Mino Vial Recert: Ho ntlafatsa ts'ebetso le katleho ho tlhahlobo ea HPLC

Kenya li-hiplc tse sa sebetseng hantle tsa HPLC Bahlahlobisisi ba ka sebelisakenya litebeleHo fihlela taolo e kholo ea mohlala, fokotsa kotsi ea ho tšoaea liphoso, e netefatsa ts'ebetso ea ho arohana, le ho ntlafatsa chromagraphinc ea karohano.
Mofuta o fapaneng oa ho kenya

Ho tsamaisa libaka tse nang le li-appsopler ho kenya li-vaialts tsa boikhathollo

Khokahano ea lits'ebetso tse ikemetseng tse kang AutoSosOlemplers, li feto-fetohe katleho ea tlhahlobo ea HPLC.Autosampler e kenya li-vaels, E etselitsoe ho amohela likarolo tsa bohlokoa tsa likokoanyana, li bapale karolo ea bohlokoa ea ho mela le ho ntlafatsa tlhahiso ea laboratoring. Liphaephe tsena li enjene ho kopanya ka mokhoa o seng monate le li-actisemplers, li thusa le enjene e sa fetoheng, le ho fokotsa phoso ea motho. Motsoako oa likhama tsa AutoSompler o kenya litlolo le litlolo tsa Mielise ho nolofatsa ts'ebetso ea tlhahlobo, ho sebetsana le lintlha tse kholo tsa mohlala ebe u fihlela tlhahiso e phahameng.
Ho hlahloba mefuta e sa sebetseng ea GC ho isa li-vaialis

Le ha HPLC ke mokhoa oa bohlokoa o sebetsang haholoanyane, khase ea khase ea khase (GC) o boetse o na le bohlokoa ba eona ho feta masimong a fapaneng.GC e kenye li-vaelsE entsoe ka ho khetheha bakeng sa likopo tsa khase tsa khase li fana ka phetisetso e khethehileng le ts'ebetso; Ha o kopantsoe le likhama tse loketseng tsa ho etsa qeto ea ho ntlafatsa, ho fokotsa likhakanyo tse fosahetseng tsa ho ntlafatsa mohlala o ntlafalitsoeng haholoanyane le o tšepahalang.
Microst ea Microst ea Micross: Ho thusa ho nepahala ho fokola ho feta bukeng e fokolang ea mehlala

Li-vialst tsa Microske lisebelisoa tsa bohlokoa bakeng sa ba mofuputsi ba sebetsang ka mantsoe a lekanyelitsoeng ea mohlala. Bophahamo bo bobe ba likhama tse nyane, hammoho le tlhahlobo ea metsotso e boima, e lumella tlhokahalo ea lintlha tse ngata tsa sampole. Morero o nepahetseng le o tiisang ka tatellano ea lik'hilomithara tse peli tsa likokoana-hloko ho thibela tahlehelo ea sampole, mouoane le litšila, boloka botsitso molemong oa tlhahlobo eohle. Ebang ke lijeong tsa meriana, tlhahlobong e meng ea tikoloho, lits'ollo tse ling tsa mahlale, li-microsty tse tsoang ho hlahisa bafuputsi ho fumana litholoana tse nepahetseng le tse nang le bothata ba ho ithuta.

Melemo ea 5 ea li-Iplc Insert: U ke ke ea khona ho ba teng

HPTC Insterts, ho kopanyelletsa le likopo tsa Mino Venive, fana ka melemo e fapaneng e lumellanang le tšimo ea k'hemistri ea sekaseka pele. A re hlahlobeng melemo e meng ea bohlokoa ea ho sebelisa lits'oants'o tsena ho tlhahlobo ea hplc:

1. Ho nepahala ha ho nepahala le ho nepahala:

Moralo o nepahetseng le boholo ba hiplc Insert, hammoho le likotlo tse kenyang likotlo, li netefatsa litholoana tse nepahetseng le tse khothatsang. Sebopeho se loketseng sa lisebelisoa tsa maphetetso tsa Mix Mix Sena se thibela lintho tse sa nepahalang tse bakoang ke sampole hape 'me li netefatsa tlhahlobo ea palo ea palo e etsoa ka mokhoa o hlakileng. Ts'ebeliso ea lits'oants'o tsa HPPC e ntlafatsa kutloisiso ea ho hlahlojoa, e nolofalletsa ho hlahlojoa ha maikutlo a tlase ka nepo.

2.

