Kwi-HPLC yesampulu yokulungiselela, iifilitha zesirinji zidlala indima ebalulekileyo. Iinkcukacha kwiisampulu zinokufumana iikholamu ze-clog, zinciphise iikholamu zoBomi, iBandlazezazezazezezezeilize Clitction Stoctor, kunye nokuzala kwakhona. I-pre-fallication isusa le nto yokungcola, ukuphucula ukuphindaphinda kunye nokuzinza kwesiseko. Izifiso zenaliti zesirinji zibonelela ngesisombululo esisebenzayo esingasebenziyo, qinisekisa iisampulu zicocekile ngaphambi kwenaliti, ukukhusela ikholamu kunye nesixhobo, kunye nokuphucula ukuthembeka kohlaluko

Iimpawu eziphambili zeZixhobo ezahlukeneyo ze-membrane

I-ptfe (iPolytetrafluoroothylene) I-membranes

Ukuhambelana kwamachili:I-Intert engaqhelekanga kwaye ixhathise phantse kuzo zonke izinyibilikisi zezinto eziphilayo, i-AIDS, kunye neziseko, kodwa zingafanelekanga ukuba ziphinde zenziwe ngokufihlakeleyo. Ilungele izinyibilikisi eziphezulu (i.G., I-Acetostitrile, i-DMSO), amaqondo obushushu aphezulu, kunye neemeko zoncedo. Ukukhupha phantse akunakukhutshwa.
Umgangatho wohambo:Ubume obuncinci bufuna imanzi ngaphambi kwexesha kunye ne-organic yeSolvent, ikhokelela kumazinga aphantsi okuhamba.
Iprotheyini yokubopha: Umphezulu we-hydrophobic uyivelisa i-Adsorvoin engalunganga yeproteyini, esenza ilungele ukuhlalutya kwinqanaba.Sebenzisa iimeko kunye nokusikelwa umda:Eyona nto ilunge kakhulu kwi-harsh okanye iisampulu ze-polar ze-polar zepoli (diaid digs, i-Reagent). Hayi amanzi ngaphandle kokumanzisa ngaphambi kwexesha; Khusela i-membrane yomisa ukunqanda iinyembezi.

I-nylon membranes
Ukuhambelana kwamachili:I-hydrophilic; Ihambelana namanzi kwaye uninzi lwezinyibilikisi eziphilayo. Iinyikima eziqhelekileyo ezinje nge-Acetostitrile kunye ne-DMSSO zixhaswa ngokupheleleyo, ngelixa i-STA kunye ne-methanol ephezulu ziphantsi kolumkiso. Ukuzinza kwehla kwi-Acids eqinileyo \ / iziseko.Umgangatho wohambo: I-Pemorate-bift-bit kunye neefilitha ngokukhawuleza kune-ptfe, kodwa ihamba kancinci kune-pvdf.Iprotheyini yokubopha:I-adserction ephezulu engafanelekanga-ingafanelekanga ukuba iisampulu ezityebileyo zeprotheyini ezifuna ukuchacha. Sebenzisa iimeko kunye nokusikelwa umda: I-switticaile ye-organic ye-organic yeSolvent okanye iisampulu ezinamanzi (E.G., Acetonitrile, i-methanol). Icinyiwe kwaye izenzekelayo, kodwa uphendule iprotein okanye i-acid-acid \ // iisampulu zesiseko.

I-PVDF (Polyvinylidene Flooride) I-membranes
Ukuhambelana kwamachili:I-hydrophilic; Izinzile ngamanzi, i-alcohols, kunye neeKetones ezingenakutshutshiswa ngobumnene kwi-acidicly \ / iimeko ezisisiseko. Eyona nto iphantsi kakhulu ye-UV inciphisa uphazamiso lwasemva.Umgangatho wohambo: I-High-Its ngokulula, ukuhambisa i-fills ekhawulezayo kune-ptfey kwi-pore efanayoIprotheyini yokubopha:Ingaphezulu kakhulu kwiisampulu zebhayoloji, iiproteni, kunye ne-Intuth-dolophu ye-sel.
Sebenzisa iimeko kunye nokusikelwa umda:Ilungele iProtein \ / Pillade okanye isampulu yesampulu. Inomdla kwiziseko ezomeleleyo, ezinokuthi zinciphise i-membrane.

