Sep. 5th, 2023
Mekhoa ea karohano ea lik'hemik'hale e se e le karolo ea bohlokoa ea lits'ebetso tsa sejoale-joale sa mahlale, ho tsoa Promacerial ho ea tlhahlobong ea tikoloho. Ho beha chate ea chromatograph ea mantlha ho khetholla metsoako e mengata - mekhamation ho ea tikoloho. Karolo ea bohlokoa ea chromatography hangata e hlokoang ke basebelisi ke eona ea bohlokoa ho sammopole e fumanehang; Lits'ebetso tsa morao-rao tse entsoeng ka moralo oa morao-rao li na le katleho e kholo ea mekhoa ea karokose ea lik'hemik'hale ha e ntse e fana ka bafuputsi le likhoebo tse ntlafalitsoeng.
Karolo ea likhathatso tsa chromatography
Mokhoa oa chrotography ke mokhoa oa tlhahlobo o sebelisitsoeng ho arola motsoako o rarahaneng ho likarolo tsa tsona. E sebetsa ka ho sebelisa likhakanyo tsa mehlala le mekhahlelo ea seteishene le mobile - ka tloaelo - ho latela litsamaiso tsa chromatography tsa chromatography. Liphetho tse tšepahalang li itšetlehile ka lits'ebetso tsena hammoho le mehlala ea mohlala - ena ke moo li-vaias li kenang teng.
Litsi tsa moetlo li sebelelitse ka morero oa tsona lilemong, leha ho le joalo hangata e le mefokolo e thibelang karohano e hlakileng le e sebetsang ea lik'hemik'hale. Litaba tse ling tse tloaelehileng li ka kenyelletsa:
Ho silakanya ka li-viias:Litsomi tsa setso li ka silafatsa mehlala ka ho kopana le libaka kapa li-cap, li lebisa liphethong tsa bo-ramahlale 'me li ka fokolisang.
Mouoane le sampole tsa sampole:Li-vaels tse matla tse tiisitsoeng li ka lumella mouoane oa tulvent, ho fetolela mohlala oa khatello ea maikutlo le ho tšepahala ha tlhabollo.
Molumo o shoeleng:Mekhoa ea meeri ea setso ea setso e ka etsa molumo o matla oa ho shoa, o etsa hore ho be thata ho fumana lerotholi le leng le le leng la ho qetela la mohlala oa bohlokoa oa mohlala oa bohlokoa oa mohlala oa bohlokoa oa mohlala oa bohlokoa oa mohlala oa bohlokoa oa mohlala oa bohlokoa oa mohlala oa bohlokoa oa mohlala oa bohlokoa oa sampole ea bohlokoa.
Ho lumellana:Ha se li-vaels tsohle tse loketseng ts'ebeliso e 'ngoe le e' ngoe, mocheso le ho ikamahanya le mekhoa e fumanoang ho lemoha.
Boqapi ba liphatsa tsa chromatography
Tsoelo-pele ea morao-rao hoMoralo oa Veliographyba amohetse mathata ana ka ho fana ka tharollo ea karohano ea lik'hemik'hale tsa lik'hemik'hale tsa lik'hemik'hale.
Pholoso e Phahameng ea mohlala:Li-vaialies tsa sejoale-joale li hahiloe ho thepa e nang le thepa e tlase ea adsorption, fokotsa tšebeliso ea mohlala bakeng sa pokello e nepahetseng ea data e nepahetseng le e tšepahalang.
Mekhoa e Ntse e Ntlafatsang ea Mecha:Malinyane a sejoale-joale a nang le mechini e phahameng e tsitsitseng, a kang li-cap tsa ho nepahala, tse thibelang mouoane le sampole.
Molumo o Nyane o Nyane:Mekhoa e metseng e meholo e mengata e ka fokotsa libuka tse shoeleng, tsa thusa bafuputsi ho iphelisa ka mehlala ea bona.
Baetsi ba Vial ba fana ka mefuta e mengata haholo:Bahlahisi ba sejoale-joale ba fana ka khetho e nyarosang ea likopano tse ikhethileng, ho kenyelletsa mocheso o phahameng le tikoloho.
Melemo e 4 ea ntlafatso ho li-vaeromatograph tse ncha tsa ciaromatography
Melemo ea ho amohela litabeng tsena tse ncha tsa ciaromagraphy tse totobatsoang:
Boleng bo ntlafalitsoeng ba data:Pholoso e senyehileng ea mohlala mme e fokolitse molumo o shoeleng o ka ntlafatsa ho nepahala ha data le ho ikatisa, ho fana ka liphetho tse nepahetseng le tse phetoang.
Nako le Chelete ea Lichelete:Mekhoa e Ncha ea Bohlokoa e felisa tlhoko ea tlhahlobo e mengata ka lebaka la ho ba mouoane kapa tahlehelo, ho boloka nako le lisebelisoa.
Ho hlaphoheloa ha mohlala:Bafuputsi ba ka fumana mehlala e eketsehileng ea bona, ho etsa bonnete ba hore metsoako e molemo e ea lela.
