Ho khetha buffer e ntle ka ho fetisisa bakeng sa Mohala oa hau oa HPLC Mobile: Tataiso
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Ke buffer e ntle ka ho fetisisa bakeng sa karolo ea hau ea mobile ea HPLC?

Sep. 9th, 2024
Litags: Hplcanalysis Hplcvalstroalms Typesofhplcvials hipls-vials hplc-dele-vial-2ml li-khalase tsa likhalase 2ml-hplc-vialcs 4ml-hplc-vialcs
Ho khetha buffer e nepahetseng bakeng sa karolo ea hau ea mobile ea HPLC ea hau e bohlokoa ho fihlela karohano e nepahetseng le tlhahlobo ea hau sampole. Buffers Thusa ho boloka phallo e tsitsitseng, eo e leng ea bohlokoa bakeng sa ho laola ionization ea hau, ka hona e ama nako ea bona ea ho boloka nako le sebopeho sa Peak. Ka tlase ke kakaretso e hlalosang mokhoa oa ho khetha buffer e ntle ka ho fetisisa bakeng sa kopo ea hau ea HPSC.

Na ua tseba ka tokiso ea hplc e itokisa? Tobetsa Sengoliloeng sena ho Ithuta haholoanyane: "Ntho e ngoe le e ngoe eo u hlokang ho e tseba ka sampole ea HPPT ea ho itokisa"


Bohlokoa ba ho khetha ha Buffer

Buffer ke tharollo ea mang ha a fetohe kamora ho eketsa acid kapa setsi. Ho ipapisitsoe le sebopeho sa bona, ba buffers ba ka khethoa e le acidic kapa ea mantlha. Katleho ea buffer e khethoa ke pka ea eona, eo ka mokhoa o nepahetseng e lokelang ho ba nakong ea ± 1 p yuni ea ph ea mobile mobile ph e tsebahalang ea mobile ph e tsebahalang

Lintlha tsa Bohlokoa Tseo U Lokelang ho Nahanoa

1.PH: PH ea Mokhahlelo oa Mobile e lokela ho ba bonyane 2 PKA e hole le pka ea sekaseka. Sena se thusa ho hatella Ionization ea acidic kapa ea mantlha, e tlosa bolulo holim'a kholomo. Mohlala, haeba sekaseka ke acid e fokolang e nang le pka ea 4,5, u sebelisa mohato oa mobile ka pōpo e fetang 2

2.buffer Mofuta oa: Li-Buffers tse tloaelehileng tse sebelisitsoeng ho HPLC li kenyelletsa:

Li-Buffers tsa phosphate: E sebetsa ka har'a ph ea 2.0 ho isa ho 8.0 Li loketse lits'ebetso tse ntle haholo tsa LC-UV.


Li-Buffers tsa Acetate: Tsena li boetse li tsebahala bakeng sa LC-UV li ka sebelisoa ho PHSSPOTE hore e be li-buffers tsa phosphate.

Li-buffer tse ikhethileng: Bakeng sa lits'ebetso tsa LC-MS

3. Khafare ea 3.Buffer Contication ea ho qala bakeng sa li-bulfrs ho HPTC ke pakeng tsa 25-50 mm. Maatha a phahameng a ka ntlafatsa sebopeho sa Peak le katleho empa le tsona li ka lebisa ho pula ha o tsoatswa. Ho bohlokoa ho boloka buffer tsepamisa maikutlo ka tlase ho 25 mm ho qoba mathata.

4.Comical hloeke: Sebelisa li-bufroma tse phahameng haholo tsa chromagraphic-li-bufragraph tsa sehlopha ho thibela tšilafalo le ho netefatsa sephetho se tšepahalang. Litšireletso li ka lebisa tšebelisanong le phapang e sa lebelloang le maemo a ho thibela mesebetsi.

Liphoso tsa mocheso: Mocheso o ka susumetsa pH ea tharollo ea buffer. Ho bohlokoa ho lokisa le ho boloka barekisi ka mocheso o ts'oanang le ho boloka katleho ea bona.

Batla ho tseba likarabo tse 50 mabapi le li-valc vials, ka kopo sheba sengoloa sena: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

Malebela a Sebetsang Bakeng sa Boitokisetso ba Buffer

Mokhoa o hlophisitsoeng: Li-buffers li ka lokisoa ka ho kopanya likarolo tsa tsona tsa acidic le tsa mantlha kapa ka ho khothatsang tharollo ea mantlha le acid. Qoba ho fetola Buka kamora ho eketsa li-solvent tsa manyolo, kaha sena se ka lebisa liphellong tse sa lumellaneng.

Litharollo tse ncha: Lokisetsa tharollo e ncha ea buffer khafetsa ho fokotsa kholo ea likokoana-hloko le ho nyenyefatsa metsoako, e ka amang tšebetso ea li-chromatographic.

Ho Qoba Tsire: Babuelli ba itseng, ba kang Ketsahalo, ba ka sekisetsa likarolo tsa tšepe tsa system. Etsa bonnete ba hore sistimi e potlakileng ea sistimi ka mor'a ho sebelisa babuelli joalo ho thibela tšenyo.

Sephetho

Ho khetha buffer e nepahetseng bakeng sa Mobile Hplc Mobile ho fihlela sephetho se tšepahalang le se hlahisoang. Nahana ka pH, mofuta oa Buffer, ho tsepamisa mohopolo le bohloeki ha u khetha. Ka ho latela litataiso tsena, u ka ntlafatsa mokhoa oa hau oa HPPT ho arola le ho sekaseka mehlala ea hau.

Batla ho tseba theko ea li-valc vaialis, ka kopo sheba sengoloa sena: PLPC Valias theko: Lipotso tse 50 tse botsoang khafetsa
E fetileng:
Mokhoa oa ho Sebelisa Liaparo tsa Cod ka nepo bakeng sa tlhahlobo ea boleng ba metsi?
E 'ngoe:
Mokhoa oa ho Qoba ho Filter Phoso ea Filding e Filringe
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