Ukulungiswa kwesampulu linyathelo elibalulekileyo kwi-scomatography yeChromatography-Mass Spektrometry (LC-MS), ukuphembelela ukuchaneka kunye nokuthembeka kweziphumo zohlalutyo. Eyona njongo iphambili yamalungiselelo eesampulu kukukhupha, ukuhlambulula, kunye nohlalutyi-nxaxheba abanzima bematriki, ukuqinisekisa ukuba bayahambelana nohlalutyo lwe-LC-MS-ms. Eli nyathelo libalulekile ekupheliseni izithotho ezinokubakho ezinokuthi zichaphazele umgangatho wedatha. Ukulungiswa kwesampulu efanelekileyo kuphucula ubuntununtunu kunye nokuthintelwa kohlalutyo, olu lubalulekile kwizicelo kwimimandla eyahlukeneyo enjengeeProtetics, imetabomics, uhlalutyo lwezendalo, kunye novavanyo lwendalo.

Iinjongo eziphambili zolungiselelo lwesampulu:

Ukususwa kwezithotho: Ukuphelisa izinto ezinokuphazamisa ukufunyanwa kohlalutyi ekujoliswe kuko.

Ukugxininiswa kohlalutyi-kocaphulo: ukwandisa ukugxininisa kohlalutyo ukuphucula imida yokufunyanwa.

Ukuhambelana: Lungisa iisampulu ngendlela ezinokuthi zihlalutywe ngempumelelo ziinkqubo ze-LC-ms.

Ufuna ukwazi ngakumbi malunga nokulungiselela isampulu ye-HPLC, nceda ujonge eli nqaku: I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Iindlela eziqhelekileyo kwi-LC-MS isampulu yesampulu

Iindlela ezininzi zobuchule zihlala zisebenza kwi-LC-Nks yesampulu. Indlela nganye inezibonelelo zayo kwaye ikhethwe ngokusisiseko kwiimfuno ezithile zohlalutyo.

1. Ukukhupha inqanaba lesigaba (Spe)

I-Spell isetyenziselwa ukwezahlula kwiisampulu ezingamanzi. Obu buchule lubandakanya ukugqithisa isampulu ngenqanaba eliqinileyo lokukhetha ukugcina uhlalutyo ekujolise kulo ngelixa uvumela ezinye izinto ezidlulayo. I-Spee isebenza ngokukhethekileyo kwiimatriki ezintsonkothileyo njengegazi okanye umchamo kwaye unike izibonelelo ezininzi:

Amaxabiso aphezulu okubuyisela: isilwanyana sihlala sivelisa amaxabiso aphezulu okubuyisela ekuthintelwe ukuba akhutshwe ngamanzi.

Ukungqinelana okuzenzekelayo: Indlela inokuba lula, ukuphucula ukuqhutywa kunye nokusebenza kakuhle.

I-2. Ubungakanani beProtein

Iproteyini yeProtein yindlela elula kodwa esebenzayo yokususa iiproteni kwiisampulu zebhayoloji. Obu buchule bubaluleke ngakumbi xa behlalutya iisampulu ezinomxholo weprotheni ephezulu, njengeplasma okanye i-serum. Ngeeproterititititititititititititititing, le ndlela yenze lula iMatrix kwaye inciphisa ukuphazamiseka kuhlalutyo olulandelayo.

Ukukhawuleza kunye nokusebenza ngokuchanekileyo: Ivumela ukuqhubekeka kwezimpulu ngelixa kugcinwa amazinga okubuyisela kakuhle.

I-3. Ukukhupha i-windows

I-LLE izahlule ngokusekwe kulwelo lwabo kwizinto ezimbini ezityhutyweni. Obu buchule luluncedo ekukhupheleni kongezolile okanye ezimongaleleyo zepolares kwiimatriki ezintsonkothileyo.

Isicelo esiguquguqukayo: LE SIBUNGAYIQHUTYWA KOMGAQO-NKQUBO WOKUGQIBELA WAMANQAKU NABANTU, usenza ilungele uluhlu olubanzi lwezicelo.

