Hobaneng HPLC e sebelisetsoang tlhahlobo ea lithethefatsi?

Seaparo se phahameng sa chromatography (HPLC) e fetohile indastering ea tlhahlobo ea phapanyetsano mme e sebelisetsoa ho hlahlojoa ka lithethefatsi ka lebaka la ts'ebetso ea eona, le kutloisiso. Sengoliloeng sena se lekola hore na ke hobane'ng ha HPLC ke mokhoa oo o o ratang bakeng sa tlhahlobo ea eona ea lithethefatsi, ho qaqisa melao ea eona ea ts'ebetso ea ho hlahlobisisa lithethefatsi, menyetla le eona e sebetsa ho netefatsa polokeho ea lithethefatsi le katleho.

HPTC ke mofuta oa li-chromatograph tsa chromatography tse metsi tse sebelisang khatello e phahameng ea ho sotla ka lehare le tletse ka thepa e phahameng. Mokhoa o arola likarolo tsa motsoako o thehiloeng ho litšebelisano tsa bona le mekhahlelo ea seteishene le mobile. Likarolo li ka fumanoa ha li se li nyoloha le ho tloha khorong, li lumella tlhahlobo ea tšoaneleho le ea palo.

U batla ho tseba haholoanyane ka ho itokisa ha samc, ka kopo sheba sengoloa sena: HPTC sampole ea boitokiso ba litokisetso tse ntlehali

Melemo ea Bohlokoa ea HPLC ka tlhahlobo ea lithethefatsi

Boikutlo bo phahameng le khetho: HPTC e khona ho bona lintho tse tlase tse tlase, hangata ho fihlela boemo ba Nanogram kapa ba picogram. Kutloisiso ena e bohlokoa ha ho hlahlojoa lithethefatsi hobane ho eketsa litšila ho ka ba le phello e bohlokoa polokelong le ka katleho. Bokhoni ba ho arohana le metsoako e lekanang le meaho e tšoanang e ntlafatsa thuso ea eona ea ho sekaseka motsoako o rarahaneng.

Ho sebetsa hantle le lebelo: HPLC e na le nako e khuts'oane ea tlhahlobo e khuts'oane ho feta chromatography ea setso. Ho sebelisa pompo ea khatello ea khatello e phahameng ho lumella litheko tse potlakileng tsa phallo, e bakoa ka bokhutšoanyane ka mor'a nakoana ntle le boitelo tharollo. Bohato bona bo molemo haholo maemong a phahameng moo palo e mengata ea mesaletsa e hlokang ho hlahlojoa ka potlako.

Ho sitisoa ke li-limolek'hule tse fapaneng, ho kenyelletsa limolek'hule tse nyane tsa manyolo, liprotheine tse pepenoang, liprotheine le liprotheine tse ling. Ho fapanyetsana ha eona ho atoloha ho mofuta o fapaneng oa sampole, joalo ka lisebelisoa tse tala, menyako, metlaetetso le lihlahisoa tse felisitsoeng. Likomisisi tse fapaneng tsa Masinyane le Ho Fumana Tsebo li ka sebelisoa ho tsamaisa tlhahlobo ea litlhoko tse ikhethang.

Ho tsitsa le ho ikarola: Li-System tsa HLC li etselitsoe ho tsepamisa mohopolo, ho li boloka ho sebetsa khafetsa nako e telele. Sena se tšoantšetso hona ho tšoaea maemong a taolo ea boleng, moo melao ea mela e hlokang liphetho tse tšepahalang li hloka litholoana tse tšepahalang tse fetang likepe tse ngata.

Ho latela melao ea tsamaiso: Mekhatlo ea tsamaiso e kang FDA e hloka tlhahlobo e matla ea lihlahisoa tsa meriana ho netefatsa polokeho ea bona le katleho ea bona. HPTC ke mokhoa o amohelehang oa taolo ea boleng le tiiso ea ho fana ka litlhahiso. Bokhoni ba eona ba ho fana ka mohlala ka lintlha le ho netefatsa boitsebahatso ba metsoako ea meriana e sebetsang (APIS) e etsa bohlokoa ho etsa hore ho be le litekanyetso tsa taolo ea taolo.

Likopo tsa HPLC ho litlhahlobo tsa pharmaceutical

Taolo ea Boleng (QC): Lits'ebetsong tsa phaello, HPLC e sebelisoa ho lits'ebetso tsa taolo ea boleng ho netefatsa hore na u na le boiphihlelo, Matlafatso le Litšusumetso le Tse Hlōkang Ho Setlhane. E thusa ho lemoha litšila tse ka sitisang polokeho kapa katleho ea sehlahisoa. Mohlala, tlhahlobo ea botsitso e sebelisang HPPC e ka senola lihlahisoa tsa litlamorao tse ka hlahang ka mor'a nako tlas'a maemo a fapaneng a polokelo.