E 'ngoe ea liphephetso tse sa tsotelleng ho lihlahlobo tsa HPSC li boloka botšepehi ba mohlala ho fihlela se etsahale.HPTC InstertsBapala karolo ea bohlokoa ho boloka bo-'nete ka ho fokotsa lebitso pakeng tsa mohlala le bokaholimo ba eona. Sena se thibela ho sekaseka sebui, bohobe le ho silafatsa ho thibela lintho tse tšepahalang le tse sa lumellaneng le ho sa feleng. Lisebelisoa tse kahare tse sebelisoang ka har'a litlhomi tsa hipcst, tse kang khalase kapa li-polymers tse khethehileng, li fana ka tikoloho e loketseng bakeng sa pokello ea mohlala.

3. Kamohelo le AutoSoSoSoplers:

Liitic tsa bohlokoa li fetohile lisebelisoa tsa bohlokoa matsatsing a sejoale-joale, ho nolofalloa ke tlhahlobo ea lintho tse ngata. HPTC INST Kamohelo ea bona e tsamaisang chelete e sebetsang, e fokotsa litšenyehelo tse tsamaisang puo, 'me e eketsa mohato ka kakaretso; Bahlahlobi ba ka ba ho litloloko ho kenella setulong se fapaneng sa HPSc bakeng sa ho feto-fetoha le maemo le boiketlo ba ts'ebeliso.

4. Litlapele tse fokolang tsa ho ts'oaroa:

Tsamaea ka likepe, kapa sampole e tsoang liente tse fetileng, li ka ama haholo litlamorao le ho ts'epahala ha liphetho tsa liplc. Micro Vial Comet e bapala karolo ea bohlokoa ho fokotsa litlamorao tsa likepe ka ho fana ka thipa lipakeng tsa disampole. Bokhoni bo qotsitsoeng ka nepo tsa li-canpc tsa HPTC bo qobang ka ho thibela litšila le ho sekaseka litholoana tse nepahetseng leha o sebetsa le lihlahlobo tse rarahaneng kapa ha li ntse li sebetsa le lihlahlobo tse rarahaneng.

5. Ho fapanyetsana le ho se sebetse:

HPTC e tla ka boholo bo fapaneng, meralo le lisebelisoa tsa ho ikarabella ka litlhoko tse fapaneng tsa bahlahlami. Ho latela molumo oa mohlala, litlhoko tsa ho lumellana le sepheo sa ho li hlahloba, bafuputsi ba ka khetha ho tsoa libakeng tse ngata tsa Mino Vial. Lisebelisoa tse ling li ka fana ka meraki e khethehileng, joalo ka libopeho tsa litšupiso tsa ho kopanya kapa ho kopanya metsoako e ntlafalitsoeng bakeng sa ho fifala. Phapang ena e lumella bahlabali ba bona ho tsamaisa mekhoa ea bona ea HPPC ho lisebelisoa tse ikhethang, ts'ebetso e phethahetseng le ho fumana sephetho se nepahetseng ka ho se khone.

Ho khetha Likonteraka tse nepahetseng tsa HPLC
Ho khetha li-Impts tse loketseng tsa HPLC ka lebaka la tlhahlobo e itseng ho bohlokoa ho netefatsa ts'ebetso e nepahetseng le liphetho tse tšepahalang. Lintlha tse ling ke tsena tseo u lokelang ho nahana ka tsona ha u khetha li-hipls tsa hipc.

1. Kamohelo ea thepa:

Thepa eaHPTC Kopae lokela ho lumellana le mohlala oa mohlala oa matlix le sistimi. Lisebelisoa tse tloaelehileng li kenyelletsa khalase, e fanang ka mokhoa o hlollang ebile o fana ka Polymers e khethehileng, e fanang ka khang e khethehileng ea lik'hemik'hale. Nahana ka mofuta oa sampole le maemo a tlhaiso-leseling a ho khetha boitsebiso bo loketseng haholo.

2. Kenya moralo le boholo:

Ela hloko boqapi le boholo ba kenyelletso ea HPLC, ho kenyelletsa bolelele, bophara le sebopeho. Etsa bonnete ba hore ho kenngoa ha ho kenella ka tsela e sa tsotelleng 'me e lumellana le sistimi ea Autosampler. Boholo bo nepahetseng le ho hokahanya ha li bohlokoa bakeng sa ho tiisa maemo le sampole e nepahetseng.

3. Matla a molumo:

Nahana ka mohlala o lakatsehang oa ho bala mohlala oa ho bala ha hau bakeng sa tlhahlobo ea hau. Li-HPTC li fumaneha ka mathomo a fapaneng, hangata li tloha ho 0,1 ho isa ho 1 ml. Khetha kenya ka mokhoa o nepahetseng oa ho ntlafatsa boholo ba ho amohela boholo ba sampole ntle le ho sekisetsa maikutlo kapa ho nepahala.

