I-Autosmilers idlala indima ebalulekileyo kwiilebhu zanamhlanje zelebhu ngokuzenzekelayo yenkqubo yengeniso yentshayelelo kunye nokubonelela ngenkqubo yentshayelelo ezenzekelayo. Inkalo ebalulekileyo koku kukulayisha ngokufanelekileyo iisampulu zibeI-autosampler vialskunye nelungiselelo lesampulu efanelekileyo; Zombini imisebenzi ineempawu ezibalulekileyo kumgangatho kunye nokuthembeka kweziphumo zohlalutyo. Sibeka apha amanyathelo afanelekileyo, iinkqubo ezilungileyo, kunye nezicwangciso zokuqinisekisa ukusebenza kakuhle ngexeshaUkulungiswa kwesampulu\ / iinkqubo zokulayisha ngokuhambelana ne-autosamplers.

Icandelo loku-1: Ukulungiselela ukulayisha iisampulu

Ngaphambi kokuqala komthwalo wesampulu, kubalulekile ukuba wenze imiqathango efanelekileyo. Nantsi into ekufuneka yenzeke:

1.1 Qokelela izixhobo zakho

Qala ngokuqokelela zonke izixhobo ezifanelekileyo kunye nezixhobo:

I-autosampler vials: Khetha i-vials ehambelana ne-autosampler yakho kunye nohlobo lohlalutyo-uninzi lwee-autosamplers Sebenzisa iglasi okanye i-vials yeplastiki kulo msebenzi.

I-CAPS kunye neSepta: Ngonyuselo oluphezulu nongcoliseko, qinisekisa i-CAPS efanelekileyo kunye ne-sepga ehambelana ngokuqinileyo ukuthintela ukuvuza kunye nongcoliseko.

Izixhobo zesampulu: Zilungiselele ngemibhobho, amaringes kunye nezinye iziqwenga ezifunekayo zezixhobo xa kuziwa kwiSampuliP. Oku kunokubandakanya imibhobho, isirinji kunye nezixhobo zesixhobo ukuba ziyasebenza.

Gcina indawo yokusebenza ecocekileyo ukunciphisa umngcipheko wongcoliseko.

1.2 Faka iileyibheli ii-vials zakho

Ngaphambi kokuqala kohlalutyo, i-vial nganye kufuneka ifakwe ilebheli ngolwazi oluphambili kubandakanya isampulu, umhla kunye nazo naziphi na iinkcukacha eziphezulu. Ilebheli iqinisekisa ukulandelela nokulandelela kunye nokuthintela umxube ngexesha lohlalutyo.

I-1.3.handle iisampulu ngononophelo

Faka ngononophelo ukuphatha iisampulu ukwenzela ukuthintela ungcoliseko kunye nelahleko. Sebenzisa izixhobo ezifanelekileyo, ezinje ngemibhobho okanye izirinji, xa udlulisa iisampulu kwii-victs; Kulumkele ukuchukumisa abanye abantu okanye i-vials ye-vials ngeminwe njengoko oku kunokwazisa i-oils kunye nongcoliseko olunokutshintsha iziphumo.

1.4.Qinisekisa ivolumu yesandla esichanekileyo

Ithamsan echanekileyo ibalulekile ekufezekisweni iziphumo ezinokuthenjwa. Sebenzisa izixhobo ezinobunkunkqele ukulinganisa ngokuchanekileyo kwaye ugqithise ivolumu efanelekileyo kwii-vials; Soloko uhleli ngohlalutyo lwendlela yakho yohlalutyo.

1.5 nciphisa iiblubhu zomoya

Iityhubhu zomoya zinokwenza isampulu yesampuluni ichanekile; ukunciphisa ukumiswa kwabo:

Kulumkele ukukhupha iisampulu kwi-vial. Gcwalisa kancinci kwaye ucocekile, ngelixa ucofa ngobunono ukuze afumane amaqamza omoya.

Nje ukuba iisampulu zilayishwe kwii-vials, zitywine ngokukhuselekileyo usebenzisa i-caps efanelekileyo kunye neSepta. Ukufezekisa itywina le-airtight kuya kunceda ukukhusela isampulu okanye ungcoliseko; Xa usebenzisa i-CRIMP CAPS Sebenzisa isixhobo ngokukodwa kuloo msebenzi ukuvalwa okukhuselekileyo.

Khangela ulwazi olunzulu kwi-HPLC yesampulu yesampulu yeli nqaku linye:I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Icandelo 2: Iindlela zeSampulukwanga

Ukulungiswa kwesampulu efanelekileyo kuhlala kubalulekile kuhlalutyo oluchanekileyo. Kuxhomekeka kwindlela yakho yohlalutyo, iisampulu zinokufuna ukuba zitshiswe, zichithwe okanye kungenjalo zikhutshelwe kwi-allsambul vials Vials - Qiniseka ukuba ulandele ezinye iindlela zokuthintela iimpazamo ngexesha lenkqubo.

