I-chromatography yindlela esebenzayo yohlalutyo eqashwe kwiilebhu zokwahlula kunye nokuhlalutya umxube onzima. I-vialsgraphy i-vials idlala indima ebalulekileyo kule nkqubo ngeesampulu zezindlu ukuba zihlalutywe; Ukugcina okufanelekileyo kunye nokuphatha iinkqubo zezi vils kubalulekile ukuqinisekisa iziphumo ezichanekileyo ukusuka kuhlalutyo lwakho. Esi sikhokelo sibanzi esibanzi siza kuthetha ngeyona ndlela iphambili yokuphathaI-chialsgraphy ye-chromatographykwiindidi ezahlukeneyo zesampulu.

Ufuna ukwazi iimpendulo ezingama-50 malunga nee-vials vials, nceda ujonge eli nqaku: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Kutheni ukugcinwa ngokufanelekileyo nokusingatha kubalulekile

I-vialsgraphy ye-chromatography iqhele ukwakhiwa kwiglasi, isetyenziselwa ukugcina iisampulu ezingamanzi eziya kuthi ziphinde zihlaziywe ngeendlela ezifana ne-chromatography (LC) okanye i-chromatography (GC). Bakho ngomoya ukunqanda ungcoliseko kunye nokuphuma kwamanzi okunokutshintsha iziphumo; Ngesi sizathu sodwa ukugcinwa ngendlela efanelekileyo kunye nokusingatha kubalulekile iziphumo ezichanekileyo. Nazi ukuba kutheni imicimbi efanelekileyo yokusombulula:

Ukugcina ingqibelelo yesampulu:Ukugcina iisampulu ezikwi-vils ezibonisa ukuba zikhuselekile zivela kungcoliseko, ukonakaliswa, okanye ukuncedisa - ukunceda ukuba bamele abamele imithombo yabo yokwenyama.

Ukuthintela iimpazamo zohlalutyo:Ingxolo engeniswe ngokuphathwa okungafanelekanga okanye ukugcinwa kunokwazisa ukutyhola kwiisampulu kwaye ziphumo kuhlalutyo olubi okanye ubuxoki lwendalo.

Ukhuseleko loKhuseleko:Ukubandakanya iingcali kubalulekile xa ubeka iisampulu ezinokuba yingozi okanye enetyhefu; Ukuphatha ngokuchanekileyo kunciphisa umngcipheko wokuveza kubahlalutyi.

1. Ukucoceka kubaluleke kakhulu
Ukugcina ucoceko xa ugcina i-vialsgraphy ye-vials, qinisekisa ukuba bakhululekile kwintsalela okanye intsalela ngaphambi kokubeka iisampulu ngaphakathi kubo. Hlambulula usebenzisa i-solvent efanelekileyo ngaphambi komisa ngokupheleleyo ukunqanda ungcoliseko lweesampulu zakho.

2. Khetha i-vials esemgangathweni ephezulu kunye ne-caps

Ukukhusela ukuthembeka kweesampulu zakho, tyala imali ekumgangatho ophezului-vials kunye ne-capsukusuka kubathengisi abathembekileyo. Izinketho zexabiso eliphantsi zinokubangela ukuvuza okubengezelayo; Iglasi yesifo seglasi idla ngokuxhathisa imichiza kwaye incinci ekusebenziseni kuneenketho zeplastiki.

I-3. Ilebheli i-vials ngokufanelekileyo
Kubalulekile ukuba i-vial nganye ibhalwe ngokucacileyo ngolwazi olufanelekileyo, kubandakanya negama layo negama kunye nayo nayiphi na iinkcukacha ezifanelekileyo ukunciphisa ukudideka kunye nokuqinisekisa ukulandelela. Ngokubhala ngokucacileyo i-Vials ngaloo ngcaciso unciphisa umngcipheko kunye nokuqinisekisa ukulandelela.

4. Gcina kwindawo elawulwayo

Ukuqhubeka nokuguquguquka kobushushu kunye nokuvezwa kukukhanya ubuncinci, kunye nokugcina iisampulu ezinokuthi zichaphazeleke bubushushu okanye ukukhanya kwexesha. Eli nyathelo liqhosha njengoko ezi zinto zinokubangela ingozi engalunganga kwiisampuli ezigcinwe apha.

5. Gcina i-vills fet

Gcina i-villa uthe tye ukuze uphephe ukuvuza okunokubakho ngelixa kwasanda kombutho kunye nokufikeleleka. Ukuzigcina sithe tye kukufumanisa ukuba sinemibutho kunye nokufikeleleka.

I-6. Gcina iLog Vial

Kububulumko ukugcina irekhodi layo yonke i-vial kwi-inventri yakho, ephawula imixholo yabo, iMihla yokuGcina ukuze kuqinisekiswe iisampulu zasetyenziswa ngexesha elifanelekileyo kunye nemicimbi yokugcina igqityiwe ngokukhawuleza.

