I-chromatography-mass spectrometry (GC-MS) kunye ne-chromatography-manzi spectrometry (LC-MS) ziindlela ezimbini zohlalutyo ezisetyenziswa ngokubanzi kwiSayensi yeNdalo, iKhuseleko loKhuseleko. Zombini iindlela zenzelwe ukwahlula kwaye zichonge imidaniso kwimixube entsonkothileyo, kodwa imigaqo-siseko yazo, ukusetyenziswa, kunye nezibonelelo zahluke kakhulu. Eli nqaku liza kuphonononga umahluko phakathi kwe-GC-Nks kunye ne-LC-MS ukunceda abaphandi bamisele ukuba yeyiphi na indlela elungele iimfuno zabo ezithile.

Vula iimfihlo ze-veromatography ye-veromatography efanelekileyo yokuhlalutywa ngokuchanekileyo nangokuchanekileyoAmanyathelo ama-6 nje alula. Funda ukuze ufumane inkosi ubuchule!

I-GC-NS kunye ne-LC-NS Ushwameva

I-chromatography-mass spectrometry (GC-ms)

I-GC-MS idibanisa i-chromatography yegesi kunye nobunzima bokuhlalutya ukuhlalutya kunye ne-semivolatile. Kule ndlela, isampulu ifakwe kwaye ithuthwa ngekholam ye-chromatographic yirhasi ye-InErt, ihlala i-Helium. Icandelo le-chromatography yegesi liyahlulahlula amakhonkco ngokusekelwe kubume bokuzinza kwabo kunye nokusebenzisana neSigaba soShishino seKholamu. Emva kokwahlukana, imitya ingeniswa kwi-spectrometer enkulu apho i-iond kunye nomyinge wawo wokuhlawula ohlawula ohlawulelwayo ulinganiswa. Le nkqubo inokuchonga kwaye icime amakhonkco akho kwisampulu.

Izicelo ze-GC-Nks:

Uhlalutyo lwe-Forensic: GC-Nks ngumgangatho weGold yokuchonga iziyobisi kunye neetyhefu kwiisampulu zebhayoloji.

Ukuhlolwa kwezendalo: isetyenziselwa ukubona ukungcolisa kunye nezinto ezinobungozi emoyeni, amanzi kunye nomhlaba.

Ukhuseleko lokutya: I-GC-Nks inokuchonga iintsalela zepisticide kunye nezinye iimveliso zokutya.

Uhlalutyo lwePetrochemical: Kugqityiwe ukuhlalutya iimveliso zepetroleum kunye nezixhobo zabo.

Ngaba ufuna ukwazi ngakumbi malunga nokuba kutheni i-villa ye-Promatoces esetyenziswe kwiChromatography ?, Nceda ujonge le arcece:Kutheni le nto i-villa ye-ERPE isetyenziswa kwiChromatography? Ii-Angles ezili-12

I-Chromatography-Mass Spectrometry (LC-NS)

I-LC-NS idibanisa i-chromatography ye-chromatography ene-spectrometry, iyenza ilungele ukuhlalutya uluhlu olubanzi lwezinto ezibanzi okanye ezingabalulekanga. Kwi-LC-Nks, isampulu ichithwe kwisigaba seselula se-Liqhenqa, esikhutshelwe kwikholamu egcwele iSigaba sokuShiti. Iikhompawundi zahlulwe ngokusekwe kwiipropathi zabo zekhemikhali, kwaye emva kokwahlukana, zingu-Ioswed kwaye zihlalutywa nguSpectrometer omkhulu.

Ukusetyenziswa kwe-LC-Nks:

Uphando ngamayeza: I-LC-ND isetyenziselwa ukukhula kweziyobisi, kubandakanya i-pharmacokinetics kunye nokuchongwa kwemetabolite.

I-Biotechnology: Kubalulekile ekuhlalutyeni iiproteni, i-peptides, kunye ne-acid acids.

UQEQESHO LWEZOBUCHULE: I-LC-NS iqeshwa uhlalutyo lwee-biomarkers kunye namachiza onyango kwiisampulu zeklinikhi.

Uhlalutyo lokusingqongileyo: olufanayo kwi-GC-Nk, i-LC-LC-Nks isetyenziselwa ukufumana amathuko kuma-matriki, kubandakanya namanzi nomhlaba.

Ufuna ukwazi ngakumbi malunga nokusetyenziswa kwe-vialsgraphy ye-vials, nceda ujonge eli nqaku: I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

Umahluko ophambili phakathi kwe-GC-NS kunye ne-LC-NS

1. Inqanaba le-Mobile

Owona mahluko ubalulekileyo phakathi kwe-GC-NS kunye ne-LC-Nks linqanaba leselula elisetyenziselwa ukwahlula. I-GC-ms isebenzisa iSigaba se-GeBugh Paly, siyenza ilungele imilinganiselo ye-willing kunye ne-semi. Ngokwahlukileyo, i-LC-MS isebenzisa isigaba seselula se-Liqhenqa, sivumela uhlalutyo lweentlobo ngeentlobo zemichiza yezinto ezihlanganisiweyo, kubandakanya nezo zingenakuphangwa ngaphandle kokuthambisa.