Ho fapanyetsana: Ho ts'oaroa ha ViaKa maemo a mangata le mekhoa ea ho lemoha e eketsa maemo a lihlahlobo tsa li-chromatographic.
Ho hatela pele tsoelo-pele ea likhahla tsa ciaromatography
Tsoelo-pele e 'ngoe ea liketsahalo tsa Geomatography e entsoeng ka li-vaels tsa chromatography e le sebaka sa lisebelisoa tse ncha. Hangata li-viels tsa setso li entsoe ka khalase ka lebaka la botle ba lik'hemik'hale, le ho ba le boikarabello ba likopo tse fapaneng; Leha ho le joalo, li-vaelis tse khalase li na le mathata a itseng a ho thibela litšila hore li be sampole.
Lithibelo tse ncha tsa ciaromagraphy li etsoa hangata ka lisebelisoa tse tsoetseng pele tse kang li-Plasttics tse kang polypropingne kapa polyethylene, e fana ka melemo e mengata:
Chemical byhalerness:Li-pilytric li-vies li na le sebaka se fokolang bakeng sa litlhahlobo, monyetla oa ho fokotsa monyetla oa ts'episo kapa tšilafalo e ka 'nang ea hlaha ka litlolo tsa khalase.
Ho Boloka:Li-polymet lirile li hanana le ho lisa ho feta mefuta e meng ea khalase, e etsang hore li lokele tšimo libakeng tse thata.
Pepenene:Li-valymer tse ngata tsa polymer li na le lingoliloeng tse hlakileng, li nolofalletsa tlhahlobo e ntle ea mohlala le maemo a tlhaho.
Kotsi e fokotsoang ea mosebetsi oa Silanol:Libaka tsa khalase tse sebelisoang chromatography tse sebelisoang li-chromatography li ka bonts'a ts'ebetso ea Silanol le ho kena-kenana le mefuta ea mantlha; Liphaephe tsa polymmet ha li hlahise taba ena.
3 LITLHAKISO TSA BOPHELO
Lipolelo tsa ho koaloa ka bongata li boetse li nkile karolo ea bohlokoa ho feta tekanyetso ea lits'ebetso tsa karohano ea lik'hemik'hale tsa lik'hemik'hale:
Septa Lisebelisoa:Septa ke litiiso tse arohaneng li-sampole tse arohaneng libakeng tsa bona tsa likoli. Lisebelisoa tsa sejoale-joale tsa Seteli tsa bojoale-joale li etselitsoe ho fokotsa litšila le ho eketsa ts'ebetso ea ho tiisa; Mehlala e ka kenyelletsa silicones,buyl raber le PTFE (Polytetraflueroethylene).
Slit Septa:Ka ho tlosa ho phunya ha matsoho le likotsi tsa eona tse amanang le tšenyo le tšilafalo,pele ho septae phallela letheka le ho boloka nako le liphoso tse ka bang kotsi. Ntho ena e boloka nako le lisebelisoa tse bonoang ha li ntse li fokotsa mehloli ea phoso.
Ho sebetsana le mechaisiss:Mefuta ea sejoale-joale e fana ka mefuta e mecha ea mecha, joalo ka li-cap tsa screw tse nang le litiiso tse botsoang bo fallisoa. Meetso ena e netefatsa hore e tiisoa ebile e ka tšeptjoang ea Toropo e nyane - e felisang kotsi ea ho tiisa kapa ho tiisa cap.
Lintho tse entsoeng ka tloaelo le tse ikhethang (CRVIASS)
Bafuputsi ba lula ba kopana le mathata a ikhethang ha ba khanna lihlahlobo tsa li-chromatographic tsa li-chromatographic, ho fumana likhathatso tsa setso. Baetsi ba lihlahisoa hona joale ba fana ka likhetho tse ngata tsa ho etsa lintho ka mokhoa o ikhethileng ho tsoa ho lintho tse tloaelehileng tsa sebopeho ho lisebelisoa ho rarolla litlhoko tsena. Mehlala e meng ea matsala a ikhethileng e kenyelletsa:
Li-headSpace li-vialspace:Li-vaels tsena li tsamaisoa ka kotloloho bakeng sa li-chromatograph tsa khase (gC), e nang le molatsoana oa samppace sa Postling - e bohlokoa ka tlhahlobo ea maikutlo.
Micro-Insert:Bakeng sa mehlala e nang le molumo o fokolang,Micro-contsE ka thusa ho fokotsa molumo o mofu oa mofu 'me ho ntlafatsa ho hlaphoheloa ke mohlala. Li lokela ho beoa ka har'a masela a tloaelehileng e le ho fokotsa sebaka se shoeleng le ho ntlafatsa polelong ea mohlala.