Ufuna ukwazi ukuba ukhetha njani i-crmial vs vs vial vs. screw cap vial ?, Jonga eli nqaku:I-crmp vial vs i-vial vial vs.crew cap vial, njani? \/ Want to know how to different HPLC vials?

Eyona misebenzi iphambili yokuphatha i-vialsgraphy ye-vials

1. Qesha iindlela ezifanelekileyo

Ukunciphisa umngcipheko wongcoliseko xa uphatha i-vialsgraphy ye-vials, hlala ulandela iindlela ezifanelekileyo ukuze uzinciphise. Ngokukodwa, sebenzisa isithole esicocekileyo okanye imibhobho xa uhambisa iisampulu kwaye ayeke ukuchukumisa umphetho we-Vim ngeminwe yakho.

2. Sukugqitha

Sukuphumelela i-vialsgraphy ye-vials njengoko oku kunokubangela ukuqhekeka. Qiniseka ukuba kukho i-ntloko enkulu ekhoyo yokwandiswa kwe-thermal ukuba igcinwe kubushushu obahlukeneyo, njengokugqitha kunokubangela ukonakalisa i-vials kunye nokuqhawuka kwimixholo yayo.

3. Vavanya i-vials ngaphambi kokusetyenziswa

Ukuqinisekisa ukusetyenziswa ngokufanelekileyo kwe-Vial, hlola ukuba zenziwe ngamatye, iitshipsi, okanye nasiphi na isiphene ngaphambi kokwenza ukhetho lwakho. Ii-vials ezonakalisiweyo kufuneka zilahlwe ngokukhawuleza njengoko zinokukhokelela kwimiba yokuvuza kunye nemicimbi yokungcola.

I-4. Ukhuseleko lwe-vial vial vial ngokuchanekileyo

Ukugcina itywina lomoyaI-vial caps, Qinisekisa ukuba bayaqiniswa ngokukhuselekileyo besebenzisa i-threque yomsele okanye i-cap crimper yokutywina okungaguqukiyo nangokuchanekileyo.

I-5. Umda wokuvezwa komoya

Ukucutha ukonakaliswa kwesampulu ngenxa yokuvezwa komoya, ukunciphisa ukuba i-voials ivulekile ukuba ivulekile kangakanani ngaphambi kokubuyisa iisampulu kuzo. Oku kunceda ukuphepha ukuvezwa komoya okugqithisileyo ngexesha lokurhoxiswa kwabo.

Ukuqwalaselwa okhethekileyo kwiisampulu ezibuthathaka

Ukuba isampulu yakho inobuthathaka kakhulu, usebenzisa izisombululo zokugcina ubushushu ezinjengeefriji okanye i-freezers zokugcina ixesha elide kunokuba luncedo. Xa ilebhile i-vials ukubonisa amaqondo obushushu ogcino efunekayo ikwanceda.

Iisampulu ezibuthathakaXa usebenza kunye ne-contands ebuthathaka, sebenzisai-amber okanye i-vials enemibalaUkukhusela iisampulu kwifoto-kwaye uzigcine kwizitya ezimnyama okanye ze-opaque ukuze unciphise ukuvezwa kokukhanya.

Iisampulu ze-hygroscopic:Xa usebenza neesampulu ezinobuthathaka

Ukucoca i-vials kunye nokulungiselela i-vials, kunye neemvulophu (kunye okanye ngaphandle kwe-rinsing amanzi)

Iiarhente zokucoca:Xa ucoca i-Vials, iiarhente ezifanelekileyo ezinjengezinyibilikisi eziphezulu, iingcingo ze-acid okanye izisombululo ze-acid ngokuxhomekeka kuhlobo lwezinto ezingcolileyo kufuneka zisetyenziswe njengoko kufuneka ukucocwa. Emva koko, hlamba i-vial nganye ngononophelo ekupheliseni nayiphi na i-ntsali eshiyekileyo evela kwiiarhente zokucoca ezisetyenziswa kule nkqubo.

Iindlela ezomisayo:Ngaphambi kokubeka iisampulu zibe yi-victs, qiniseka ukuba yonke indawo yomile ngokupheleleyo njengoko isomile esingaphelelanga kunokubangela ungcoliseko kwaye ulalanisa ingqibelelo yesampulu.

I-Wan Ukufumana ulwazi olupheleleyo malunga nendlela yokucoca i-vialsgraphy yesampulu ye-chromatography, nceda ujonge eli nqaku:Iyasebenza kakuhle! Iindlela ezi-5 zokucoca iimodeli ze-chromatographys

Kulumkele ungcoliseko olunqamlezayo xa uphakamisa iisampulu

Ukwenzela ukuthintela ungcoliseko olunqamlezileyo xa uhambisa iisampulu eziphakathi kwezikhongozeli okanye i-vials, ezilahlekileyo okanye ezicocekileyo okanye ezomileyo ukuze zisebenzise iisampulu ezingenanto xa zisebenza.

