Na ua tseba ka likhatiso tsa chromatography?

Mokhoa oa li-chromatography o sebelisoang haholo oa tlhahlobo o sebelisoang le ho supa metsoako ea tsona e fapaneng bakeng sa mohato oa hae o fapaneng bakeng sa mohato oa hae (pampiri). E na le thuso e khethehileng ho k'hemistri le biochemist ha e hlahloba lintho tse ngata tse kang mebala, lia atino acid le metsoako e meng ea tlhaho.

Li-chromatography tsa pampiri li joang?

Li-chromatography tsa pampiri ke mofuta oa li-chromatograph tsa chromatography tsa sefate sa li-chromamaties tsa ho ema. Mokhoa o itšetleha ka karohano e fapaneng ea metsoako pakeng tsa mohato oa boleng (pampiri ea filthara) le karolo ea mobile (solvent). As the solvent moves up the filter paper by capillary action, it carries the components of the mixture at different rates based on their affinity for the two phases.

Melao-motheo ea bohlokoa ea lihlooho tsa chromatography e ka utloisisoa ka likhopolo tse peli tsa bohlokoa: Karolo ea karohano ea chromatography le ho adserpption chromatography.

Karolo ea karohano ea chromatography: Mofuta ona, linthong tsena li tsamaisoa lipakeng tsa mekhahlelo e 'meli ea metsi a metsi. Mokhahlelo oa seteishene o na le metsi ka har'a mekotla ea pampiri, ha mohato oa mobile ke solvent e tsamaeang lipere. Karohano e etsahala hobane metsoako e fapaneng e na le libaka tse fapaneng bakeng sa li-deteary tsa boemo bo phahameng le mobile.

Adsedoppment Chromatography: Thepa e tiileng ea pampiri e sebetsa e le mohato oa setute le metsi a boima. Likarolo tsa motsoako li arotsoe ho latela ho rema ha tsona sebakeng sa bona se tiileng le sello sa bona ka karolo ea metsi.

Likarolo

Mokhahlelo oa Etete: pampiri ea li-chromatographic, hangata e entsoeng ka cellulose. E fana ka sebaka sa likarolo ho latela.

Mohala oa Mobile: Solvent kapa motsoako oa li-solvent tse tsamaeang pampiring ka ketso ea capillary. Khetho ea Solvent e ka ama ts'ebetso ea karohano haholo.

Mohlala: Motsoako o tla hlahlojoa, hangata o bonahatsoa sebakeng se qapiloeng pampiring.

U batla ho tseba haholoanyane ka ho itokisa ha samc, ka kopo sheba sengoloa sena: HPTC sampole ea boitokiso ba litokisetso tse ntlehali

Ts'ebetso ea Li-chromatography tsa pampiri

Ho itokisetsa: Khetha pampiri ea boleng bo holimo e nang le tšekamelo e itseng ho netefatsa karohano e atlehang. Etsa kopo karolo e nyane ea motsoako o motsoako ho setshelo ea pampiri ea chromatography.

Boitokisetso ba mohlala: qhala mongobo oa mohlala ka bokhabane bo loketseng karolo ea mobile.

Ho bona: Sebelisa "milleripette kapa micropipette ho lahlela tharollo e nyane ea sampole ka motheong oa filthara.

Nts'etsopele: Qolla moeli o tlase oa pampiri ea liteko ho fihlela Solvent ea Ntlafatso (Movie Mobile). Joalokaha ketso e nkang matla, lefats'e la qhomarela pampiring ea liteko, le nkile karolo ea mohlala le eona.

Karohano: Ha e le motsamao o fapaneng, likarolo tse fapaneng tsa sampole li tla tsamaea ka litekanyetso tse fapaneng ka lebaka la tšebelisano ea bona le sekhahla se fapaneng ka har'a mobile.

