I-chromatography yimpembelelo yokuhlalutya impembelelo isetyenziswe ngokubanzi uqeqesho lwenzululwazi, ukusuka kwi-chemistry kunye namayeza kuhlalutyo lokusingqongileyo. Intembeko ye-vial ving ngexesha lohlalutyo lweChromatography lubalulekile ukuze ufumane iziphumo ezichanekileyo nezichanekileyo; Apha sivavanya iingcebiso ezibalulekileyo ekugcineni ingqibelelo yokufumana iimvavanyo eziphuculweyo nge-chromatography.

I-1.Kiase i-vials esemgangathweni ephezulu

Into ephambili yentembeko ye-vial ilele ekukhetheniI-vials ephezulu. Jonga izixhobo ezaziwa ngokuba ziingenelo zabo ezinje ngeglasi ye-borossilicate okanye i-Inert Plastoplene efana ne-polyproplene engayi kunxibelelana nesampulu yakho, ukuthintela ungcoliseko olunokubakho. Qinisekisa ukuba banemilinganiselo engaguqukiyo yokusetyenziswa kunye ne-autosamplers kunye nezinye izixhobo zeChromatography.

Ukuhlolwa kweziphene

Ngaphambi kokusebenzisa i-vials, yenza uvavanyo olubanzi lokufumana nayiphi na imingxunya, iitshipsi, okanye ukungahambi kakuhle kwiintamo zabo ezinokubangela ukuvuza, ukungcola, okanye ukulahleka kwesampulu ngexesha lohlalutyo. Hlala ulahla i-vials yeyona nto ibalulekileyo ukuze ithande ezintsha ukuze igcine ingqibelelo yesampulu.

I-3.I-CAPS esemgangathweni ye-CAPS kunye neSepta

I-CAPS kunye neSeptazizinto ezibalulekileyo zokugcina ingqibelelo. Khetha ii-caps ezikumgangatho ophakathi kunye ne-sepga yenzelwe ngokuthe ngqo ubuchule bakho beChromatography-yiba yi-chromatography yegesi (i-GC) okanye i-chromatography (LC). Itywina eliqinileyo kufuneka ligcinwe ukuthintela ukuvela, ukungcola okanye ukuphulukana nobugcisa obuguqukayo.

4. I-capper cap yokuqinisa

I-CAP ekhanyise kakuhle i-CAPS ibaluleke kakhulu ukuze ithintele ukuvuza kunye nongcoliseko, ukusebenzisa i-Torque Wrench okanye i-crumirper ye-Torque iya kuqinisekisa iyunifomu kunye nokuqina okungaguqukiyo; Ukutshintsha kwesongezelelo kunokuwonakalisa ii-vials ngelixa uqinisekisa ukuba isiphumo sinokukhokelela kwisampulu yesampula okanye ivuza.

5.Main Imeko ecocekileyo

Indawo ecocekileyo yelebhu iphambili ekunciphiseni umngcipheko wokungcola. Gcina zonke iindawo zokugcina iindawo zokugcina, iindawo zokusebenza kunye nezixhobo ezingenalo uthuli, ubunkunkqele kunye neentsalela zekhemikhali ukwenzela ukugcina ingqibelelo ye-vial ngelixa kwangaxeshanye kuqinisekisa umgangatho wohlalutyo ngelixa kwangaxeshanye uqinisekisa umgangatho wohlalutyo.

6.Store Vials ngokuchanekileyo

Ukugcinwa ngokufanelekileyo kubalulekile ekukhuseleni ukuthembeka kweevils. Beka kwindawo epholileyo, eyomileyo kude nokukhanya kwelanga ngqo kunye nokuguquguquka kobushushu obugqithisileyo ngenxa yeziphumo ezilungileyo. Ngokufanelekileyo, yenza iKhabhinethi okanye indawo ezinikezelweyo ukuthintela umonakalo wengozi ngelixa ugcina i-vials ecocekileyo, engacocekanga.

