Ho nepahala ha k'hemistri ea tlhahlobo ea tlhaiso-leseling ke ea bohlokoa haholo, ka ho nepahala ho nepahetseng ha ho buuoa ka mokhoa o nepahetseng oa ho bua ka bongata ba mohlalaLithako tsa Chromatograft. Ho sa tsotelehe ho sebelisa li-chromatograph tsa metsi tse phahameng haholo (HPLC) kapa li-chromatography tsa "chromatography (GC), ho fokotsa sampole Mona ke tataiso e felletseng ea hore na u ka hlasela teka-tekano ena joang.

1. Fana ka mekhoa ea ente:

Ho sebelisa mekhoa e kang ho arola ente ho GC le ho tlatsa ho ho qeta HPLC e ka fokotsa sampole e ea hlokahala, ha u ntse u boloka botšepehi ba mantlha ha u ntse u le mehlala ea bohlokoa.

2. Pripise pisting:

Mahlale a sejoale-joale a lipehelo tsa mehleng ena a nang le masene a phomolo a bolokiloeng a etsa hore ho be le tahlehelo e fokolang nakong ea phetisetso le mokhoa o nepahetseng oa ho fana ka mangolo a microscale.

3. Mekhoa ea mahloriso:

Moo ho khonehang, khatello ea maikutlo e lokela ho etsahala pele ho ente ea sampole ea hau. Mekhoa ea ho fetoloa kapa ea fetohang ho ba motsamao e ka u thusang ho fumana mahloriso a phahameng ebe u netefatsa litlhahlobo tse nepahetseng ka mantsoe a mangata a mohlala.

4. Fokotsa molumo o shoeleng:

Khetha li-vaerise le lits'oants'o tse nang le molumo o fokolang oa ho thibela tahlehelo ea mohlala ka lebaka la ho ba le moea. Nahana ka lintho tse kangLikokoana-hlokokapaLi-microvolume li-vialsE le tharollo e ka khonehang.

Lekola leseli-leseling ka botebo mabapi le litlolo tsa Hplc tse Vial. Fumana litaba tsa bohlokoa le tsebo ka taba ena ea bohlokoa: HPTC Vial Intrys: Elisa ho nepahala le ho ba le botshepehi

5. Sebelisa likhama tsa microscale:

Liphalletsi tsa microscale li etsoa ka mokhoa o ikhethileng ho sebetsana le bophahamo ba mohlala o fokolang ntle le ho bolaoa ho feteletseng, ho tsamaisana le mekhoa e mengata ea tlhahlobo ea morao-rao.

6. Ho ts'oaroa ka nepo:

Le ha ho thunngoa ha majalefa ho ka utloahala ho teneha, ho ka thusa ho tsamaisa litlhahlobo tse nepahetseng. Empa sena se tlameha ho etsoa ka hloko ho qoba ho nyaloa hape le liphetho tse se nang meeli.

7. Liphello tsa sejoale-joale tsa sejoale-joale:

Baahi ba kajeno ba hopole ba qala ho ba le mahlale a feteletseng ba nang le ente e hlakileng haholo, e ka tsamaisoang haholo molemong oa mohlala. Ho sebelisa likarolo tse joalo ho tla thusa boiteko ba hau.

8. Ts'ebetso ea Kholomo:

Likholoma tse bolokiloeng hantle tse sebetsang hantle ho Pholoso ea Peak li ka ntlafatsa karohano, tlhahlobo e kenang ea molumo o monyane oa mohlala ntle le ho sekisetsa boleng ba data.

9. Fokotsa boemo ba mohlala oa mohlala:

Likarolo tse tsoang ho 'mele oa boholo bo sa sebetseng li ka etsa litholoana tse matla, kahoo u etse mekhoa e nepahetseng ea LaB le ho sebetsana le likhahla tsa leoatle le ho sebetsana le litšila ka hloko ho fokotsa tšilafalo.

10. Netefatso le Calibration:

Khafetsa o netefatsa lisebelisoa tsa hau khafetsa ho netefatsa hore mekhoa ea ho fokotsa molumo e hlahisa litholoana tse nepahetseng.

Ka motjeko o rarahaneng oa chromatography, e leka-lekana lipakeng tsa ho nepahala ebile ho boloka disehlala ea bohlokoa ho ka fihlella ka litloaelo tse nahanang. Ha ba sebelisa mekhoa e hloahloa ea ho ithiba, theknoloji e tsoetseng pele le mokhoa oa boithati, ba ka qobella mahlaseli a hau ho lithaba tse ncha. Ha sechaba sa saense se ntse se tsoela pele ho sutumelletsa meeli ea se khonang, tsebo ea ho fokotsa seluloana sa litlolo ea chromatography e fetoha tsebo ea bohlokoa bakeng sa lipatlisiso tse nepahetseng le tse sebetsang.

Fumana leseli le felletseng ka ho fetisisa lipotsong tse holimo tse 50 tse botsoang khafetsa ka li-pilc vaianos tse sehloohong sena se rutang. Bula Likarabo tseo u li hlokang: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Ma-Delec Mehato e leqepheng la 16

E 'ngoe:

Ke khetha septum e nepahetseng joang bakeng sa alpl ea ka ea HPLC?