Yeyiphi i-HPLC ilungele ukuhlaziywa kwe-UV-?

Intsebenzo ephezulu ye-chromatography (i-HPPC) yindlela esetyenziswayo ngokubanzi yokuhlalutya ngokwahlula-hlula, ukuchonga, kunye nezinto ezithile kumxube. Kwabacimi abahlukeneyo abakhoyo kwi-HPLC, i-UV-Vis (i-UV-Vis) yeyona ixhaphakileyo ngenxa yokusetyenziswa kwabo okusetyenziswayo, ubuntu obuphezulu, kunye nokukwazi ukuhlalutya uluhlu olubanzi lweendawo ezibanzi. Eli nqaku lifungela iintlobo ezahlukeneyo ze-UV-Vis Read Backago, indlela abasebenza ngayo, izibonelelo zabo, kunye nokuqwalaselwa kwabo ukukhetha.

Iindidi ze-UV-Vis

I-1. I-UV-Vincy Vid Ark

Oovimba abangenantlonelo bavumela imilinganiselo ukuba ithathwe kwindawo enye okanye engaphezulu. Ngokwesiqhelo babeka kwindawo eqhelekileyo yamaza, enje nge-254 NM yezinto eziphilayo. Ezi zixhobo ziluncedo kwizicelo ezijolise ekuhlaleni.

I-2. I-Photodiode Array (PDA) I-CARD

I-PDA SECTAPAYINGAINGAYIZA I-SPECT REAVETHWANTES ZEXESHA LENXALENYE LAMNQOPHISO ngaxeshanye, elinokuphucula uvakalelo kunye nesantya kuhlalutyo olufuna amaza amaninzi. Eli nqaku livumela uvavanyo olungcono kunye nokudityaniswa kweekhompawundi ezisekwe kwi-surture eyohlukileyo.

I-3. Diode Array Critct (Tata)

Ifana ne-PDAs, ootata abamba i-vectrium epheleleyo ngaphezulu koluhlu lwamagqabi ngexesha lokwenyani. Eli nqaku liluncedo kakhulu kwimixokelelwane entsonkothileyo apho ii-comples ezininzi zinokuba phezu kwe-elute ngaxeshanye. Utata unokubonelela ngolwazi oluthe kratya malunga nokucoceka kwe-Peiki kwaye ancede echonge izinto ezingaziwayo ngokusekwe kwiiprofayili zabo ezibonakalayo.

Imigaqo yokusebenza

I-UV-Vis Reciads isebenza ngokudlulisa i-UV kunye nokukhanya okubonakalayo ngemodeli kwiseli yokuhamba. Ngokulinganisa isixa sokukhanya esinxunguphele ngesampulu kumaza awahlukeneyo, uxinzelelo kunye nokubhalwa kwabavavanyi kunokuqondwa.

Izinto eziphambili:

Light source: Typically a deuterium lamp is used for UV detection (190-400 nm) and may include a tungsten lamp for visible detection (400-800 nm). Ezinye iinkqubo eziphambili zisebenzisa i-Xenon Lamps ukugubungela zombini i-ranges yokunyusa ukusebenza kakuhle.

Iseli yokuhamba: Uyilo kunye nomthamo weseli yokuhamba ubalulekile kwimvakalelo. Iiseli ezincinci zokuhamba zinokuphucula imida yokufunyanwa kodwa inokuzisa ukongeza kwi-band.

Inkqubo yokufumanisa: Umcingeli uguqula utshintsho kubunzulu bokukhanya kumqondiso wombane ohambelana noxinzelelo lohlalutyo kwisampulu.


Ufuna ukwazi ngakumbi malunga nokulungiselela isampulu ye-HPLC, nceda ujonge eli nqaku:I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Izibonelelo ze-UV-Vis

Imvakalelo: I-UV-VICTACSINCICINGUCINCINCINAYANOSUYUYELA KULUNGELELANYANYU KUFUNEKA UTSHINTSHE UTSHINTSHO OLUFUMANELEKILEYO kwaye ngenxa yoko banokufumana ubuchule obuphantsi bahlalutya.

Ukusebenza

Uhlalutyo olungathethekiyo: Inkqubo ayitshintshi okanye itshabalalise isampulu, ke yohlalutyo lunokwenziwa njengoko kufuneka.

Uluhlu olubanzi: ukukwazi ukuhlalutya uluhlu olubanzi lweekhontrakthi kunye ne-chromophores (amaqela afunqileyo), alungele izicelo ezahlukeneyo.

Ukuqwalaselwa xa ukhetha i-UV-Vis Strictor

I-1. Uluhlu lwe-waveleler

Ukukhetha iintlobo ezahlukeneyo zemithombo yokukhanya (deberium, i-tungsten, kunye ne-xenon) kuchaphazela i-wavelength yoluhlu olunokusetyenziselwa ukufumanisa. Ngohlalutyo olubanzi, ngakumbi uhlalutyo lwemixube entsonkothileyo, i-waveled wavelength avelength ikhetha.

2. Uhlobo lokufumanisa

Ukukhetha phakathi komncili ongenakwenziwa kunye ne-PDA \ / utata uxhomekeke kwiimfuno ezithile zohlalutyo:

Umnxibelelanisi ongenakuthintelwa: Eyona nto ilunge kakhulu kuhlalutyo ekujoliswe kulo apho kwaziwa i-wavelength ekhethekileyo.

I-PDA \ / Tata: Ilungele iisampulu ezintsonkothileyo ezifuna uhlalutyo lwangaphambi kwexesha.

I-3. Ukuhamba kweseli

Uyilo luchaphazela ubuntununtunu kunye nesisombululo:

Iseli yokuhamba kancinci iphucula imvakalelo kodwa inokubangela ukusasazeka.

Ubude bendlela bukwabalulekile; Indlela ende yendlela iphucula imilinganiselo ye-abtorbey kodwa ishukumayo.

4.Ukusetyenziswa kokusetyenziswa kunye nokudityaniswa

Ukuhambelana neenkqubo ezikhoyo ze-HLLC kunye nonxibelelaniso olunabasebenzisi lunokufumana impembelelo yokusebenza kwelebhu.

Izicelo ze-UV-Vis

I-UV-Vis Reaces ibalulekile kwiinkalo ezahlukeneyo:

Amayeza: Isetyenziselwa ukulawulwa komgangatho kunye nohlalutyo lwe-infring.

Ukuvavanywa kokusingqongileyo: Ukubeka iliso kwi-ngcordants kwiisampulu zamanzi.

Umzi-mveliso wokutya: Ukuhlalutya ukongezwa kunye nokungcola.

I-Biotechnology: Ukuvavanya ukugxininisa kwiinkqubo zebhayoloji.

Ufuna ukwazi ngakumbi malunga nokusetyenziswa kwe-vialsgraphy ye-vials, nceda ujonge eli nqaku:I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

Ukukhetha owona mboniso we-HPLC obalaseleyo wohlalutyo lwe-UV-Vis kufuna ukuqonda iimfuno ezithile zesicelo, kubandakanya iimfuno zovakalelo, udidi lwe-wavelele, kunye neempawu zesampulu. Ngelixa i-recin enxunguphele ilungele ukuhlalutya okulula, i-PDA noTata noTata banikezela ngeengenelo ezibalulekileyo ngokubhekisele kubunzima kunye nokungaguquki, okubenza balungele imisebenzi yohlalutyo olunomngeni ngakumbi.