Iindlela zeChromatography zibalulekile kwimihlaba eyahlukeneyo yesayensi, ukuqhuba uhlalutyo oluchanekileyo kunye nokwahlulahlula umxube ontsonkothileyo. Nangona kunjalo, phakathi kweenkqubo ezicekecekileyo kunye nokuqwalaselwa kweenkcukacha, isisongelo esibukhali kodwa siyinto ebalulekileyo esenziweyo ngaphakathi kwelebhusayithi:I-chromatography vialungcoliseko. Ezi zikhongozeli zibonakala zingenazimvo, zibalulekile kwiisampulu zezindlu, zichaphazeleka kuluhlu lweentlobo ezingcolisekileyo ezinokuthi zityeshele ukuthembeka kweziphumo zovavanyo. Kweli nqaku, sinengxaki yokufumana imagazini ye-chiromatography ye-chiromatography, ehlola imvelaphi yayo, i-ramicare yayo kwiziphumo zovavanyo, kunye nezicwangciso zokunciphisa.

I-1. Imithombo yongcoliseko lweChromatography

Ungcoliseko lwe-vialsgraphys lunokuvela kwimithombo emininzi yemithombo, ngamnye ubeka imiceli mngeni eyodwa yokuchaneka:

Ungcoliseko lwangaphandle:Imeko yelebhu, nangona kukho iinzame zokugcina ukungasebenzi, amazibuko omoya, iintsalela ezivela kuvavanyo lwangaphambili, kunye neearhente zentsholongwane. Olu hlazo lwangaphandle olungenantlonelo lwe-vials le-vials kwimijelo yomoya okanye umfowunelwa ngqo, ukwazisa ukungcola okungafunekiyo kwiisampuli.

Ungcoliseko lwangaphakathi:Kuyamangalisa ukuba, imithambo ngokwayo inokusebenza njengezithambiso zongcoliseko. Izinto zentsalela ezivela kusetyenziso lwaphambi kwexesha, i-leachates kwizixhobo ze-vial, okanye ukungafezeki kwe-microscopic kwiglasi kungazenzela ubunyulu beesampulu.

Ukupheliswa kwenkqubo:Impazamo yomntu ihlala igalelo elibalulekileyo kungcoliseko lweChromatography. Ukuphambuka kwiiprothokholi ezivela ngexesha lokuphatha isampulu, ukugcinwa, okanye ukulungiswa kwe-vial kungazi kakuhle ukungaziphathi kakuhle, ukujongela phantsi ukuthembeka kweziphumo zohlalutyo.

Phonononga izicelo ezahlukeneyo ezili-15 ze-vialsgraphy yeChromatography kweli nqaku. Hlalani kwizinto zabo zokusebenza kwaye ukubaluleka kwimimandla eyahlukeneyo yesayensi:I-15 izicelo ze-vialsgraphys kwimimandla eyahlukeneyo

2. Impembelelo ye-chromatography yongcoliseko kwiziphumo zovavanyo

Iziphumo zeI-chromatography vialUngcoliseko lukhulisa yonke inkqubo yohlalutyo, ukuphosa amathandabuzo malunga nokwamkelwa kweziphumo:

Iziphumo zovavanyo ezigqwethekileyo:Ungcoliseko, nokuba amakhemikhali, okwemvelo, okanye ngokwasemzimbeni, unokusebenzisana ngokuhlalutya ngeendlela ezingalindelekanga, ukutyhoboza iiprofayili ze-chromatographic kwaye ukhokele.

Ukuzala kwakhona:Ubukho beengcongconi ezizisa ukungafani nokungalindelekanga kwiinkqubo zovavanyo, ukubonelela ngeziphumo kunzima ukuphindaphinda. Ukungahambelani kokuphindaphinda ngokuphindaphindiweyo kukuthembeka ukuthembeka kwezigqibo ezivela kuhlalutyo lweChromatographic.