2. Ukulungiswa kwesampula kunye nokuhambelana

I-GC-ms ifuna iisampulu zibe kwi-sorvent ye-polar kwaye kufuneka ifakwe qho ngaphambi kohlalutyo. Le mfuneko ibeka umda kukwenza isicelo kunye nokudibanisa amanqaku aphezulu kunye nozinzo lobushushu. Kwelinye icala, i-LC-MS inokuhlalutya iisampulu ze-polar kwaye ihambelana nematriki enobunzima, eyenza ilungele uluhlu olubanzi lohlalutyo, kubandakanya i-biomolecules ezinkulu.

I-3. Imvakalelo kunye nemida yokufumana

Zombini iindlela zobugcisa zibonelela ngeemvakalelo eziphezulu, kodwa ukusebenza kwazo kungahluka ngokuxhomekeka kuhlali. I-GC-ms inovelwano ngakumbi ngeempukane, ngelixa i-LC-ms ineemvakalelo ezibalaseleyo kwi-no-faces. I-LC-MS inokufumana imida yokufunyanwa ephantsi yeeklasi ezithile zezinto ezihlanganisiweyo, ezinje ngamayeza nakwiibimolecules.

I-4. Iindleko zokusebenza kunye nobunzima

Iinkqubo ze-GC-ss zihlala zinzima kwaye zifuna uqeqesho oluncinci oluncinci kune-LC-ms. Ngenxa yoko, i-GC-MS inokuba neendleko ngakumbi kwiilebhu ezinemiqobo yohlahlo-lwabiwo mali. I-LC-ms, ngelixa ihambisa izinto ezibanzi, kubandakanya ezinye izinto ezininzi kunye nesondlo, enokwandisa iindleko zomsebenzi.

Ukukhetha ubuchule obufanelekileyo kuphando lwakho

Xa uthatha isigqibo phakathi kwe-GC-Nks no-LC-Nks, abaphandi kufuneka bathathele ingqalelo izinto ezininzi:

Uhlobo lohlalutyo: Ukuba iithayile zethagethi ziguquguqukayo ziguquguqukayo kwaye zizinzile ngoku, i-GC-ms inokuba lukhetho olukhethwayo. Kwimigangatho enkulu, engaqhelekanga, okanye engazinzanga, i-LC-ms ifanelekile ngakumbi.

Isampulu yeMatrix: Ubunzima beMatrix yesampulu bunokuba nefuthe kukhetho lwendlela. I-LC-NS ihlala ibhetele kwiisampulu zebhayoloji, ngelixa i-GC-DS igqwesa kwizicelo zokusingqongileyo kunye nezixhobo ze-forensic.

Iimfuneko zemvakalelo: Ukuba uphando lufuna ukubongozwa koxinzelelo lwezinto ezingezizo ezingezizo ezingezizo, i-LC-MS inokubonelela ngemvakalelo eyimfuneko.

Uhlahlo-lwabiwo mali kunye nezixhobo: Cinga ngeendleko zokusebenza, kubandakanya ulondolozo noqeqesho, xa ukhetha indlela yendlela. I-GC-MS inokuba nento enokwenzeka ngakumbi kwiilebhu ezincinci ezinesibonelelo esinqongopheleyo.

Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ukuqukumbela

Zombini i-GC-Nks kunye ne-LC-Nks zinobuchule onamandla ohlalutyo kunye nezicelo ezizodwa. I-GC-Nks ilungele ukuhlalutya iziqu eziguqukayo kwaye zisetyenziswe ngokubanzi kuhlalutyo lwendalo nolusingqongileyo. Ngokwahlukileyo, i-LC-Nks inomsebenzi obanzi wezinto ezingafakwanga kwaye zenziwe i-labilill kwaye zenze ukuba ingabaluleki kuPhando lweePhezulu kunye neBiolotchnology. Ekugqibeleni, ukhetho oluphakathi kwe-GC-Nks kunye ne-LC-MS kufuneka lusekwe kwiimfuno ezithile zophando, kubandakanya uhlobo lohlalutyo, ubunzulu beMatrix, iimfuno zemvakalelo, kunye nezixhobo ezikhoyo. Ngokuzibandakanya ngononophelo ezi zinto, abaphandi banokukhetha eyona ndlela efanelekileyo yokufumana iziphumo ezichanekileyo nezinokuthenjwa kuhlalutyo lwabo.

Ngaphambili:

I-HPPC vs. Capillary Electrophoreris: Yeyiphi oza kuyisebenzisa?

Okulandelayo:

Yonke into ofuna ukuyazi malunga nokulungiselela isampulu ye-LCMS