ICandelo 3: Lasha iisampulu kwi-autosampler vials

Ngoku ukuba iisampulu zakho zilungisiwe, lixesha lokuba ziyifake ngokucwangcisiweyo kwi-autasampler vials:

3.1 Gcina i-vials ngokufanelekileyo

Ukuba uhlalutyo lwakho lufuna ixesha elininzi lokulinda ngaphambi kwenaliti, qinisekisa ukuba ii-vials zigcinwa phantsi kweemeko ezifanelekileyo-umzekelo ukhuseleko lokufumana isampulu kunye nezikhokelo zokugcina inxenye yesampulu yesampulu.

3.2 Cwangcisa i-vials yakho

Seka inkqubo esebenzayo yokulungiselela i-vials yakho. Cwangcisa ngokusengqiqweni, kwaye uqwalasele ukusebenzisa iiracks ze-vial okanye itreyi ukuthintela ukuxuba ngexesha lohlalutyo.

3.3 Yenza itshekhi yokufaneleka

Phambi kokubaleka iisampulu, kuyacetyiswa ukuba uqhube itsheki yokufaneleka kwenkqubo. Oku kuqinisekisa ukuba i-autosampler iyasebenza njengoko icwangcisiwe kwaye iisampulu zakho zilayishwe ngokuchanekileyo.

3.4 Isixhobo soVavanyo loVavanyo lokuLungisa (VCT)

Iisampulu ezahlukeneyo zifuna i-vials ethile; i-amber okanyecacisa i-vialsInganele ngelixa i-vials ekhethekileyo eyenzelwe ngokukodwa izicelo ezithile zinokuba yimfuneko. Kububulumko ukuqinisekisa ukungqinelana kwee-victs zakho kunye nohlobo lwesampulu kunye nendlela yokuhlalutya ukwenzela ukuthintela imiba enokubakho ngexesha lohlalutyo.

ICandelo 4: Umthwalo weposi kunye noHlaziyo

4.1 Gcina kwaye ucoceke kwaye ucoce izinto ze-autosampler

Ukulondolozwa kokusebenza okungaguqukiyo kwe-andesampler, ukugcina rhoqo nokucoca izinto zayo-kubandakanya neesampulu, izirinji kunye nezinamathi ezinamanqwanqwa. Intsalela okanye i-ungcoliseko inokuba nefuthe elinokuba yimpembelelo kwisampulu ekhokelela kwiziphumo ezingachanekanga; Ukulandela izindululo zomenzi kunye nokuseka ishedyuli yolondolozo.

4.2 Uxwebhu lwenkqubo yakho

Inkalo ephambili yomsebenzi welebhu kukugcina amaxwebhu acokisekileyo. Qiniseka ukuba ubandakanya nakuphi na ukuphambuka okanye imiba edibene nayo ngexesha lenkqubo yokulayisha kwesampulu kolu xwebhu, oluya kusebenza njengomthombo obalulekileyo wokulungisa ingxaki yokuhlalutya.

4.3 Iitsheki zoQinisekiso lweMgangathweni kufuneka ziphunyezwe

Ukumiliselwa kwenkqubo efanelekileyo yokulawula umgangatho (i-QC) yokuvavanya ukusebenza kwe-autosampler kunye nokuchaneka komhlali, enokubandakanya imigangatho eyaziwayo, enokubandakanya imigangatho eyaziwayo, i-vesks kwaye iphinde iphinde ichaneka kokuchaneka nangokuchanekileyo. Nakuphi na ukuphambuka kwiziphumo ezilindelweyo kufuneka kuqhubeke kwakhona uphando ngakumbi kunye nenyathelo lokulungisa ukuthathwa kwangoko.

4.4 Sombulula imicimbi ngokukhawuleza

Ukucombulula iingxaki okanye iziphumo ezingahambelaniyo ngokukhawuleza kubalulekile ukuba zivela, ezinjengeengxaki zokulayisha okungalindelekanga ezinjengokuncamathisela okanye ukungcolisa ukungathobeki umgangatho wedatha. Ngokuthatha indlela yokucwangciswa kwengxaki, iingxaki ziya kuchongwa ngokulula kwaye zilungiswe ngokukhawuleza.

Ukulungiselela ubugcisa bookulungiselela isampulu kunye nokulayisha i-vials ye-autosampler yeyona nto iphambili ekuveliseni iziphumo ezichanekileyo nezinokuthenjwa. Ngokuthobela ezona ndlela zilungileyo, ukugcina izixhobo kunye nokubeka iliso kwimicimbi enokubakho, unokuqinisekisa ukuba iisampulu zakho zilayishwe ngokuchanekileyo nangokungaguquguqukiyo ekuqinisekiseni impumelelo yakho yophando okanye ukubonelela ngedatha ethembekileyo yophando okanye ukulawula umgangatho.

Fumana iimpendulo kwimibuzo engama-50 exhaphakileyo ye-HPLC ye-HPLC kwinqaku elibanzi nelifundisayo:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Izizathu ezi-13 eziphambili zokusebenzisana nenkampani yethu ye-chromatography

Okulandelayo:

Yintoni i-vial vial vial