U ka thibela li-bubble tsa moea joang ha u sebelisa syringe lifilimi? Mehato e 6

Lifilimi tsa Syringeke lisebelisoa tsa bohlokoa tikolohong ea laboratori 'me li sebelisoa haholo bakeng sa ho hloekisoa sampole, thipa le linako tse fapaneng tsa lits'ebeliso. Leha ho le joalo, phephetso e tloaelehileng ea phepo e tloaelehileng e thulana le ho hloka mohala ke ho thehwa ha li-bubble. Li-bubble tsena li ka senya phallo ea sampole, e lebisang ho liphetho tse sa nepahalang kapa esita le tšilafalo. Blog ena e tla fana ka tataiso e felletseng ea ho thibela li-bubble ha u sebelisa fildingse litlolo tse sebetsang hantle le tse tšepahalang.

Batla ho tseba tsebo e felletseng ka syringe filter, ka kopo sheba sengoloa sena:Tataiso e Phethahetseng ea Syringe Fillies: Likahaka, khetho, theko le ts'ebeliso

Ho utloisisa lisosa tsa li-bubble tsa moea

Bubble ea moea e ka theha nakong ea ho hloka chelete ka mabaka a 'maloa:

Ho ne ho sa tšoanelehe ho Pholoha: Haeba sefahleho sa syring se sa rutoang pele se sebelisoa, moea o ka ts'oaroa ka har'a filthara kapa syringe ka boeona kapa siringa.

Litefiso tse potlakileng: Ho chesetsa ho sefa ka potlako ho ka baka mofere-fere, o ka tsebisa moea tsamaiso tsamaisong.

Mohlala o sa Fellong oa Mohlala: Ho etsa lintho kapa ho thothomela mohlala ka mokhoa o matla ho hlahisa moea ka har'a syringe.

Sampole ea viscous: sampole e teteaneng e akheloa ho feta motheong oa bubble ka lebaka la thepa ea tsona ea phallo.

Ho utloisisa lisosa tsena ho bohlokoa ho kenya tšebetsong leano le sebetsang la ho thibela bubble.

Metsoishene e 6 ea ho thibela li-bubble tsa moea

1. Priming Syrits

Priming ke e 'ngoe ea mehato e nyarosang ka ho fetisisa ea ho thibela li-bubble tsa moea:

Mokoallo Membrane: Pele a qala ho qala, ho taka tharollo ea ho hlapanya ka syringe le ho hokela filthara. Tobetsa konopo ho leleka moea o mong le o mong o tsoang ho tsoa ho filthara. Mohato ona o tiisa hore lifate tsa moea li kolobisoa ka botlalo ebile li fokotsa menyetla ea li-bubble tsa moea tse entsoeng nakong ea ho tima filotch.

Fydrophomcic Filters: Bakeng saMembranes ea hydrophobic,Ha PTF, ho bohlokoa ho li shebella ka solvent e loketseng pele a sefa disam ea aquere. Sena se thusa ho eketsa ho nepahala le ho thibela li-blockge tse ka setisang moea.

Batla ho tseba haholoanyane ka li-file tse eketsehileng tsa 0.22, ka kopo sheba sengoloa sena:Tataiso e felletseng ho li-filthara tse 0,22 Micron Micron: Ntho e ngoe le e ngoe eo u e hlokang ho e tseba

2. Sekhahla sa Fistation

Ho boloka sekhahla sa lifaele se liehang le se laoloang se bohlokoa:

Etsa kopo khatello ea bonolo: Etsa kopo khatello e tsitsitseng, e bonolo ea syringe frunger ea syringe ho laola sekhahla sa ho phalla. Ho itšehla thajana kapa ho matlafatsoa ho ka baka mofere-fere 'me u hlahise moea tsamaisong.

Size the Syringe: For viscous samples, consider using a larger syringe to achieve smoother flow dynamics without applying excessive pressure that can cause bubble formation.

3. Tšoara sampole ea hau ka hloko

Mekhoa e metle ea ho sebetsana le eona e ka fokotsa ho imena ea moea haholo:

Kenya 'mino oa syringe: ha a kenya sampole hosyringe, etsa bonnete ba hore nale kapa ntlha e qotsoa ka botlalo mokelikeli ho thibela ho ingolisa moeeng.