Ntlafatso ea lithethefatsi: Nakong ea kholo ea lithethefatsi, HPLC e bapala karolo ea bohlokoa ho e-ba le metsoako e mecha ea metsoako e mecha. Ho thusa bafuputsi ho utloisisa thepa ea lik'hemik'hale e kang ea sello le botsitso, ha e ntse e fana ka leseli litseleng tsa pharmacobineetic ka phaello.

Pharmacokickis: HPTC e sebelisoa haholo lithutong tsa pharmaconeetic ho sekaseka hore na lipilisi tsa Pharmackinetic li abeloe hore na lingaka li kenelletse joang, li ajoa, 'me li tlositsoe' meleng. Ka ho lekanngoe meroho ea lithethefatsi ka har'a maro a likokoana-hloko, tse kang mali kapa moroetsana, bafuputsi ba ka lekola katleho ea kalafo le polokeho.

Lits'ebetso tsa Bingalytical: Lithutong tsa bonyenyane, HPLC e thusa ho lekana le likotsi tsa lithethefatsi ka li-bilogies. Kopo ena e bohlokoa bakeng sa ho khetholla libakeng tse loketseng tsa ho senya le ho lekola likarabo tsa mamello nakong ea liteko tsa tlhaho.

Tlhahlobo: Ho tsebahatsa le ho akaretsa litlolo ho bohlokoa ho netefatsa boleng ba lithethefatsi. HPLC e fana ka tlhahlobo e qaqileng ea ho thusa bahlahisi ho utloisisa likotsi tse ka bang teng tse amanang le litšila kapa lihlahisoa tse litšila.

Karolo ea HPLC ho netefatsa polokeho ea lithethefatsi

Indasteri ea meriana e tsamaisoa ka thata mekhatlo e etselitsoeng ho sireletsa bophelo bo botle ba sechaba. HPTC e kenya letsoho ka sepheo sa bohlokoa ka:

Ho netefatsa boitsebahatso ba lithethefatsi: Ho netefatsa motsoako o nepahetseng o sebetsang ho teng ho tsepamisong e boletsoeng.

Ho lemoha li-sinminant: ho tsebahatsa litšila tse mpe tse ka etsang hore li be kotsi.

Ho boloka botsitso ba ho beha leihlo: Ho lekola sethunya sa lithethefatsi ka nako e fapaneng ho tseba bophelo bo loketseng ba sehatsetsing.

Ho tšehetsa litaba: Ho fana ka data ha ho hlokahala bakeng sa tlhahiso ea tsamaiso le tlhahlobo.

Batla ho tseba haholoanyane ka lits'ebetso tsa li-varomatography tsa chromatography, ka kopo sheba sengoloa sena:Likopo tse 15 tsa likhama tsa chromatography masimong a fapaneng

Liphephetso le Mehopolo

Ha Hplc e fana ka melemo e mengata, ts'ebeliso ea eona e boetse e na le mathata a mangata:

Ho rarahana ha nts'etsopele ea Mokhoa: Ho ba le mekhoa e sebetsang ea hipl ho hloka tsebo molemong oa chromatography hammoho le kutloisiso ea metsoako e itseng e hlahlojoang likotlonyana.

Lithabetso tsa litšenyehelo: Ho tsetela pele ka lisebelisoa tsa HPlc ho ka a makatsa, ho ekelletsa hore ho na le litšenyehelo tse tsoelang pele tse amanang le tlhokomelo, li-collum.

Mohlala oa mohlala o hloka: Litokisetso tse nepahetseng tsa sampole ho bohlokoa ho fumana litholoana tse nepahetseng; Ho hlophisoa ka mokhoa o sa lekanang ho ka fella ka tšilafalo kapa tahlehelo ea lihlahlobo.

Sekhahla se phahameng sa chromatography se fetohile sesebelisoa sa bohlokoa ho litlhahiso tsa meriana, katleho, ho sitisoa, le ho latela melao e laolang. Likopo tsa eona li fetoha ho tsoa ka matla boleng nakong ea ho etsa mosebetsi oa eona o boima ho tsoa ho lithethefatsi tse mpe tsa lithethefatsi le lipatlisiso tsa bionalyttical. Ha indasteri ea meriana e ntse e hola le ho hloka tlhahlobo e potlakileng, e sebetsang, e tiisa hore lithethefatsi li sireletsehile.

U batla ho tseba haholoanyane ka li-valiatograph tsa chromatograph tsa chromatograph bakeng sa tlhahlobo ea metsi, ka kopo sheba sengoloa sena:Malinyane a chromotography bakeng sa tlhahlobo ea boleng ba metsi