Li-headpace li hloekisa le ho sebelisa hape: ho sebetsa, papiso ea theko e tlase ea li-vavers tsa discoed vs.

1. Selelekela

Li-shelspace tsa lihlooho li etsoa ka khalase e borolicate - li sebelisoa haholo ho gc le gc-m ms ho sekaseka metsoako e sa tsebeng Heatpace. Ba balse ka mor'a ho hloekisa ka nepo le ho mo fanyeha feela ho ka fokotsa litšenyehelo tse sa lokelang feela empa ba ts'ehetsa hape le ho tšehetsa melaele.

2. Mefuta ea Vialive le lits'ebetso tse loketseng

• Screw-cap vials (screw-top): Ho bonolo ho bula \ Haufi, e lumellana le li-autoosoplels tse ngata, 'me li loketse tšebeliso e phetoang ka tlhahlobo e phetoang hangata ka tlhahlobo ea VOC.

• Li-vaialis tsa Crimp-Cap (Aluminium Crimp Lid + Septa): Fana ka tšusumetso ea kelello, hangata e sebelisoa ke hore e se e le masoha hobane setlamo se baka Deformation. E ne e khetha khatello e phahameng, volatility, kapa e nang le tlhaiso-leseling e tiileng (e.g. Hindic, lijo, pharmautical).

3. Ho hloekisa mosebetsi le ho tlosoa ha masala

Ho Itokisetsa:

• Sampole e sa lefelloeng.

• Scrub botlolo ea botlolo ka brashi kapa mothetsi.

• Tlosa le ho hloekisa cap le septa ka thoko.
  
  

Litsamaiso tse tloaelehileng tsa ho hloekisa tsa batho (e fetotsoe ho tsoa ho Protocol ea Lab):

Mokhoa o (kakaretso ea masea a fapaneng)

1. Soak ka 95% ethanol

2. Ultrasonic e hloekile habeli

3. Hlatsoa habeli ka metsi a behiloeng

4. Oven-omileng ho ~ 110 ° C bakeng sa lihora tse 1-2

Mokhoa oa B (e thehiloeng metsing \ e tlase)

1. Rinse e nang le metsi a pompo khafetsa

2. Ultrasonic ka metsing a qholotsoeng (15min × 2)

3. Soak in athany ethanol hape e omisitsoeng

Mokhoa C (Metanol-a Matla)

1. Methanol soak + 20min ulttrasonic

2. Metsi altrasonic (20min)

3. O hlahe hantle

Mokhoa oa D (oxdiding e matla e hloekile bakeng sa litšila tse ngata)

1. Lense ea Acid ea Acid: Sulfuric Acid + Wastium Dictroromate sok → Rinse

2. Fello ea bongaka ea bohlanya ≥4H + 30min ultrasound

3. Metsi Ulrasound Arse → Omileng

Mokhoa o (oxidative + e theko e boima)

1. 24h soake ka potasiamo ea potasiamo

2. Metsi a Metsi a Metsi a Seketsoana sa Metsi (× 3)

3. Methanol Rinse → Air Omella

4. Kamehla nkela septa \ khalase ea khalase ha u batla relisa

4. Desorition pre-kalafo

Ho fokotsa masala a makatsang a ho velea:

• Ho futhumatsa mocheso ho oven (110-150 ° C) bakeng sa lihora tse 1-2.

• Boima ka boikhethelo bo nang le khase ea methapo kapa lipotoloho tsa vacuum.

• Holisa equilibration nakong ea gc factpace incubation ho thusa matšeliso.

Mehato ena e fokotsa "litlhoro tsa moea" le lerata la moraohali ho GC Bahlomphehi.

5. Netefatso ea boleng ba boleng

• TLHOKOMELISO EA LITLHAKISO: Sebelisa tlhahlobo ea toc kapa li-enstation tse se nang thuso tse nang le litlhoro tsa li-gc-hs mme li bapisa litlhoro tsa maemo a tlase ho ba tsoang masea a bacha ho netefatsa litlhohulong tse sa lebelloang.
  
  

• Mokhoa oa ho netefatsa mekhoa: Ts'ebetso (e pheteletseng), sekhahla sa ho hlaphoheloa (ka litekanyetso tse spiked), meeli ea ho lemoha - meeli ea ho lemoha - e bohlokoa ho netefatsa hore matsali a macha a a macha.

• RAND REVENES: Lekola palo e 'ngoe le e' ngoe ea Velic ea ho etsa lipotoloho; Etsa meeli ea meeli (e.GE 3-5 e sebelisoa). Boloka litlaleho tsa ho hloekisa, liente tsa nako le nako le tlhahlobo ea terdown.
  
  

6. Sebelisa li-phispan le likotsi

• Ka tloaelo, li-vaialis tsa Borosilicate li ka sebelisoa ka mokhoa o sireletsehilengHoo e ka bang makhetlo a 3-5Kamora ho netefatsa lits'ebetso tsa ho hloekisa le QC.
  
  

• Likotsi tsa Tšebeliso:

  • Ho tšoantšetsa - ho tšoaea liphoso

  • Septum deform kapa ho tlosoa hoa tsietsa tiiso

  • Tšenyo ea khalase ea khalase (likhetla, ho tloha botjha

  • Ho fapana ho hloekisoa pakeng tsa li-vials le methapo e tlisa tlhahiso e mpe
    
    
    

7. Kutteland

Li-Labs tse ngata, litšenyehelo tse patiloeng (basebetsi, QC, li ntse li tšehetsoa, ​​li mathela ka tsela ea ho sebelisa le ho sebelisa mokhoa oa maemo.

8. Litlhahiso le mekhoa e metle

• Khetha mokhoa oa ho hloekisa o thehiloeng ho samonation ea litšila; Sebelisa li-protocol tse matla tse oxidative ha feela ho hlokahala.

• Kamehla nka sebaka sa Septa; Beps vers \ Septa e lebisang ho lutla le deformation.

• Ho kenya letsoho litlolo tsa ho hlophisa libotlolo tse litšila tsa ho hloekisa, ho lelekisa palo ea tšebeliso le likhoerekhoere tse ngata.

• Nako le nako e netefaletsa le liente tsa gc le liente tse se nang letho; Lahla libotlo hang ha QC e hloleha kapa ka mor'a potoloho ea hau ea unel.

• Bakeng sa lithupa tse phahameng kapa tse ling

• Basebetsi ba koetlisa ho etsa bonnete ba ts'ebetso e tšoanang le e bolokehileng, ho kenyelletsa le ts'ebeliso ea PPE ha e sebetsana le acid le li-soeling.
  
  

Kakaretso

• Ho hloekisoa ka botlalo, ho hloekisang mohato o mengata ho kopantsoe le mokhabiso o futhumatsang oa hlooho ho ka etsa hore ho be le lits'oants'o tse ngata tse nang le likhama tse ngata ntle le ho inehela.

• Leha ho le joalo, mokhoa oa ho pheta-pheta o hlahisa ho rarahana: Mosebetsi, lisebelisoa le li-labs tsa QC li ka feta litsobotsi tse theko e fapaneng kapa tse nepahetseng.

• Ho kenya tšebetsong linnete tse hlakileng le lits'ebetso tsa netefatso li lumella mathoaso a moruo, a nang le boleng ba tikoloho le boleng ba tikoloho ka ho beha kotsi hantle.