U batla ho tseba haholoanyane ka li-intc tse ngata tsa HPLC, ka kopo sheba sengoloa sena:HPTC Vial Intrys: Elisa ho nepahala le ho ba le botshepehi
Likopano tse holimo tsa Crimp-Top: Li-vimp-li-vimp tse tobilengli sebelisoa hangata likopo tsa khase tsa khase 'me li hlahisa molala o pholileng ka tiiso ea kotara e ka sitisoang bakeng sa ho koaloa ha sampole ea mahala. Moralo ona o tiisa ho boloka polokelo e sireletsehileng.
3 Sekaseka Ts'ireletso ea tikoloho
Lits'ebetso tsa matsoalloa a ciaromagraphy li ea ntlafatso ea tšebetso; Ho tsitsa tikoloho hoa tikoloho ho ntse ho eketseha ka mehla ha ba li pota-pota-poti ea saense le indary ka mokhoa o tšoanang. Lisebelisoa tse ncha tse entsoeng ka polyers tse tsoetseng pele hangata li na le tikoloho e sa tsoakoang hangata ha ho bapisoa le li-vaels tsa khalase li kenyelletsa:
Phokotso ea maoto a carbon:Tlhahiso ea likhama tsa khalase e hloka ts'ebeliso ea matla e kholo; Hangata, li-vies tsa polymetri li sa khone ho ba boima ba fokolang mme li hloka matla a fokolang bakeng sa tlhahiso le lipalangoang; Boima bona bo tlase le matla li fella ka ho fokotsoa ha leoto le fokolitsoeng lijong tse fokotsitsoeng.
TLHOKOMELISO:Liphaephe tsa Polymer li ka sebelisoa hape, li fana ka mofuta o mong oa ho lahla. Ka mekhoa e thusang e le hantle, tšusumetso ea bona ea tikoloho e ka fokotsoa hape.
Litšenyehelo tse tlase tsa lipalangoang:Li-vaialis tse bobebe li hloka matla a fokolang bakeng sa lipalangoang, ho khaola ho sebelisa mafura le ho tsoa nakong ea thomello.
Nako e telele ea POLYMER e li lumella ho li-vaerate tse ntle tsa khalase, tse fokotsang tlhahiso ea litšila.
Litloaelo tsena tse ncha tsa chankamagraph li fana ka ts'ebetso e phahameng ea tikoloho, e etsa khetho e lakatsehang bakeng sa li-laboratories le indasteri li sheba ho fokotsa maoto a bona.
4 Mokhoa oa Bokamoso oa Maganotographic Movie ea Via
Joaloka tšimo ea li-chromatography, kahoo hape e tla etsa hore ho be le litlhoko tse phethahetseng tsa ho iphelisa ka mokhoa o sa feleng oa bafuputsi le indasteri ka mokhoa o tšoanang. Mekhoa e meng e neng e lebelletse hore mekhoa e menyenyetsi ea nako e telele ea li-curiatography e kenyelletsa:
E le meralo ea laboratori e ba meralo e atileng, e ka ba e kenyelletsang likarolo ho tsamaisa liroboto tsa liroboto le ho sebetsa.
E bohlale:Li-vaes tse hlometseng li na le bokhoni ba sensor kapa tsa data tsa data li ka fana ka leseli la nako ea 'nete mabapi le maemo a sampole joalo ka mocheso le khatello ea data.
Tsepamisa maikutlo ho ho tsitsisa:Baetsi ba thepa ba ka beha thepa le lits'ebetso tsa botsoalle e le ho fihlela lipakane tsa bona tse matlafatsang.
Technology ea Bohlokoa Technoloji ea Bohlokoa:Tsoelo-pele mabapi le theknoloji e matla e ka fella ka ho koaloa le ho feta, ho koala se fokotsang se fokolang le ho feta se tlohang ho fokotsa kotsi ea tšoaetso kapa tahlehelo.
Nanotechnology:Li-nanomaterials li ka etsa hore ba fihle moetlong oa Valge ho ntlafatsa thepa le sampole ho kenya mekhoa ea ho boloka makoliti.
Sephetho
Lithako tsa ChromatograftE kanna ea utloahala e le karolo e nyane ea sistimi ka kakaretso, empa ba bapala karolo ea bohlokoa ho khethollang ho nepahala le lits'ebetso tsa lits'ebetso tsa karohano ea lik'hemik'hale. Lipuo tsa morao-rao tsa morao-rao tse entsoeng ka boqapi li na le tsona tse rarahaneng, fana ka bafuputsi le indasteri e ntlafalitsoeng boleng ba data, litšenyehelo tse theko e boima le maemo. Ha chromatography e ntse e sebetsa hantle, likhopolo tsena li tiisa hore bafuputsi ba ka tšepa hore ba bona ba ntse ba bala. Ho ntlafatsa ho litabeng tsena tse ncha tsa ciaromatography ke matsete a bohlale a tla patala ka boleng le katleho ea lits'ebetso tsa karohano ea lik'hemik'hale tsa lik'hemik'hale.
Fumana likarabo ho lipotso tse 50 HPpc se ikhethang se ngotsoeng se fumanehang le se rutang, temoso ea bohlokoa: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang
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