U ka khetha lipale tsa HPLC

Ka tlhahlobo ea li-chromatagraph, hangata ba tobana le bothata ba ho khetha liteko. Ho sebelisa mohlala o fosahetseng ho ka baka liphetho tse mpe. E kanna ea senya mohlala kapa li-chromatograph.

Ho na le mefuta e mengata e metle ea mohlala, re lokela ho khethile joang? Sengoliloeng sena se tla u bontša mokhoa oa ho khetha li-vials. Ho etsa joalo ho tla ntlafatsa ho nepahala ha hau ho tlhahlobo ea li-chromaticphic.

Ho khetha semelo se nepahetseng sa autosampler ke oa bohlokoa bakeng sa ho netefatsa ho nepahala le ho ts'epahala ha sephetho sa hau sa tlhahlobo:Lintlha tse 5 li hloka ho nahana ha u khetha ho senyeha ho sa sebetseng.

Mefuta ea li-piplc tsa HPLC

Re ka arola sampole ea sempole ka boitsebiso ba bona, molomo oa bona le sebopeho sa kahare. Mefuta e fapaneng ea li-vaespole tsa sampole e loketse liteko tse fapaneng.

Lintho tse bonahalang
Pele re utloisisa boitsebiso boo, ha re utloisise ka ho hlaka ka mokhoa o hlakileng oa ho thibela ka mokhoa o hlakileng oa mohala. City e ntle ea tefo ke phetoho ea bolelele bakeng sa tekanyo e 'ngoe le e' ngoe ea mocheso. Kolobetso e tlase e sa sebetseng e bolela hore khalase e ka mamella phetoho ea mocheso. Ka mantsoe a mang, ke: bokhoni ba khalase ho emisa liphetoho tse mahlonoko. USP (United States Pharmacope)) e etsa hore khalase ea khalase ea lab. E ipapisitse le ho hanyetsa ka metsing.

Re tseba ka kotloloho e ke keng ea lekanngoa ea khalase. Kahoo, khetho ea boitsebiso bakeng sa litumelo tsa mohlala ho bonolo.

1. Mofuta oa USP 1, Class Bula khalase ea 33 e le khalase ea lik'hemik'hale. E sebelisoa haholo ka li-laboratori, haholoholo bakeng sa chromatography. Sehlopha I chris se entsoe haholo ka silicon le oksijene, ka tekanyo e matla ea boron le sodium. E na le sllubility e tlase le ka mokhoa o hlakileng oa ho nka khato ea 33.

2. Mofuta oa USP 1, sehlopha sa B 51 boholo ba silicon le oksijene. E na le lesapo le fokolang la boron, sodium le ho feta tsa Alkali tse ngata ho feta khalase. Leha ho le joalo, e ntse e ka kopana le litlhoko tsa laboratori. Ha li lekane satoloso ea mela ke 51.

3. Khalase e khabisitsoeng kapa e matla ke mofuta o le mong oa khalase e borolicate. Ho entsoe ka hare ho kotanta ka ho hohela bokaholimo ba eona ka lisitilase tsa manyolo. Hydrophobic le Inert ke bokaholimo ba khalase. E sebetsa bakeng sa ho boloka mohlala oa nako ea nako e telele, metsoako e metle ea PH-ENNORES, LE HO BONAHALA.

4. Mofuta oa USP le NP Sodium-calcium ea NP Ha ba hane ha ba hanyetsanang ho feta khalase ea borolicate.

5.Polypropylene (PP).

Mofuta oa molomo oa Bottle
1. Mouthmin Mouth 2. Bayonet Mouth 3. Molomo oa Thread

'Mala oa' mele oa li-vials
Hlakisa kapa amber

Mehopolo ea ho khetha li-valpc tsa HPLCC

Ho ikamahanya le maemo
Kamora ho utloisisa boitsebiso ba masela a khalase ea khalase, re tla bua ka mokhoa oa eona oa ho ikamahanya le maemo.

1. Sebelisa sampole e sootho bakeng sa mehlala e bobebe bakeng sa mehlala e bobebe. Mehlala e sootho ka li-vaes e ka senya leseli le ho thibela lifoto.

