Hobaneng HPLC e letsa tlhahlobo ea tikoloho ea tikoloho

Mokhoa o phahameng oa ts'ebetso oa chromatography (HPTC) ke mokhoa oa bohlokoa oa tlhahlobo o sebelisoang haholo ka tlhahlobong ea tikoloho ho bona, 'me mobu le moea. Joalokaha matšoenyeho mabapi le ho felisoa ha tikoloho o bakoang ke mesebetsi ea indasteri, ho hlophisoa ha litokomane, Litloaelo tsa temo e se li fetohile tsa tokelo ea ho patsa polokeho ea tikoloho le ho latela melao-motheo ea taolo.

HPTC ke mokhoa o rarahaneng o arohanyang likarolo ka motsoako o thehiloeng ho litšebelisano tsa bona le mekhahlelo ea seteishene le mobile. HPTC e fihlela qeto e phahameng le kutloelo-bohloko ka ho sebelisa khatello ea ho qobella mohlala oa mokelikeli ntlheng ea boemo bo phahameng. Karolo ena e ka bona hore na ba silafatsa lihlopha tse sa bonoeng ka mekhoa e fokolang e sa tsotelleng.

U batla ho tseba haholoanyane ka ho itokisa ha samc, ka kopo sheba sengoloa sena:HPTC sampole ea boitokiso ba litokisetso tse ntlehali

Melao e ka sehloohong ea HPLC ho tlhahlobo ea tikoloho

1. Teko ea boleng ba metsi

E 'ngoe ea tšebeliso ea sehlooho sa HPLC ho tlhahlobo ea tikoloho ke tlhahlobo ea boleng ba metsi. Silantants e kang litlolo tse boima tse kang merusu e mengata, meribeng, meriana e sibollang ba lik'hemisoa e ka baka likotsi tse kholo bophelong ba motho. HPTC e u lumella ho:

Tlhahlobo e porafatsang: HPLC e ka khetholla le ho khetholla lintho tse kotsi ka methapo e tlase haholo sampole ea metsi. Mohlala, posttic e kae e tloaelehileng e fumaneha ho Womoff ea temo e ka fumanoa.

Ho boloka chelete: Li-agency tsa taolo li hloka ho lekola mmele kamehla ea 'mele ea metsi ho netefatsa hore melao ea polokeho. HPTC e fana ka datha e tšepahalang e thusang ba boholong ho nka mehato e hlokahalang ho sireletsa bophelo bo botle ba sechaba.

2. Tlhahlobo ea Mobu oa Mohale

Mobu ke matrix e 'ngoe ea bohlokoa bakeng sa ho lekola tikoloho hobane e ka bokella mefuta e mengata ea lits'oants'o tsa tikoloho e hlahisitsoeng ke litloaelo tsa temo, mesebetsi ea ho lahla lintho tsa indasteri le ho lahla litšila. HPLC e bapala karolo ea bohlokoa ho tse latelang:

Tsebo ea li-pollnants: HPLC e thusa ho lekola boholo ba tšilafalo ka ho sekaseka lintho tse kang tsa polycycarbons (li-prulychlonis

Lits'ebeletso tsa ho khutlisoa: Ha mananeo a Bionerienri a reretsoe ho hloekisa libaka tse silafetseng ka ho lekola pherekano ea nako ka mor'a nako.

3. Ho beha leihlo la boleng ba moea

HPTC e boetse e sebelisoa ho sekaseka tšibollo ea moea le ho lekola boleng ba moea. Likopo li kenyelletsa:

Teko e sa sebetseng ea Volator (Voc) ea Voc) HPTC e ka arohana le ho khetholla metsoako ena sampole ea moea.

Tlhahlobo ea nako: Mokhoa ona o thusa ho supa bophelo bo kotsi bo ka amang bophelo ba ho hema le ho kenya letsoho tšilafalo ea moea.

4. Tlhahlobo ea litšila

Ho tsebahala ha litšila ho bohlokoa bakeng sa litloaelo tse nepahetseng tsa ho lahla le ho sebelisa mekhoa e meng. HPTC e ka u thusa:

Sekaseka melapo ea litšila: HPLC e thusa ho ngola mokhoa o nepahetseng oa ho fokotsa mokhoa o nepahetseng ka ho supa likarolo tse kotsi.

Etsa bonnete ba hore ho latela melao e sireletsehileng: Ho latela melao ea maemo ho hloka hore litšila li hlahlojoe ka botlalo ho fokotsa likotsi tsa tikoloho tse sa lokang.

Batla ho tseba haholoanyane ka lits'ebetso tsa li-varomatography tsa chromatography, ka kopo sheba sengoloa sena: Likopo tse 15 tsa likhama tsa chromatography masimong a fapaneng

Melemo ea ho sebelisa HPLC bakeng sa tlhahlobo ea tikoloho

Ho na le menyetla e mengata ea bohlokoa ea ho sebelisa HPPC ho tlhahlobo ea tikoloho.

1. Sensitivity e phahameng le ho nepahala

HPLC e ka bona maemo a sing a bona hore na o e kopanya ka thata bakeng sa ho hlokomela liporollo tse ka bang le litlamorao tse kholo tsa bophelo. Boikutlo bona bo phahameng bo lumella ho lemoha ha likotsi tse ka bang pelehi pele li kena mathateng a maholo.

2. Ho fapanyetsana

HPTC e fetohile sesebelisoa se fapaneng sa bo-ramahlale ba tikoloho hobane le sona se ka hlahloba se fapaneng le lihlahlobo tsa maphete a fapaneng (metsi, moea, moea). Mefuta e fapaneng ea li-lemono (UV - Plane, Feliorescence, boima ba rona ba lipono, jj) li ka sebelisoa ho latela litlhoko tse ikhethileng tsa tlhahlobo ea tlhahlobo.

3. Ho ba bokhopo

Ho ba le mabifi ha litsamaiso tsa HPLC ho li nolofalletsa ho sebetsana le matrice tse rarahaneng tse fumanehang hangata ho disampole tsa tikoloho. Mekhoa e nepahetseng ea mohlala ea boitokisetso ba ho itokisetsa ho khesoa e tiileng (e ka thusa ho itlhakisetsa maemo ho fokotsa litšitiso tse ka amang litholoana.

U batla ho tseba haholoanyane ka li-valiatograph tsa chromatograph tsa chromatograph bakeng sa tlhahlobo ea metsi, ka kopo sheba sengoloa sena: Malinyane a chromotography bakeng sa tlhahlobo ea boleng ba metsi

Sekhahla sa Ts'ebetso ea Choromagraphy ea metsi Likopo tsa eona li kenella ka thoko ho tlhahlobo ea boleng ba metsi, tlhahlobo ea bophelo ba mobu, e leng pono ea boleng ba mobu le eona e tlatsetsang kutloisiso ea rona ea mathata a bophelo. Joalokaha ho tsebisa lefatšeng ka tšitiso ea tšilafalo ea tšilafalo ea thibelo ea libaka tse hloekileng, karolo ea hipls e tla tsoela pele ho u tšehetsa bophelo ba sechaba le ho boloka linako tse hlakileng bakeng sa meloko e tlang.