Qoba ho kuta ka ho feteletseng: Sebakeng sa ho kopanya sampole kapa ka matla ho kopanya sampole, le sephara kapa ho koala siminga ho kopanya moea o mongata. Motheo ona o sebetsa bakeng sa mehlala eohle ea sampole, haholo maikutlo a Viscous kapa disalpole tse holofetseng.

4. Litaba tsa liketsahalo tsa Viscosity

Lisamona tsa Viscous li na le maikutlo a hona joale a ikhethang mabapi le sebopeho sa bubble:

Pregaritation: Nahana ka sampole ea lifate tsa litšoantšo tse fallisang pele u sebelisa sefahleho se nang le boholo bo boholo kapa sengoloang ho tlosa likaroloana tse kholo le ho fokotsa pono. Bohato bona bo ntlafatsa matla a phallang mme o fokotsa sebopeho sa bubble nakong ea firatration e latelang.

Sebelisa filthara e nepahetseng: Khetha sefako se nang le boholo bo nepahetseng bo ipapisitse le mofuta oa sampole. Bakeng sa likaroloana tse nyane, boholo bo fokolang bo hlokahalang; Bakeng sa litharollo tsa Viscous, ligiter tse hlahelletseng bakeng sa pono e phahameng li ka thusa ho boloka phallo ntle le ho koala.

5. Tlhoekiso ea moea pele e fifala

Pele o hokahanya syringe ho filthara, e nyatsa ho leleka moea o mong le o mong o ts'oaretsoeng ka syringe:

Ho phetla moea o tsoang syringe: Pele u fumana filithara ea syringe, tobetsa plunger ho fihlela mokelikeli o tlala moea o mong le o mong o tlalo. Ketso ena e tiisa hore mokelikeli o phalla hantle ka sefahlehong ntle le tšitiso ea bubble.

6. Khetha bokhoni bo nepahetseng ba syringe

Boleng ba syringe bo ka ama ts'ebetso ea filthara:

Khetha boholo bo nepahetseng: Ho sebelisa syringe ho nolofalletsa ho boloka boemo bo matla nakong ea khatello ea khatello nakong ea ho fana. Syringe e kholo haholo e ka baka khatello e feteletseng, ha syringe e nyane haholo e ka hloka ho fihlela e ntse e tsoela pele.

Mekhoa e metle ea ho fifala ho hongata

Ho ntlafatsa ts'ebetso ea ho foka le ho fokotsa li-bubble:

Lekola botšepehi ba filthara khafetsa: Etsa bonnete ba hore Filtharaha e senyehe le ka har'a letsatsi la eona la ho felloa ke nako pele u sebelisoa. Setsofe se senyehileng se tla fella ka ho fifala le ho eketsa kotsi ea sebopeho sa bubble.

Ho hlokomela maemo a tikoloho: Lintho tse kang mocheso le mongobo li ka ama mohlala oa mohlala oa mohlala le sebopeho sa bubble. Etsa liteko tikolohong e laoloang teng ha ho khoneha.

Rekoto ea ho rekota mekhoa ea ho fana ka litlatsetso: Boloka litlaleho tse qaqileng tsa mekhoa ea hau ea lifilimi, ho kenyelletsa le litaba life tse kopaneng tse amanang le sebopeho sa bubble. Lirekoto tsena li ka thusa ho tseba lipaterone le ho ntlafatsa lits'ebetso tsa nakong e tlang.

Batla ho tseba haholoanyane ka li-file tse eketsehileng tsa 0.45 micron, ka kopo sheba sengoloa sena: Tataiso e felletseng ho 0.45 Micron Micron Micron Micron: Ntho e 'ngoe le e' ngoe eo u e hlokang ho tseba

Sephetho

Ho thibela pōpo ea bubble ha e sebelisoaLifilimi tsa Syringe Ho bohlokoa ho fihlela liphetho tse nepahetseng le tse tšepahalang tse ka hlahlojoang ea laboratori. Ka ho sebelisa mekhoa e nepahetseng ea PriS ea pele, ho laola litekanyetso tsa ho fana, ho sebetsana le litaba tsa lits'oants'o, le ho khetha moea o nepahetseng oa li-syringe.

Ho kenya tšebetsong maano ana a ka se ke oa ntlafatsa ts'ebetso ea ho hlaka, empa hape e ntlafatsa boleng ba data ka kakaretso ho tsoa tlhahlobong ea tikoloho. Ka ho utloisisa haholoanyane le ho latela mekhoa e metle, li-laboratories li ka ntlafatsa litšila tsa syringe le ho netefatsa litholoana tse atlehileng tsa syrite.