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Lithako tsa chromatography bakeng sa tlhahlobo ea Deusternic To Lekano

Oct. 9th, 2023
Litags: Tlhahlobo ea lik'hemik'hale Litlhahlobo tsa lithethefatsi Taolo ea boleng Li-hplc-vials-vials-vials-vials 2ml-hplc-vial-with-cap hplc-dele-vial-2ml li-hipsamp-appsopler-vials hipls-vials Li-chromatography-Valis Vias-chromatography-2l
Maikutlo a fapaneng ke karolo ea bohlokoa ea mahlale a melimo ea maikutlo, a bapala karolo ea kelello lipatlisiso tsa molao tsa botlokotsebe le linyeoe tsa molao. Litsebi tsa 'mele li sebelisa mehlala ea biolonical e kang mali, moroto le oa lisampole tsa moroto ho bona lithethefatsi, joala kapa lintho tse ling tse chefo hona joale. Ho fihlela liphetho tse nepahetseng le tse tšepahalang tseo ba sebelisang li-chromatography tsa khase (gC Sehloohong sena re hlahloba bohlokoa ba bona ba likarolo tsa tlhahlobo ea maikutlo a sa tsebeng ho hlalosa lintho tse ngata.

Choromatography to defficle


Mokhoa oa chrotography ke mokhoa oa bohlokoa o sebelisitsoeng ho chefo ho khethoa le ho khetholla likarolo tse fapaneng tsa teng. Ho ipapisitsoe le melao-motheo e fapaneng ea karohano, e hlokang ho arola mehlala pakeng tsa mohato oa mobile le mobile; Metsoako ea mohlala e tla sebelisana ka tsela e fapaneng le mekhahlelo ena e lebisang karohano ho latela thepa ea lik'hemik'hale e leng sampole.

Mekhoa ea Chromatographic e Fora


Litsebi tsa che tsa 'nete tsa chefo li sebelisa mekhoa e fapaneng ea li-chromatography, ho latela tlhahlobo eo ba e hlahlobang:

Khase ea khase ea Khase: Khase e entsoeng ka khase e sebelisoa haholo bakeng sa tlhahlobo ea lintho tse sa utloahaleng tse kang joala joalo ka linohi. Ho sebelisa GC ka katleho, lisamompole li tlameha ho tepella pele ho tsebisoa kholomong ea boemo bo phahameng bakeng sa ho arohana ho latela khatello ea li-vapor kapa ho ikarola karolong ea hae ea mouoane; Li-vaels tse khethehileng tse entsoeng ka ho khetheha bakeng sa ts'ebetso ena li netefatsa ente e nepahetseng ka tahlehelo e tlase nakong ea ts'ebetso ena.

Liroko tsa chromatography tsa metsi Ho khanna tlhahlobo e nang le mokhoa ona, mohlala ona o tlameha ho qhibiliha ka har'a karolo ea mehala ea mobile pele e feta mothating oa ho hlahlojoa. Mefuta e fapa-fapaneng ea li-colums kapa li-vaiale li ka sebelisoa ho latela litlhoko tsa 'ho arohana le litlhoko tsa karohano.

Bakeng sa botebo ba ho sheba lits'ebeliso tse 15 tsa likhathatso tsa ciaromatography, hlahloba sengoloa sena se rutang:Likopo tse 15 tsa likhama tsa chromatography masimong a fapaneng

Lithako tsa ciaromatography li na le li-heroes


Lithako tsa Chromatograftke likarolo tsa bohlokoa tsa mekhoa ea chromatography, hangata e hlokomolohuoa ka lebaka la ho tsepamisa mohopolo mekhoa ea ho sekaseka. Leha ho le joalo, li-vial tsena li bapala karolo ea bohlokoa ho boloka tlhahlobo ea mohlala ea ho tšepahala le ho nepahala:

Lintho tse amanang le tumello:


Hangata, li-vies li hahuoe ka khalase e borolicate kapa lisebelisoa tsa polasetiki ea polasetiki. Khalase ea borolisicate e khethoa ka lebaka la khanyetso ea eona e phahameng ea lik'hemik'hale le ho ithaopela; Lisebelisoa tsohle li tlameha ho lumellana le mokhoa oa mohlala oa Matrix le Chrotographic in beed ho qoba ho opa ha litšila ka tharollo.

Size le molumo:


Boholo ba boholo le molumo o tlameha ho kopana le litlhaloso tsa sampole le litlhaloso tsa seletsa bakeng sa liphetho tse phethahetseng. Li-vaels tse nyane li sebelisoa hamolemo bakeng sa masela a lekanyelitsoeng ha li-vaels tse kholo li lokela ho sebelisoa ha ho sebetsana le mehlala ea bulkier. Qetellong, le ho koaheloa hoa phello le ho koaheloaloa ho boetse ho lokela ho hlahlojoa.

Ho tiisa le ho koaloa:


Mekhoa e nepahetseng ea ho tiisa le ho koaheloa ke ho tšoaea mouoane le tšilafalo, ka li-cap tsa scp kapa li-cap tsa snap.

Bohloeki le bohloeki:

Litoro tsa ciaromagraphy li lokela ho hloekisoa haholo ho tlosa masala kapa lithibelo, ha likhama tse ling tse hloekisitsoeng pele li netefalitsoe hore li kopane le maemo a bohloka. Mekhoa ea taolo ea boleng le netefatso e tlameha ho bonoa nakong ea ho hloekisa.

Taolo ea boleng le netefatso


Ho nepahala, ho nepahala le ho ts'epahala ho bohlokoa ka ho fetisisa ho Prography ea Mano a Mamani ea Meqathatso ea Li-capenes le li-protocol tsa boleng bo hlophisehileng le tse sebetsang. Lithuto tsa netefatso li hloka ho lekola litlolo tsa chromatography tse kang likotlo tse boletsoeng ho tsebahalang, ho nepahala le mekhoa ea kutlo; Tlhokomelo e tloaelehileng le ho lekanya lisebelisoa tsena - ho kenyeletsa litsamaiso tsa li-vial tse sebetsang tsa Via - hape li bohlokoa ho fihlela sephetho se sa fetoheng le se nepahetseng.

Sephetho


Lithako tsa Chromatograftke lisebelisoa tsa bohlokoa tsa ho ipolella ho Dexicology la Volicoloology, ho fana ka karohano e nepahetseng ea metsoako e ka har'a samoloji ea biologist. Ha u khetha likhama tsena bakeng sa ho sebelisa, ho nahanela ka hloko ka hloko ho latela sebopeho sa mohlala ka bobeli le mekhoa ea li-cirmatography. Mekhoa ea taolo ea boleng le netefatso e ntlafatsa litholoana tsa lipatlisiso tse shebahalang, ho fana ka tiiso ea ho phehella toka ho latela lintho tse chefo tse amang lintho tse nang le chefo tse amang lintho tse chefo. Tsoelo-pele ea mahlalehla e thusa ho tsoa ho ntlafatso ea li-ciaromagraphy Ba nka karolo ea bahale ba khutsitseng ba hlokomela toka e eteloa ke matichere a saense le ho nepahala.
Bakeng sa tlhaiso-leseling e akaretsang ea litšoantšo tsa HPLC, sebelisa ho sengoloa sena se rutang:Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang
E fetileng:
18mmm choromatographs: 3 Lintlha tsa Khetho tsa Khetho bakeng sa tlhahlobo e kholo
E 'ngoe:
Tsohle tseo o hlokang ho li tseba ka mefuta e fapaneng ea lisebelisoa tsa Septa bakeng sa li-valias tsa chromatography
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