Pono: Kamora hore solvent e se e tsamaile sebaka se lekaneng, pampiri e tlositsoe ho softivent le ho tloha ho omella. Likarolo li ka bonoa u sebelisa leseli la UV, re lik'nyemi tsa lik'hemik'hale, kapa ka ho shebella liphetoho tsa mebala.

Tlhahlobo: Sebaka se tsamaeang le karolo e 'ngoe le e' ngoe se ikemetseng ka pele e lekantsoe ho bala boleng ba RF (sepheo sa ho phomola) bakeng sa tlhahlobo.

Likopo tsa li-chromatography tsa pampiri

Lipampiri tsa chromatography li na le likopo tse ngata masimong a fapaneng:

BOKOBMMMMMMMMMMMMMMIBRY: Tlhahlobo ea Amino acid, tsoekere le li-biomecules tse ling.

Merimaceticalical: tsebiso ea metsoako e mafolofolo le litšila tsa litlhare tsa lithethefatsi. Sebelisoa ho supa metsoako e sebetsang le litšila tsa litlhare tsa litlhare nakong ea taolo le taolo ea boleng.

Saense ea tikoloho: Li-chromatography tsa tikoloho li sebelisoa ho bona li-sinples tsa methapo ea metsi le sekaseka litšila tsa metsi.

Indasteri ea lijo: Mokhoa ona o thusa ho hlahisa litlatsetso, lisele le lihlopha tsa lijo ho netefatsa hore melao ea polokeho.

Melemo ea likhatiso tsa chromatography

Molemo o theko e boima: E hloka lisebelisoa tse fokolang le lisebelisoa li bapisoang le mekhoa e meng ea li-chromatographic e kang hplc (e phahameng ea ts'ebetso ea chromatograph ea cpromatographs). Tsela e bonolo: Ts'ebetso e bonolo haholo ebile e ka etsoa ka lisebelisoa tsa mantlha tsa laboratoring.

Tlhokahalo e tlase ea sampole: Ke mohlala o fokolang feela oa mohlala o hlokahalang bakeng sa ho hlahlobisisa, ho etsa hore o be le sehopotso sa maikutlo a bohlokoa kapa a fokolang.

Ho fapanyetsana maikutlo: E ka arola lintho tse ngata tse fapaneng ho latela thepa ea lik'hemik'hale, ho kenyeletsoa le metsoako ea polar le e seng polar

Meeling ea li-chromatograph tsa chromatography

Leha e le Melemo ea Hae, Li-Cormatography tsa COROMATOS li na le meeli:

Matla a lekanyelitsoeng: Ka kakaretso e loketse lintlha tse nyane tsa mohlala.

Qeto: Ho ka etsahala hore re se ke ra ba holimo joaloka tse fihletseng ka mekhoa e tsoetseng pele e kang hplc kapa khase ea khase ea khase (gc).

Nako ea ho ja: Ka lebaka la sekhahla se butle butle, ts'ebetso e kanna ea nka nako e ntse e bapisoa le mekhoa e meng ea chromatographic.

Tlhahlobo e lekanyelitsoeng: Le ha liphello tsa tšoaetso li ka fumanoa habonolo, mekhoa e meng ea calibration e kanna ea hlokahala ho ntlafatsoa.

Pampiri ea chromatography e lula e le mokhoa oa bohlokoa bakeng sa ho arola le ho sekaseka motsoako ho mefuta e mengata ea saense. Boikutlo ba eona, katleho ea theko ea eona le bokhoni ba ho fana ka liphetho tsa bohlokoa li etsa hore e be sesebelisoa sa bohlokoa bakeng sa litsebi tsa lik'hemik'hale le bafuputsi. Leha e ne e le mefokolo ea eona e bapisoang le mekhoa e tsoetseng pele ea chromatograph, e ntse e sebelisetsoa ka bongata molemong oa thuto le tlhahlobo ea pele-pele ho laboratories.

Batla ho tseba likarabo tse 50 mabapi le li-valc vials, ka kopo sheba sengoloa sena: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang