I-Vials yokuBuyisa i-HLLC kunye nohlalutyo lwe-GC: Amanqaku ama-5

I-vials yokubuyiselazizinto ezenziwe ngokukodwa iilebhu zenzelwe ukwandisa ukubuyiswa kwesampulu ngexesha lohlalutyo. Ubume babo obukhethekileyo kunye nolwakhiwo lunciphisa isixa sesampulu yentsalela, ukuqinisekisa ukusetyenziswa kwesampulu yesampulu ngexesha lovavanyo. Uninzi lwee-vials eziphezulu zokubuyisela kwimeko yesiqhelo zenziwe ngohlobo lweglasi, eyaziwa ngokuchasene nekhemikhali kunye nozinzo lobushushu. Ezi vils zisetyenziswa ngokubanzi kwimisebenzi yeelebhu efuna ukhathalelo lwesandla esiphezulu sokuchaneka kunye nohlalutyo.

Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

1. Nciphisa ilahleko yesampulu

Enye yezona zinto zibalulekileyo ezichaphazela ukuchaneka kwe-HLLC kunye ne-GC yilahleko yesampuli ngexesha lokuphatha kunye nokudluliselwa. Ii-vials zokubuyisela kwimeko yesiqhelo zenzelwe ngokukodwa ukunciphisa oku kulahlekelwa ngamanqaku abo akhethekileyo:

Uyilo oluqhelekileyo: I-Vials yokuBuyisa iPhezulu ihlala ibonisa indlela enamanzi okanye yoyilo lokwandisa ukubuyiswa kwesampulu. Olu yilo luncitshisela ivolumu yentsalela, ngaloo ndlela kunyusa isampulu.

Ukuvulwa okukhulu: Ezinye ii-vill ezinkulu zokubuyisela kwimeko yesiqhelo zinokuvulwa okukhulu ukuqhuba ukusetyenziswa kwezirinji okanye imibhobho, kuvumeleke ukuba kukhutshwe isampula. Oku kunciphisa umngcipheko wezinto ezigqithisileyo kwi-vial, ngaloo ndlela kuphephe ukungachaneki.

2. Ukuphucula ingqibelelo yesampulu

Ukugcina ingqibelelo yesampulu kubalulekile kwi-HLLC echanekileyo kunye ne-GC. I-vials yokubuyisela iphakamileyo iphumeza le kwezi ndlela zilandelayo:

Ukuxhathisa iikhemikhali: Ezi vils ziqhele okwenziwa zizixhobo ezisemgangathweni, ezinjengeglasi, ekwakuxhathisa ukuphendula kwamachiza okunokutshintsha ukwakhiwa kweendlela zokuzonwabisa. Oku kuxhathisa kunceda ekuqinisekiseni ukuba iisampuli zihlala zisebenza ngexesha lokugcina kunye nohlalutyo, zibangela iziphumo ezinokuthenjwa.

I-Airtight Inketho yokhetho: I-Vials ye-Vials ePhakamileyo ihlala ingena kunye neenketho zetywina le-airtight, ezinjengeI-crmimp capsokanyeI-CAPS CAPS, ekuthinteleni ungcoliseko oluphumayo kunye nolwangaphandle. Oku kubaluleke kakhulu kwiinkampani eziguquguqukayo zihlaluthwe yi-chromatography yegesi, apho iinguqu ezincinci kuxinzelelo zinokuchaphazela iziphumo.

3. Ukuphucula ukuzala kwakhona

Ukuzala kwakhona lilitye lembombo lokuchaneka kohlalutyo. Ii-Vials zokubuyisela eziPhezulu zoncedo zinceda ukuphucula ukuzala ngeendlela ezininzi:

Ivolumu yesampulu engaguqukiyo: ngokunciphisa i-Dereover, i-vials yokubuyisela ePhakamileyo iqinisekisa ukuba ivolumu yesandla engaguqukiyo isetyenziselwa uhlalutyo ngalunye. Oku kungqinelana kubalulekile ekuthelekiseni iziphumo ngeendlela eziqhuba kakhulu okanye iimvavanyo, apho izahluli kwi-paysiations ikhokelela kulwahluko olubonakalayo kwidatha.

Iimeko ezisemgangathweni Oku kumiselweyo kunciphisa ukungafani okubangelwe ngumahluko ekusebenzeni kwe-Vial, kukhokelela kwiziphumo ezinokuthenjwa kunye nokuzala.

I-4. Ukuhambelana neenkqubo ezenzekelayo

Kwilebhu yale mihla, i-akes idlala indima ephambili ekuphuculeni ukusebenza nokuchaneka. I-Vials yokuBuyisa i-Vials ihlala ihambelana neenkqubo zesampulu ezisetyenziswayo ezisetyenzisiweyo kwi-HPLC ne-GC:

Ukudityaniswa kwe-wamp: I-Vials yokubuyisela iPhezulu yenzelwe ukulingana ngeendlela ezizenzekelayo ngaphandle kokulungiswa kakhulu. Oku kuyahambelana nokuqinisekisa ukuba iisampuli ziqwalaselwa ngokukhawuleza kwaye ngokuchanekileyo, ukunciphisa iimpazamo zabantu ezinxulumene nokulungiswa kwencwadi.

Ukuncitshiswa kweempazamo zokulungisa: Ukuzenzekelayo kunciphisa ungenelelo lwabantu ngexesha lokulungiselela kwesampulu kunye nohlalutyo, ukunciphisa isimo sempazamo ezinokuchaphazela ukuchaneka kwezimpazamo ezinokuchaphazela ukuchaneka kwezimpazamo ezinokuchaphazela ukuchaneka kweempazamo ezinokuchaphazela ukuchaneka kweempazamo ezinokuchaphazela ukuchaneka kweempazamo ezinokuchaphazela ukuchaneka kweempazamo ezinokuchaphazela ukuchaneka. I-Vials yokubuyisela iPhezulu iqhubela phambili le nkqubo ngokuqinisekisa ukuba iisampulu zifunyanwe ngokufanelekileyo kwaye zihlaziywe ngelixa zinciphisa umngcipheko wokulahleka.

I-5. Ukuphuculwa kokuhlalutya

Ukuncitshiswa kwelahleko yesampulu, ukunyuka okunyukayo, kunye nokuphuculwa kokuzalwa ngokutsha kokuphucula ukusebenza kohlalutyo:

Imvakalelo ephezulu: ngokukhulisa ukuphinda ibuyise isampula, i-vials yokubuyisela ePhakamileyo inceda ukuphucula ubuntununtunu bemvakalelo ye-HLLC kunye ne-GC. Ukwanda kwengqondo kubalulekile ekufumaneni ugxininiso olusezantsi lohlalutyi, oluhlala lufuneka kwimihlaba efana namayeza okusingqongileyo kunye nokuhlolwa kwendalo.

Isisombululo esiphezulu: Kwizicelo ze-GC, amandla okugcina ingqibelelo yesampulu iphucula ukusonjululwa phakathi kweencopho zeChromatographic. Isisombululo esiphuculweyo, imiqathango kwimixokelelwane entsonkothileyo inokuchongwa ngokuchanekileyo kwaye ilungiswe.

Ufuna ukwazi ngakumbi malunga nokubuyiselwa kwe-HPLC ephezulu, nceda ujonge eli nqaku:Ukubuyiselwa kwe-HLLC ephezulu ye-HLLC

Njengoko iimfuno zohlalutyo ziyaqhubeka zitshintsha ngokwandisa ubunzima kunye neemfuno zolawulo, i-Vials yokuBuyisa i-Vials sisixhobo esisebenzayo sohlalutyo lwe-HPRC kunye negesi chromatography. Ayisiyo kuphela iqinisekile imilinganiselo echanekileyo, banokunceda uphando lwenzululwazi kunye nohlalutyo kumashishini anje ngamayeza, isayensi yezendalo esingqongileyo, ukhuseleko lokutya, nangaphezulu.