Mokhoa oa chromatography ke mokhoa oa bohlokoa oa k'hemistri oa senokoaelo se sebelisetsoang ho arohana le ho hloekisa metsoako e tsoang motsoako. Har'a mekhoa e fapaneng e fumanehang, ho na le flash chromatography e sebetsang ea chromatography e telele (HPTC) ke mekhoa e 'meli e phahameng, e' ngoe le e 'ngoe e nang le lits'ebetso tse ikhethang. Sengoliloeng sena se tla potlakela ho kena-kena metheong, lipheo, likoluoa, likokoana-hloko le katleho e akaretsang ea Flash chimatographs vs. Hplc.

U batla ho tseba haholoanyane ka ho itokisa ha samc, ka kopo sheba sengoloa sena:HPTC sampole ea boitokiso ba litokisetso tse ntlehali

1. Molao-motheo oa ts'ebetso

Flash chromatography ke mokhoa oa boitokisetso o sebetsang botlolo bo khabisitsoeng ho feta mohato oa Statery, hangata sil. Mohlala ona o jarollotsoe kholomong, joalo ka leleka li phaphatha, likarolo tse fapaneng tsa motsoako li arotsoe ho latela tšebelisano ea tsona ka karolo ea seteishene. Ts'ebetso ena e potlakile, ka metsela e ntse e hloekisoa metsotsong e seng mekae feela.

Ka lehlakoreng le leng, mokhoa o rarahaneng o boetse o kenyelletsa ho feta sampole ea metsi holima thepa e tlalehiloeng ho Station. Leha ho le joalo, HPTC e sebetsa ka likhatello tse phahameng (ho fihlela ho 4000 psi kapa ho feta) mme hangata e sebelisa boholo ba boemo bo botle ba karohano (3-5 μm). Sena se lumella katleho e phahameng ea karohano le tharollo ho fapana le flash catromatography. Hobane HPLC e ka laola mocheso, khatello le litefiso tsa phallo, e ka fihlela karohano e hlakileng haholo.

2. Lisebelisoa le ho qala

Lisebelisoa tsa mekhoa e 'meli e fapana haholo:

Flash chromatography e hloka ho hlophisoa bo bonolo le pompo ea ho fana ka mohato oa mobile. Ka kakaretso e theko e boima ebile ho bonolo haholo ho sebetsa ho feta tsamaiso ea HPPC.

Litsamaiso tsa HPpc li rarahane haholo, tse nang le lipompo tse nang le bohloa, lipono (joalo ka li-uv tsa UV-le litsamaiso tsa ho fumana data. Hobane lisebelisoa tsa hlcc li hatela pele ka mokhoa o letšo, matsete a eona a pele a feta.

3. Lebelo le katleho

E 'ngoe ea liphapang tse kholo ka ho fetisisa pakeng tsa chromatography le hplc ke lebelo la bona:

Flash chromatography e ka tšoaea mekhapo e potlakileng, hangata e qeta likarohano nakong ea metsotso e 30. Lebelo lena le bohlokoa haholo ho litsebi tsa k'hemik'hale tse manyolo tse hlokang ho arola lihlahisoa tsa Real Reut.

Ha Hplc e sebetsa hantle haholo ho latela boleng ba karohano, hangata e nka nako e telele ho phethela mesebetsi e ts'oanang ka lebaka la tlhaho le ea ho arohana hangata.

4. Boea le qeto

Flash chromatography e sebetsa haholo ho tlhoekiso ea mahareng, empa e kanna ea se ke ea fana ka maemo a hloekileng a bohloeki bo hlokahalang bakeng sa tlhahlobo ea ho qetela ea sehlahisoa. Ka kakaretso e ka fihlela maemo a hloekileng a bohloeki, empa a ka se beletsoe motsoako o rarahaneng haholo moo qeto e phahameng o ka tšoaeang liphoso.

HPLC e hlahisa ka ho fana ka metsoako e phahameng ea bohloeki ka lebaka la bokhoni ba eona ba maemo a arohaneng hantle. Hangata ke mokhoa oa boikhethelo ha o sebetsana le motsoako o rarahaneng kapa ha likarohano tse phahameng li hlokahala.