Isixhobo seSixhobo:Ngaphandle kweqhinga lokuhlalutya iziphumo, ungcoliseko lwe-vial lubeka umngcipheko obonakalayo kwi-chromatographic yesixhobo. Ukufakwa kwempahla ebunjiweyo kwizinto ezibuthathaka njengeekholamu okanye iikholamu zinokuphazamisa ukusebenza, kunyanzelisa ukulungiswa kweendleko okanye ukutshintshwa.

Fumanisa amanyathelo afanelekileyo okulungiselela i-veromatography ye-veromatography efanelekileyo, ukuqinisekisa ukuba iziphumo zohlalutyo ezinokuthenjwa. Inkosi i-Guert kwiindlela nje ezintandathu ezilula !:Amanyathelo ama-6 okulungiselela i-vialsgraphy ye-vials yohlalutyo

3. Uthintelo kunye neendlela zokulungisa

Ukunciphisa umngcipheko wokungcoliseka kweChromatography onyanzelekileyo ukuba indlela ebanzi equka iquka amanyathelo asebenzayo kunye nongenelelo lokuphendula:

Iiprotocoyine zeLebhu yeLebhu:Ukucocwa rhoqo kunye nokubulawa kwabantu kwindawo yeLebhu, izixhobo, kunye neenkqubo zomoya zokuphela komoya zinciphisa i-vollux ye-influx ye-chinanants yangaphandle, ukukhusela ukuthembeka kwe-vials vials kunye neesampulu.

Uvavanyo lwe-valine gesi kunye nolondolozo:Uvavanyo lwamaxesha ngamaxesha ococeko kunye nengqibelelo yenza ukuba kufunyaniswe kwangoko ukungcoliseka okanye ukonakala. Ukutshintshwa kwangoko okanye iinkqubo zokucoca ngokuchanekileyo ukuthobela umngcipheko wempazamo ezingenamsebenzi.

Ukubambelela kwiinkqubo ezimiselweyo:Iinkqubo zoqeqesho ezomeleleyo kunye nokubambelela kunyango lweeprothokholi ezimiselweyo kubalulekile ukunciphisa ungcoliseko olunetyala lomntu. Izikhokelo ezicacileyo zokuphatha isampulu, ukulungiselela i-vial, kunye nokugcinwa kokukhuthaza ukungqinelana nokuzalwa ngokutsha kuhlalutyo lweChromatographic.

UKUSETYENZISWA KWEENCWADANA ZOKUGQIBELA EZIQHELEKILEYO:Ukutyala imali kwi-vials ephezulu ye-chromatography eyenziwe kwizixhobo ze-ITRT kunciphisa amathuba okuba lemfundo okanye ukungcola kokubeka isampula ingqibelelo yesampulu.

Ukuqukumbela

I-chromatography vialUngcoliseko, nangona uhlala egubungela ezinye imiceli mngeni yovavanyo, ubeka isoyikiso esibalulekileyo ekuthembekeni nasekuthembekeni kweziphumo zohlalutyo. Ngokuqonda imithombo eyahlukeneyo yongcoliseko, ukuqonda iimpembelelo zayo kwiziphumo zovavanyo, kunye nokuphumeza amanyathelo okuthintela ezomeleleyo, iilebhu zinokuqinisa iinkqubo zazo zohlalutyo ngokuchasene nengozi engathandekiyo. Ngokubeka phambili ukugcinwa kwesampulu inyulu kunye nokugcina isixhobo, abaphandi baxhasa imigaqo-siseko yesiseko sophando lwenzululwazi, baqinisekisa ukuba i-chromatographic ichanile, iphinde ichanekile, kwaye inolwazi oluchanekileyo.

Vula iimpendulo kwimibuzo engama-50 eqhelekileyo malunga nee-vials ze-HPLC kwinqaku elipheleleyo. Fumana ukuqonda kwiindidi ze-vial, ukusetyenziswa kunye nokulungiswa: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Yintoni ikepusi enesifo?

Okulandelayo:

Ukujongana nokuphazamiseka kwangasemva kwi-vialsgraphy ye-chromatography