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3. Bakeng sa ho khetholla sampole ea mohlala, sebelisa li-tubes tse ka hare. U ka sebelisa li-tubente tse tsitsitseng tsa kahare, kapaLintlha tse phahameng tsa mohlala. Tsena ke tsa disampole tse tloaelehileng ka mekhahlelo e nyane.

4. Ka mokhoa oa IIC, Sebelisa mohlala oa mohlala o entsoeng ka polypropingne (PP), eseng mohlala oa mohlala oa kgara.

Khetha ho latela molumo oa mohlala

Ho bona disampole ea Trace, re hloka ho sebelisa li-piefuls tse nyane. Ba etsa hore lekhetho la tlhahlobo le nepahala. Tse latelang ke litakatso tse ngata ke tsebiso e loketseng mehlala ea litlhaloso tse fapaneng.

1. Mohlala oa mohlala o ka tlase ho 2ml
Li-vavers li tla mefuta e mengata.Ho na le li-vavers tse microsteample (15μl-800μl) le sampole tse phahameng tsa ho hlasimolla (30μl-1.5ml). Ho boetse ho na le li-tubes tsa kahare (100μl-400μl). Ho boetse ho na le li-valyproplene micramplene micramplene. Ho boetse ho na le li-viels tsa mohlala tse nang le li-tubes tse hahiloeng ka hare (250μl-300μl).

2.2ml sampole ea molumo
Khoele ea Khoele ea Khalase, Polypropingne sampole ea boitsebiso ba polypropiplene.

3. Mohlala o moholo ho feta 2ml
4ml sampole ea 4ml sampole ea 4ml?

Ho utloisisa mefuta e fapaneng ea likopano tse sebelisoang ho li-chromatograph tsa metsi tse phahameng tsa metsi. Qobella sesebelisoa sena ho ithuta ho tseba ho khetha lipale tsa HPLC tse ntle ka ho fetisisa bakeng sa litlhoko tsa hau tse ikhethang:Encyclopedia of Hplc vaialis

Khetha ho latela mokhoa o koalang

Methapo ea methapo ea molomo hangata e hantle bakeng sa lc le lc \ Likopo tsa MS. Li fetoha mouoane le ho buleha hape. Ha ho bapisoa le cap ea Crimp, mokhoa ona oa ho koahela ha o kotsi matsohong 'me ha o hloka lisebelisoa tse ling. Li-cap tsa mohlala tsa sampole li fumaneha li-caps tse bohloko tse entsoeng bakeng sa bareki le li-caps tse tiileng tse etselitsoeng mohlala oa sampole.

Crimp:Lisebelisoa tse khethehileng lia hlokahala bakeng sa ho hlopha.Ba theha tiiso e sireletsehileng ea nako e atolositsoeng ea polokelo ha ba ne ba hatelloa hantle. Li-daial tsa sampole tsa crimp-caplus li loketse li-gc le gc \ Likopo tsa MS. Kaha li-cap tsa molao ha li khonehe, li fana ka tšireletseho e phahameng bakeng sa lijo, lipolanete le likopo tse ling moo boqapi ba sampole bo batlang. Haeba metsoako e sa tsebeng letho e sekasefele, litabeng tsa sampole tsa Crimp-plimp-Capple li khothalletsoa.

Li-snap tse holimo:Liphetoho tsa tiiso tsa bayonet ha li ntle joaloka mekhoa e meng e tiisang. Mohlala oa Bayonet Cappole ea Bayonet

Sebelisa setlotlolo sa peloana ea ho tiisa.Ho hanyetsa ha eona mokhofu le ho tiisa hore hoa khotsofatsa. Ha ho hlokahale hore ho be bonolo ebile ho bonolo ho e sebelisa, empa e hloka hore bansopomplers ba mmino ba sebetse.

Ho khetha call e loketseng bakeng sa bana ba li-chromagraph ba hmarmagraph ba bohlokoa bakeng sa ho netefatsa botšepehi ba mohlala mme ba fumana liphetho tse nepahetseng. Mona ke tataiso ho u thusa ho khetha letsopa le nepahetseng bakeng sa likhama tsa li-chromagraphy tsa hau tsa chromagraphy: Joang ho khetha cap ea hau e nepahetseng bakeng sa likhama tsa hau tsa chromagraphy?