Izixhobo ze-Vial:Xa usebenza neesampulu ezibuthathaka okanye ezinobungozi, usebenzisaIzixhobo ze-VialNjenge-tiezers okanye iigloves zokuphatha i-vials zinokukunceda ukunciphisa unxibelelwano ngqo phakathi kwezandla kunye neevils.

Iifilitha zesirinji:Xa uthatha iisampulu ezivela kwi-vils, usebenzisa iifilitha zesirinji ezinobukhulu obufanelekileyo bokususa iinkcukacha kwaye ziqinisekise ukuba ukucoceka kweesampulu zakho kunokunceda ekuqinisekiseni ukuthembeka kwabo kunye nempumelelo.

Nciphisa ukuvulwa kwe-vial:Kwiisampulu ezifuna ukufikelela rhoqo, iivils ezinkulu kufuneka zithathwe njengokunciphisa ukuba kufuneka zivulwe kangaphi kwaye zivaliwe.

Iirekhodi zokugcina kunye namaxwebhu

Ukufumana ingqibelelo efanelekileyo, gcina irekhodi le-elektroniki yemixholo ye-Vial, iimeko zokugcina kunye naluphi na utshintsho ekuhambeni kwexesha kwisampula. Olu xwebhu lwedijithali lusebenza njengomthombo obalulekileyo wokutshekisha.

Isoftware yesoftware yesampula:Iinkqubo zolawulo lwe-Lab zoLwazi (imilimi) okanye isoftware yokulandela umkhondo yindlela esebenzayo nefanelekileyo yokongamela iilebhu ezinkulu ezinomthwalo omkhulu, ngakumbi xa kulandelwa uluhlu lweempahla.

Uqeqesho kunye neMfundo

Imfundo yayo yabasebenzi: Kubalulekile ukuba abasebenzi baselunxwemeni bafumane uqeqesho olwaneleyo kwiinkqubo zokuphatha okukhuselekileyo kunye nokugcina. Uhlaziyo rhoqo ngokubhekisele kwiindlela ezilungileyo kakhulu unokuyekisa kakhulu ithuba leempazamo ezenzeka kwiindlela zabo zemihla ngemihla.

Ukumiliselwa kweeprotokholi zokhuseleko: Ukuseka iinkqubo zokhuseleko xa uphatha ngezinto eziyingozi, njengokubhala iibheyibheli ngokuchanekileyo kwaye uzigcine kwiindawo ezichongiweyo. Xa kufuneka, nxiba izixhobo zokhuselo (PPE).

Ukugcina okufanelekileyo kunye nokuphathaI-chialsgraphy ye-chromatographyIqhosha lokuxhasa ukuthembeka kwedatha kunye nokuvelisa iziphumo ezinokuthenjwa. Ukubambelela kwezona ndlela zilungileyo malunga nokugcinwa kwesampulu kunye nokukhusela kunokuqinisekisa ukuqinisekiswa kweziphumo zenkoso; Soloko ubhekisa kuzo naziphi na izikhokelo ezifanelekileyo ezibonelelwe yilebhu yakho okanye umvelisi wesixhobo sohlalutyo ukuze ziqwalaselwe kwi-Vial kunye neSampulu. Ngogcino olufanelekileyo kunye nokuphatha ngendlela ekhoyo, ukuqhuba uhlalutyo oluvelisa i-chromapografic enentsingiselo nechanekileyo kufuneka ilula kunangaphambili!

Fumanisa i-ralaticale emva kokhetho lwe-vialparent ye-chromatography kunye nendima yabo ebalulekileyo ekuqinisekiseni iziphumo ezichanekileyo.Kutheni le nto i-vill ecacileyo ekhethwe kwi-chromatography?

Ngaphambili:

Indlela yokuhlanganisa ngokufanelekileyo i-vialsgraphy ye-vials enesikhafu kunye ne-ptfe-silicone septa

Okulandelayo:

Iingcebiso ezingama-20 zokugcina ingqibelelo ngexesha lohlalutyo lweChromatography

Iimpawu: Ukulungiswa kweekhemikhali Umgangatho we-Water ulawulo lwemeko i-vials-rack I-HPLC-vial-rack I-chromatography-vials igesi-chromatography-vials I-HPLC-vils I-2ml-hplc-vials I-HPLC-I-VAL-2ML I-HLLC umzi-mveliso we-HPLC Umthengisi we-HPLC-Vials I-HPLC-vials-ye-leb-lab I-HPLC-vials-yentengiso

Umbuzo