramu.

IC Izvo zvinonyanya kubatsira muminda yakadai sekutarisa kwezvakatemerwa, kuchengetedzwa kwechikafu, uye kuongororwa kwemifananidzo, uko kweionic makompiso. IC inogona zvakare kuona isiri-ionic polar compound kana iyo yakakodzera iyo column uye nhare phossi.
Marii yamunoziva nezve ongororo yeHPLC uye gadzirira HPLC? Ndokumbirawo kuti muverenge chinyorwa ichi kuti udzidze zvimwe:Ndeupi musiyano pakati pekuongororwa uye gadzirira hplc?

Kuongorora Nzira


HPLC inowanzo shandisa uv Detectors, iyo inoda ongororo yekunyora uv mwenje. Izvi zvinopa zvinonetsa kuona mamwe makomendi, kunyanya izvo zvisingaite UV mwenje, senge mamwe ion uye organic acids.

IC Kazhinji inoshandisa Kuwanikwa Kwakawanikwa, iyo inoshanda kune ionic mhando. Nzira yacho inogona kuwana yakakwirira sensitivity kuburikidza nehunyanzvi semakemikari ekudzora

Zvishandiso

HPLC inozoshandiswa zvakanyanya kuongororwa kweiyo yakaoma organic musanganiswa, kusanganisira mishonga yemishonga, yekuwedzera chikafu, uye masampuzhinji emunharaunda. Kugona kwayo kupatsanura makomisheni akafanana kunoita chishandiso chine simba mumasango akasiyana siyana.

IC inonyanya kubatsira kuti application inoda kuongororwa kweionic mhando, senge mvura yemvura yekuyedza, ongororo yekudya, uye yekuchena kwekuchena kuyedza. Izvo zvinowanzoshandiswa kuenderana neHPLC, kuendesa kugona kweiyo ongororo yemarabhoritari kuti ubatanidze huwandu hweIonic uye polar ongororo.

Mukupfupikisa, sarudzo pakati peHPLC uye iC inoenderana zvakanyanya pane izvo zvinodikanwa zvekuongorora, kusanganisira chimiro chemakomputa chiri kudzidza uye kushushikana uye kugadzirisa kunodiwa. Hplcinokodzera mhando dzakasiyana dze organic compound, nepo ic iri nzira yesarudzo yekuongororwa kweionic uye mhuka dzepolar.

Unoziva sei nhanho dzekugadzirira chromatography vials yekuongorora? Verenga chinyorwa ichi kuti udzidze zvakawanda: Matanho matanhatu kugadzirira chromatography vials yekuongorora
Yapfuura:
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