5. Mohlala oa mohlala

Flash chromatography e lumellang mekhahlelo e meholo e kholo ho feta hplc. Bokhopo bona bo etsa hore e be e loketseng ho hloesetsa metsoako e mengata, e leng molemo nakong ea methati ea pele ea maikutlo.

HPTC, ha e khona ho sebetsana le boholo ba sampole e fapaneng, ka kakaretso e na le litheko tse tlase ka lebaka la boholo ba eona bo sootho le litlhokahalo tse phahameng tsa khatello.

6. Mehopolo ea theko

Litšenyehelo ke karolo ea bohlokoa ha u khetha pakeng tsa flash chromatography le hplc:

Flash chromatography ha e theko e tlase, ka bobeli mabapi le lisebelisoa le litšenyehelo tsa ts'ebetso. Ho bonolo ho theha 'me ho hloka tlhokomelo e fokolang, e e khahlang ho laboratories e nang le lipalesa tse fokolang.

Le hoja Hplc e na le qeto e ntle le bohloeki bo botle, e na le litšenyehelo tse ngata tse sebetsang ka lebaka la ho rarahana ha sistimi e ngata e le likholomo le lilallo.

7. Likopo

Mekhoa eo ka bobeli e na le ts'ebeliso e fapaneng masimong a fapaneng:

Flash chromatography e sebelisoa haholo ka har'a li-syntthesis tse phelang bakeng sa tlhophiso e potlakileng ea lihlahisoa tsa litlhahiso. E ratoa haholo libakeng tsa thuto moo karohano e potlakileng ea mekhahlelo e hlokahalang mererong ea lipatlisiso.

HPTC e sebelisoa haholo tšimong ea meriana ea meriana ea litlhahiso tsa litlhahlobo tsa litlhare, lithibelo ka tlhahlobo ea polokeho ea lijo, le lithuto tse entsoeng ka tefo moo litlhokahalo tse nepahetseng lia hlokahala.

Melemo le mathata

Flash chromatography

Melemo: nako e potlakileng ea ho reka. Lisebelisoa tse tlase le litšenyehelo tsa ts'ebetso. Sethala sa Setup ea Boxerr le Setsebi se sa Hloleheng. Sampole e phahameng.

Likotsi: Repaesekela e tlase ha e bapisoa le HPTC. E kanna ea se fihlelle maemo a phahameng a bohloeki a hlokahalang bakeng sa tlhahlobo ea ho qetela ea sehlahisoa. E se e sebetse hantle bakeng sa motsoako o rarahaneng o hlokang karohano e ntle.

Ts'ebetso e phahameng ea ts'ebetso ea li-chromatography

Melemo: Phello e phahameng le maemo a hloekileng a bohloeki. Bokhoni ba ho sekaseka hantle motsoako o rarahaneng. Taolo e nepahetseng ea maemo a ts'ebetso e ntlafatsang hape.

Litsietsi: Nako ea ho sebetsa butle butle. Litsela tse phahameng tsa ho tsetela le tse sebetsang. Lisebelisoa tse rarahaneng haholoanyane li hloka koetliso e khethehileng.

Ka kakaretso, flash chromatography le hplc e mong le e mong o na le melemo le likotsi ebile li loketse likopo tse fapaneng tsa k'hemistri. Flash chromatography e potlakile ebile e theko e tlase, e e etsa e loketseng mesebetsi e potlakileng ea tlhoekiso ea manyolo. Ho fapana le moo, HPLC e na le qeto e ntle le bohloeki bo botle, e hlokahalang bakeng sa mosebetsi oa Tlhahlobo, mahlale a tikoloho le tlhahlobo ea polokeho ea lijo.

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Qetellong, khetho pakeng tsa flash chromatography le hplc e ipapisitse le litlhoko tse itseng tsa laboratori, ho kenyelletsa le bophahamo ba boruti, litekanyetso tsa lichelete le nako. Ho utloisisa liphapang tsena ho lumella bafuputsi ba ho khetha theknoloji e loketseng, e netefatsa litholoana tse ntle ka tlhahlobo ea bona ea li-chrotographic.

E fetileng:

U ka khetha septa e nepahetseng joang bakeng sa solvent ea hau?

E 'ngoe:

HPTC e fapane joang le kholomo ea GC?