7.Sebenzisa ukuphathwa gadalala

Ukuphatha ngononophelo ukuphatha ngokubonakalayo kwe-vial vial kubalulekile ekugcineni ingqibelelo yabo. Kulumkele ukuphathwa gadalala kwaye usebenzise izixhobo ezinjengeendlela okanye iitreyi ze-val ukunciphisa ungcoliseko ukusuka kwi-oils yesikhumba kunye nethambeka kwingqibelelo yabo.

Ndinomdla wokufumana izicelo ezahlukeneyo ze-vialsgraphycy? Phonononga eli nqaku lifundise ngokuqonda ukuba lisebenzise i-15:I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

I-8mplemente iProtokholi yokuphamba

Dala kwaye ulandele i-progratction yesandla esebenzayo yokuhambisa iilebheli, ukugcinwa kunye nokulandela iisampulu. Faka ileyibhile yonke i-vials enedatha ebalulekileyo malunga namagama eesampulu, imihla kunye nokugxininiswa kokuthintela iimpazamo zokungabuyiseli.

9.Check ukuvuza

Ngaphambi nasemva kohlalutyo, kubalulekile ukuba uhlole i-vials yokuvuza. Jonga i-septa yazo kunye ne-caps yabo yeempawu zokunxiba okanye ukonakala; Ukongeza usebenzisa izixhobo zokuvavanya uxinzelelo ukuqinisekisa itywina elikhuselekileyo elithintela ukuvuza.

10. Qeqesha iqela lakho

Yenza uqeqesho olunzulu kuwo onke amalungu eqela lakho lelebhu ngendlela yokusingatha kwaye ligcine i-vials ngokufanelekileyo, igxininisa ukubaluleka kwengqibelelo ye-vial kunye nokulandela i-protokhols esekwe kuyo.

FUNDA INDAWO YOKUGQIBELA

Ungcoliseko lunokuvelisa iziphumo ezilahlekisayo. Soloko usebenzisa izixhobo ezicocekileyo, ukungazithandi iikhemikhali xa kudlulisa iisampulu kwii-vials. Ngokufanelekileyo, zilungiselele kwaye zisingatha iisampulu kwindawo ekhethiweyo ukuze kuncitshiswe umngcipheko wokungcola.

I-IPOPE-STPAN

I-Pre-Slit Septaziyilelwe ngokukodwa ukuba zinalentele inaliti ngaphandle kokwenzakalisa inaliti yesampulu, zinciphisa nawaphi na amathuba okuqhekeka xa kusetyenziswa i-autosamples xa usebenzisa i-autosamples xa usebenzisa i-autosamples xa usebenzisa i-autosmamples xa usebenzisa i-autosamples xa usebenzisa i-autosmamples xa usebenzisa i-autosamples xa kusetyenziswa intsholongwane.

Ukuqonda okubanzi kwe-PTFO / Isiliki septa, sukuphoswa kweli nqaku: "Ukuhlola iPremiyamu yePrefu kunye ne-Silicone Septa. "

13Main ilebhu echanekileyo

USepta unokunxiba ngokuhamba kwexesha kwaye ukhokele kwiingxaki ezinjengeesampula kunye nongcoliseko, ke kuyacetyiswa ukuba ubeke endaweni amaxesha ngamaxesha, nokuba ziyabonakala zingabonakali. Ukubuyiselwa rhoqo kusetyenziswa ukusetyenziswa kunye nokuba loluphi uhlobo lweesampulu ezihlalutyo.

Ivoramu

Xa ujongene neesampulu ezixabisekileyo okanye ezinqongopheleyo, kubalulekile ukunciphisa ivolumu yesampulu kwi-Vial nganye kangangoko kunokwenzeka. Ukunyanzelwa kunokubangela ukuvuza kwesampulu, ngelixa ukugcwaliswa kwesampulu kunokuvumela i-inverress yomoya kwi-vials-ukufumana i-aquilibrium sisitshixo sokuqinisekisa ingqibelelo kunye neziphumo ezichanekileyo.