I-4. Ukugxotha

Ukugxothwa kubandakanya ukuguqula imichiza ukuphucula ubuchule babo kuhlalutyo lwe-LC-ms. Le nkqubo iphucula iipropathi ezinje ngokuzinza okuzinzileyo kunye nokusebenza ngokuphumelelayo, iyoyenza ibe luncedo kakhulu kwimichiza enophando olusezantsi lwendalo.

Uvelwano oluphuculweyo: Ukuphuculwa kwengqondo kunokwandisa kakhulu ubuntununtunu kunye nokukhetha uhlalutyo lweeklasi ezithile ezihlanganisiweyo njengemifanekiso ye-amino acid okanye ii-steroids.

Ufuna ukwazi ngakumbi malunga nokusetyenziswa kwe-vialsgraphy ye-vials, nceda ujonge eli nqaku: I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

Izinto ezichaphazela isampulu yendlela yokhetho lwesampulu

Ukukhetha indlela efanelekileyo yokulungiselela i-Sporesment kuqwalaselwa ngezinto ezininzi:

Uhlalutyi-chemistry: Ukuqonda i-polaty, intlawulo, kunye nokuzinza kuhlalutyi kubalulekile.

Isampulu yesampulu kunye nobunzima: Imiqobo yevolumu kunye nobunzima beMatrix yesampula inokuchaza ukuba zeziphi iindlela ezinokwenzeka.

Iimvakalelo zovakalelo: Izicelo ezahlukeneyo zinokuba nemvakalelo yokwahlukanisa iimfuno zokhetho lwempembelelo.

Izixhobo zeLabhoratri: Ukufumaneka kwezixhobo ze-automation okanye ukulungisa i-sumaza kunokuchaphazela ukhetho lwendlela.

Eyona ndlela ilungileyo yokulungiselela isampulu yesampulu

Ukufezekisa iziphumo ezilungileyo kuhlalutyo lwe-LC-MS, ukubambelela kwiindlela ezilungileyo zamalungiselelo emodeli kubalulekile:

Ukubekwa ngokubekwa emgangathweni

Ukuqinisekiswa: Qinisekisa ngeendlela zokuqinisekisa ukuthembeka kwabo kunye nokuchaneka.

Nciphisa ukuphatha isampula: ukunciphisa inani lezinto ezingenamsebenzi ukuthintela ilahleko okanye ungcoliseko.

Sebenzisa i-Regegents yomgangatho: Sebenzisa iirejista eziphezulu kakhulu ukuthintela ukwazisa iisampulu.

IINKQUBO ZESICWANGCISO: Gcina iirekhodi ezineenkcukacha zazo zonke iinkqubo zokwenza lula ukuqinisekiswa kwengxaki kunye nendlela.

Ukuqukumbela

Ukulungiswa kwesampulu yesampulu kubalulekile kuhlalutyo lwe-LC-ms. Ngokuqonda ngeendlela ezahlukeneyo njengeSpecielity, iProtein Primiction, iProtein, kunye nokugxothwa, abahlalutyi banokukhetha eyona ndlela ifanelekileyo kwiimfuno zabo ezithile. Ngaphaya koko, ukulandela ezona ndlela zilungileyo kuya kuphucula umgangatho wedatha kunye nokuqinisekisa iziphumo ezinokuthenjwa kwizicelo ezahlukeneyo kuphando kunye noseto lweshishini.

Ukuqukumbela, iindlela zokulungiselela isampulu yesampulu ayinakuphucula nje ukusebenza kohlalutyo, kodwa kukwenza igalelo elibonakalayo ekuhambeni kweendawo ezifana nezonyango, uphuhliso lweziyobisi, kunye nokuhlolwa kwezesimo.

Vula iimfihlo ze-veromatography ye-veromatographys efanelekileyo yokuhlalutya ngokuchanekileyo nangokuthembekileyo kumanyathelo ama-6 nje alula.Funda ukuze ufumane inkosi ubuchule! Amanyathelo ama-6 okulungiselela i-vialsgraphy ye-vials yohlalutyo

Ngaphambili:

I-LCMS VS GCMS: Yeyiphi indlela elungele uphando lwakho?

Okulandelayo:

Yintoni i-vial vial kunye nokubaluleka kwayo kwi-chromatography?