Mekhoa e tloaelehileng ea mohlala ea GC-MS

Khase ea khase ea khase ea chromatographs-boima Ho latela mofuta oa sampole le litlhahlobo tse fapaneng, mekhoa e fapaneng e ka sebelisoa ho lokisa sampole ka katleho. Tse latelang ke mekhoa e tloaelehileng e sebelisoang ho lokisa mehlala eaGC-MS Tlhahlobo ea GC-MS:

Batla ho tseba haholoanyane ka phapang lipakeng tsa LC-MS le GC-MS, ka kopo sheba sengoloa sena:Phapang ke efe lipakeng tsa LC-MS le GC-MS?

1. Lisebelisoa tsa moeli tsa metsi

Bolulo: Mehlala ea metsi ka tloaelo e hlakotsoe ka tekanyo e tlase ea ho pheha hoo e joalo ka Methanol, Acestoromethane ho fihlela 0,1 ho isa ho 1 mg \ ml. Sena se tiisa hore sampole e lumellana le sistimi ea GC mme e fokotsa kotsi ea ho koala sehlekehleke sena.

Tokiso: Pele ho tlhahlobo, sampole e lokela ho se hlotsoe ho tlosa likaroloana tse ka u sitisang ho hlahlojoa. A0.22 μm filtharahangata e sebelisoa.

Tencheng: Bakeng sa disampole tse ka bang le li-soids, tse bokelloang li ka thusa ho arola mokelikeli ho tsoa boitsebiso boo u bo ratang pele bo akheha.

2. Litokisetso tsa sampole tse tiileng

Dissolbletion: Mehlala e tiileng e tlameha ho qhibiliha ka ho hlakoloa e loketseng ea ho pheha. Kenya chelete e nyane (lijo-thollo tse 'maloa) tsa tse tiileng tsa ho sotla le ho kopanya makhetlo a' maloa ho netefatsa khefulo e felletseng.

Ngativatization: Bakeng sa metsoako e sa fetoheng kapa ea polar, matlafatso e kanna ea thusa ho ntlafatsa tsebo le ho ntlafatsa kutloisiso ea ho lemoha. Sena se kenyelletsa ho fetola tlhahlobo ea lik'hemik'hente ho etsa hore e be molemo ho fumana tlhahlobo ea GC.

3. Tlhahlobo ea lihlooho

Starisspace ea Staricspace: Ho ts'oaroa ka matla ho na le sampole e tsitsitseng ho lumella metsoako e sa feleng ea ho fallela hloohong ka holim'a sampole. Hang ha ho leka-lekana ho fihletsoe, lihlooho tsena li ka amoheloa bakeng sa ho sekaseka ho sebelisa syringe ea khase.

Matla a matla a matla (brage le leraba) Mokhoa ona o eketsa maikutlo haholo ka ho tsepamisa mohopolo pele ho tlhahlobo.

U batla ho tseba haholoanyane ka lebaka leo li-vialspace tse sebelisitsoeng ho chromatography ?, Ka kopo sheba liaparo tsena: Hobaneng ha Headspace e sebelisoa li-chromatography? Li-angles

4. Mekhoa ea ho tlosoa

Karolo e tiileng ea Microwexentraction (SPME): Spime) e sebelisa fiber e koahetsoeng ka motsamao o ntšitsoeng ho amohela sekaseka ho sekaseka ho metsi kapa khase. Mokhoa ona o fa motho ea tobileng ka kotloloho ntle le tlhoko ea li-solvent mme e na le thuso haholoholo metsoako e sa tsotelleng.

Ho khesoa ka metsi (ly) le ho khelosa ka thata: Mekhoa ena e sebelisetsoa ho hloekisa mehlala ka likarolo tse rarahaneng pele ho tlhahlobo ea GC-MS.

5. Malebela a ho tsepamisa maikutlo

Nittrogen purging: Mokhoa ona o sebelisoa ho tsepamisa mohopolo ka ho fetolela li-solvent tlasa molala oa naetrojene, o thusang ho sekaseka sekgure.

Mohlala oa boitokiso ba mohlala

Etsa bonnete ba hore li-solvents tsohle tse sebelisitsoeng li sa fetohe li bile li loketse GC-Ms; Metsi a metsi le linollo tse seng-tlaase li lokela ho qojoa.

Sampole ha ea lokela ho ba le li-acid tse matla, mekotla, letsoai kapa likepe tse ling tse ka lematsang kholomo kapa li kena-kenana le tlhahlobo ea GC.

Sampole ea ho qetela e lokela ho ba e se nang likaroloana ebile e lokiselitsoe ka nepo hoLi-vies tsa khalase Ho thibela ho pepeta thepa ho tsoa polasetiki.

Bakeng sa tlhaiso-leseling e batsi ka li-viels tsa AutoSompler bakeng sa li-chromatography tsa khase chromatography, sheba sehlooho sena: 2 ml autosampler

Sephetho

Mekhoa e sebetsang ea mohlala e bohlokoa bakeng sa tlhahlobo e atlehileng ea GC-MS. Mokhoa o mong le o mong o na le melemo e ngoe le e 'ngoe e nang le likopo tse ikhethang ho latela mofuta oa sampole le komporo ea sepheo. Ka ho sebelisa mekhoa ena, bahlahlobi ba ka ntlafatsa ho nepahala, ka lebaka la ho fumana data ea bona e tšepahalang masimong a tikoloho le merima.