15.Khetha ubungakanani be-vial efanelekileyo

Ukukhuthaza ivolumu yesampulu kunye nejeyingile, khetha ubungakanani be-vial ohlangabezana nazo zombini. Ukugqobhoza i-vials ezincinci kunokubangela ukusebenza kakubi okanye ungcoliseko ngelixa ubukhulu becala bunokubangela ukuchitheka okanye ukonakala kweSepta.

16.check Siringes

Rhoqo hlola imeko yeenaliti zesirinji esetyenziselwa ukufaka iisampulu, njengoko iinaliti ezonakalisiweyo okanye ezinxibileyo zinokukhokelela kwi-Septa kunye nomonakalo we-vial. Ukulungiswa kwenkqubo yakho yokuphelisa-kubandakanya ukutshintshwa kwenaliti rhoqo-kubalulekile ukukhusela ingqibelelo.

17MOtotsheli

Kwi-chromatography yegesi esebenzayo, qinisekisa ukuba uxinzelelo lwegesi loThengikazi luhlala lungangqinelani ngawo onke amaxesha. Ukuguquguquka kuxinzelelo kunokubangela ukuvuza kunye nomonakalo weSeptha; Ukukhusela ngokuchasene neengxaki, imigca yegesi kufuneka ihlale ikhuselekile ngaphandle kwemiba yokuvuza.

18.Ndibhengezo

Gcina iirekhodi ezineenkcukacha zokusetyenziswa kwe-vial kunye nolondolozo. Oku kubandakanya ukulandelela inani leendlela, uhlobo lweesampulu eziqokelelwe kunye nemeko ye-septa kunye ne-caps. Ngokwenza oku, ikuvumela ukuba uchonge iipateni kwangoko kwaye ubhekise kwimicimbi enokubakho ngokukhawuleza.

Nge-198, ukulungelelanisa izixhobo

Qiniseka ukuba uyahlulahlula izixhobo ze-chromatography ezifana ne-autosamples kunye neenkqubo zokuphelisa ngamaxesha athile ukuqinisekisa ukuphathwa kwesampulu efanelekileyo ngokunciphisa ukuthembeka kwengqondo okunokulalanisa kwimpazamo. Ukulinganiswa ngokuchanekileyo nako ukugcina ukusebenza kwezixhobo ngokugcina iinkqubo zokuphatha kwesampula.

I-20.PRRORMFOFFOFOM PER

Iinkqubo zokuLawulwa komgangatho Ukugcina ingqibelelo ye-phemfor ye-vial vision njengenxalenye yeenkqubo zakho zelebhu. Rhoqo hlolisisa isampulu ye-vials yokuvuza okanye ungcoliseko ukubamba imiba kwangoko kwaye uphephe ukuphindaphinda kwakhona.

Ukugcina ingqibelelo xa usenza uhlalutyo lweChromatography kubalulekile ukuba ufumane iziphumo ezichanekileyo nezinokuthenjwa. Ngokulandela ezi ngcebiso, unokuphucula kakhulu ukuthembeka kwenkqubo yakho yeChromatography, ukuqinisekisa idatha esemgangathweni yohlalutyo. Ngaphandle kwenqanaba lakho lobuchule kule ndawo, ezi zenzo ziya kukwenza ukuba uzivelise i-chromatography ye-chromatography ethembekileyo yovavanyo.

Ndinomdla malunga ne-vials ye-HPLC? Fumana iimpendulo ezigqibeleleyo kwinqaku lethu:I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Ungayigcina njani kwaye uqokelele i-verography efanelekileyo

Okulandelayo:

Izinto ezi-3 eziphambili ekufuneka ziqwalaselwe xa ukhetha i-vials ezantsi kwiisampulu zakho

Iimpawu: Ukuvavanywa kweziyobisi ulawulo lwemeko Inzululwazi Umgangatho we-Water Ukulungiswa kweekhemikhali Ukuthathwa kweziyobisi i-vial-sepga I-chromatography-vials Vials-chromatography-2ml I-HPLC-Vials-Ixabiso I-HPLC-I-VAL-2ML I-HLLC I-HPLC-vils-vials umzi-mveliso we-HPLC

Umbuzo