I-PC (i-polycarbonate) i-membranes
Ukuhambelana kwamachili: Umhlaba ogudileyo, umphezulu ohambelana nee-acids ezininzi, iityuwa, ioyile, kunye ne-hydrocarbons ezigcweleyo. Ukungaxhathisi i-methanol, i-ketones, ii-enion, okanye i-aromatics.Umgangatho wohambo:I-Iyunifomu eninzi kakhulu ibonisa ukubuyela umva kunye nokuhamba ngokukhawuleza.Iprotheyini yokubopha:Eyona ndawo iphantsi yeglasi engaphantsi ithintele i-adsorvo yokufumana i-adsorvo yeefomathi kunye neemolekyuli ezinkulu.
Sebenzisa iimeko kunye nokusikelwa umda:Ifanelekile ukubalwa kwentsumpa, uhlalutyo lwe-microscopic, kwaye izifundo zeseli zifuna ukugcinwa ngokupheleleyo. Kunqabile ukuba isetyenziswe kwi-organic ye-organic ye-hplic ngenxa yokunqongophala kokucombulula.

Ukuthelekisa intsebenzo

Membrane	Ukuhambelana kwamachili	Umgangatho wohambo	Iprotheyini yokubopha	Izicelo kunye nemida
Ptfe	Ukunganyangeki phantse kuzo zonke izinyibilikisi; hydrophobic	Iphantsi	Isezantsi kakhulu	Izigaba eziphilayo ezihlekisayo; Ayisiyo i-aceus ngaphandle kwe-pre-emanzi
Nylon	I-hydrophilic; Kulungile nge-ACN, DMO; Isilumkiso nge-thf, meoh	Ngokulinganiselweyo	Phezulu	Iinkqubo ze-organics ze-organics \ / buffers; Kulumkele iiproteni kunye ne-asidi eyomeleleyo
I-pvdf	I-hydrophilic; Izinzile ngamanzi, i-alcohols, eKetones	Phezulu	Iphantsi	Iisampulu zebhayoloji Kulumkele iziseko ezomeleleyo
I-PC	Egudileyo; Ilungile ngeeasids, izilonda, ioyile; hayi i-meoh, eKetones	Phezulu kakhulu	Isezantsi kakhulu	Isuntswana \ / microscopy; hayi i-organic ye-organic hplc

Ukukhetha i-membrane elungileyo yesampulu yakho

I-Harsh okanye isinyikithi se-chieffy esiphakamileyo SebenzisaPtfeUkuchasana nekhemikhali engafakwanga kunye nezixhobo eziphantsi.

Iisampulu zebhayoloji (iiproteni) + zitywala \ / amanzi: SebenzisaI-pvdfIprotein encinci yokubopha kunye nokulungelelaniswa okubanzi.
Izinyibilikisi eziqhelekileyo:SebenzisaNylonUkungqinelana ngokubanzi kunye nokuqina-kuyaphephe iproteyini okanye i-ph.
I-sucricle ibala \ / microscopy: SebenzisaI-PCUbungakanani bobuqili obuchanekileyo kunye ne-adsunffiction.

Phawula:Soloko uqwalasela i-chemistry yesampula, iindleko, kunye neemfuno zesixhobo xa usenza ukhetho lwakho lokugqibela.

I-Aijiren ibonelela ngoluhlu olupheleleyo lweefilithi ezisemgangathweni eziphezulu nge-ptfe, inylon, i-PVDF, kunye ne-PCMRNES ukuze idibane nazo zonke iimfuno ze-HPLCs zeSigaba se-HPLC. Nxibelelana nathi kwiisampulu okanye uthethathethwano lobuchwephesha, kwaye masincedise ukukhetha isisombululo secebo lokucoca ulwelo.

Ngaphambili:

I-Vials ye-Adsorction, unyango lwe-vial veres, i-vials ye-SILADE, i-vials edityanisiweyo, i-PFDCs

Okulandelayo:

Kakhulu \ / ubushushu obuphantsi kunye nefuthe lokubonisa ukukhanya kwisampulu yozinzo: Ithiyori kunye nePhambilo