4. Litšobotsi tse ikhethang:

Lisebelisoa tse ling tsa HPLC li fana ka likarolo tse ikhethang ho araba liphephetso tse khethehileng tsa tlhahlobo. Mohlala, lisebelisoa tsa koluoa ​​li khothalletsa ho kopanya hamonate, li bolela hore na homogeneity e sampole. Likotsi tse khutlisetsoang filting li fana ka mekhoa e tebileng ea ho tiisa bo tebileng, nyenyefatsa kotsi ea ho tsoa. Lekola litlhoko tsa hau tse ikhethang ebe u nahana ka hore na likarolo tse khethehileng tse fanoeng ke li-ints tse itseng tsa HPLC li tla tsoela pele ho hlahlojoa ha hau.

5. Kamano ea Mofani:

Ho khetha morekisi ea tšepahalang bakeng sa lits'oants'o tsa HPLC ke senotlolo sa ho boloka boleng ba sehlahisoa le botsitso. Batla barekisi ba nang le boiphihlelo ba ho ba le boiphihlelo ba ho fana ka lits'oants'o tse tšepahalang; Nahana ka lintlha tse kang litekanyetso tsa tlhahiso, li-netefatso tsa lihlahisoa le litlhahlobo tsa bareki ha u etsa qeto.

Likopo tsa lits'oants'o tsa hipc

HPTC InstertsFumana lisebelisoa ho feta lipapatso tsa saense le indasteri. Mona ke libaka tse tsebisang libaka tseo HPLC li rekisoang hangata li sebetsang hantle:

Ho hlahlobisisisisisisisisong:

Ho Laebo ea Memarong le Lipatlisisong tsa Bomati, HPLC ke sesebelisoa sa bohlokoa bakeng sa tlhahlobo ea lithethefatsi. Li-HPTC li ntse li thusa ka mokhoa o nepahetseng le tse nepahetseng tsa metsoako e nepahetseng ea meriana (apais) le litlolo tse pakang, ho netefatsa hore o latela maemo a phahameng a merafong. Ts'ebeliso ea lits'oants'o tsa maiketsetso tsa Mix e fokotsa litlhokahalo tsa mohlala, e etsa hore li tšoanele ho tšohla mehlala ea bohlokoa kapa e fokolang.

Tlhahlobo ea tikoloho:

Ho lekola tikoloho ha tikoloho ea tikoloho, joalo ka litlhare tse bolaeang likokoanyana, li hahlaila le lithipuoa ka metsing, mobu le disampole tsa moea. Li-hpc Insect li fana ka tlhahlobo ea li-feshene tse rarahaneng ka ho ntlafatsa sampole ho sebetsa, ho fokotsa litšitiso le ho ntlafatsa kutloelo-bohloko. Ho lumellana haHPTC Instertse nang le litsamaiso tse fapaneng tsa litsamaiso le mekhoa ea ho lemoha e etsa hore li fetohe bakeng sa tlhahlobo ea tikoloho.

Lijo le Teko ea Thript:

Tlhahlobo ea li-hplc tsa lijo le lisalp tsa Tloaele e sebelisoa haholo ho netefatsa polokeho ea sehlahisoa, boleng le ho latela melao e meraro. HPLC e bapalang karolo ea bohlokoa ea ho khetholla li-sistemen, masala a mokokotlong le a eketsang

Tlhahlobo ea Clinical le ea Qalelang:

Ka li-clinical le tsa mosali-moholo, tsa HPLC li sebetsa ho sekaseka li-biolonya tse fapaneng, lithethefatsi le lintho tse chefo tsa bioloji. Li-deferte tsa Michae li fana ka mohlala oa mohlala o nepahetseng le o tšepahalang, e lumellang palo e nepahetseng ea lihlahlobi tse rarahaneng. Ho tsamaisana le bona ka AutoSoslemplers bo ntlafatsa tlhahiso le katleho ea hotla le tlhahlobo ea bonono ea bongaka le tlhahlobo ea maikutlo.

Sephetho

Micro Via VialiansE fetotse tlhahlobo ea HPPSC ka ho ntlafatsa pono, ho nepahala le katleho. Matla a bona a ho fokotsa ho tahleha sampole ha a ntse a boloka mekhoa ea ho boloka libuka le lits'ebetso tse bolaeang tse bolaeang. Tšebeliso ea bona e tiisa litholoana tse tšepahalang ntle le hore na li sebelisetsoa ho lipatlisiso tsa Majeremane, kapa tlhahlobo ea bongaka e sa feleng.

Seo u lokelang ho ela hloko

Ho se hlokomele ka bokhoni ba ho ntlafatsa le ho fokotsa mokhoa o matla oa ho nepahala liqeto tse ling tse tšepahalang tsa mahlale.

Ikopanye le nna hona joale!