I-Chromatography, ubuchule beNdoda kwi-chemistry yohlalutyo, iququzelela ukwahluka kunye nokwahlulahlulahlulahlulahlulahlulahlulahlulwa ngaphakathi kwemixokelelwane. Ngaphandle kwesicelo esixhaphakileyo, ukuhlalutya kwe-chromatographic kukuphazamiseka kungenelelo lwangemva, nto leyo enokulalanisa ngokuchanileyo kunye neziphumo. Omnye umthombo obalulekileyo wokuphazamiseka kwabanga mva kwi-vialsgraphy yeChromatography, enokwazisa ungcoliseko kunye ne-arhentes kwi-matrix yesampula. Kweli nqaku, sihlala sinengxaki yokuphazamiseka kwemvelaphi evela kwiI-chialsgraphy ye-chromatography, hlolisisa izizathu ezahlukeneyo kwaye ucebisa ngeendlela zokunciphisa.

Ukuqonda uphazamiso lwangemva

I-Supersies I-Sport

I-vialsgraphy ye-chromatography inokugcina ungcoliseko lwentshukumo kwiinkqubo zemveliso okanye ukusetyenziswa kwangaphambili. Ezi zingcongconi zinokubandakanya uluhlu lwezinto, kubandakanya i-thabristants, ii-arhente zokukhuphela i-mold, okanye i-polymer yongezwa. Xa la manyanzazana adandle kwisampulu, angenisa ingxolo yesiseko kwaye aphazamise inyani kunye nokuqatshelwa kohlalutyi. Ubukho babalungu onjalo bunokukhokelela kwincopho yobuxoki, benciphile imiqondiso-kwingxolo, kwaye balalanisa kwi-chromatographic.

Ufuna ukwazi ngakumbi malunga nokusetyenziswa kwe-vialsgraphys, nceda ujonge eli nqaku:UKUJONGA ISICWANGCISO SOKUGQIBELA

Izahlulo kwaye ungene

Izixhobo ezithile ze-Vial, ngakumbi iiplasitiki, zinemveliso yokukhulula imilinganiselo ekhoyo xa ityhilwe kwii-slichents okanye iisampulu. Ezi zinto zikhutshiweyo zinokuvela kwi-vial matrix okanye i-songers ezisetyenzisiweyo kwi-fabritation yayo. Emva kokuba le nto isampulu, le mimandla inokungcolisa inkqubo yohlalutyo, ikhokelela kumanqanaba angasemva kunye nokuphazamiseka kunye nohlalutyo kwi-Peaks. Ubukho bokukhululeka bubeka umngeni obalulekileyo kuhlalutyo olubuthathaka, ngakumbi kumashishini okutya apho ukufunyanwa kwenqanaba le-tracemount.

I-adsorption engaphezulu

I-chromatography vialIindawo eziphezulu zinokubonakalisa iipropathi ezifihlakeleyo, apho zihlalutya okanye iisampulu zamanqaku ezilandela iindonga ze-vial. Le phenonon ibizwa ngokukodwa kwi-vials yeglasi, apho amaqela e-Silanol kumphezulu onokuqulunqwa ngePolar okanye ahlawulwayo. I-adsornge yomhlaba ikhokelela kwilahleko yesampulu, ixhaswe ngamaxesha, kwaye igqitywe amaxesha, ngaloo ndlela ishukumisa intsingiselo ye-chromatographic kwaye inciphise imvakalelo yohlaluko.

Izicwangciso zokunciphisa

Ukukhetha i-Vial

Ukukhetha i-vials eyakhiwe kwizixhobo ngoncedo oluncinci lwangemva kubalulekile ekunciphiseni izinto ze-chromatographic. I-Borossililicicate yeglasi, idume nge-interters yabo kunye neeprofayili eziphantsi, zizinto ezikhethiweyo kwizicelo ezininzi zohlalutyo. Ngenye indlela, i-vials idityaniswe nge-Intert Polymers, njengePolytetraflum (PTFE) okanye i-PTFE) okanye i-silicone, yenza ukuxhathisa okukhutshiweyo kukuphucula ukusebenza kwe-chromatographic.

Ukufuna ukwazi ukukhetha phakathi kwe-crms vials, i-snap vials, kunye ne-screw cap? Jonga eli nqaku lokuqonda ngokukhetha ukhetho olufanelekileyo lweemfuno zakho zeChromatography. Yijonge ngoku !:I-crmp vial vs i-vial vial vs.crew cap vial, njani?

Ukuphathwa kwangaphambili

Phambi kwesampulu, i-vials kufuneka iqhubeke ngokucokisekileyo ukuphelisa ukungabinamsebenzi kunye nokunciphisa uphazamiso lwangaphakathi. Le nkqubo ibandakanya i-vinkings yokuphononongwa ngeenyibilikisi ezifanelekileyo, ezinje nge-methanol okanye amanzi, ilandelwe ngokomiso phantsi kweemeko ze-intert. Ngokususa imithombo yongcoliseko, imeko yangaphambi iqinisekisa ukuzinza kwempawu zesiseko kwaye iphucule ukuzala kweMilinganiselo yeChromatographic.

Ulawulo lwemeko

Ukumiliselwa amanyathelo olawulo lomgangatho onamandla kubalulekile ekuqinisekiseni ukuthembeka kwe-vialsgraphy ye-vials kwaye kunciphisa uphazamiso lwangaphakathi. Iilebhu kufuneka ziqhube uphononongo lwendlela yokucoceka, ukukhangela iziphene ezibonakalayo, umcimbi wamanyathelo, okanye intsalela engaphezulu. I-Vials kufuneka igcinwe kwindawo ecocekileyo nelawulwayo yokuthintela ungcoliseko phakathi kokusetyenziswa, nangayiphi na i-vials efakwe ngokukhawuleza ukuba ilahlwe ukunqanda iimpazamo zohlalutyo.

Ukhathalelo lwesampula

Ukuziqhelanisa neendlela ezifanelekileyo zokuphatha kuyinto efanelekileyo ekuthinteleni ukuphazamiseka okunxulumene nokuvalwa kwe-chromatographic. Abahlalutyi kufuneka balumkise ukunciphisa ukusebenzisana komhlaba, ukunqanda ukugqobhoza i-vials kunye nokuqinisekisa ukuthintela ukuthunyelwa kwesampulu okanye ungcoliseko. Ukongeza, ukusebenzisa isampulu yokuphela kwesampulu okanye i-robrigigation kunokususa umbandela wemali kwaye unciphise umngcipheko wesampulu, ngaloo ndlela kuphucule ukuchaneka nokuchaneka kweziphumo.

Ngaba ujonge ukuphakama kwi-HLLC yesampulu ye-HPLC? Dive kweli nqaku elifundisayo lokuqonda kweengcali kunye neengcebiso ezifanelekileyo zokuphucula i-chromatography yakho yeChromatography. Fumanisa ngakumbi ngoku !:I-HLLC yesampulu yokulungiselela ukulungiselela iziphumo ezilungileyo

Indlela yokuphumelela

Ukwenza i-chromatographic parameter idlala indima ebaluleke kakhulu ekunciphiseni ukuphazamiseka kwangaphakathi kunye nokuphucula intsebenzo yohlalutyo. Abahlalutyi kufuneka bavavanye ngokucwangcisiweyo iimeko, kubandakanya ukwakhiwa kwenqanaba leselula, ikhompyutha yekhompyutha, kunye nokusebenza kweeparamitha, ukwandisa impumelelo yokwahlulahlula kunye nengxolo yokuthula. Iimeko ezihle zohlobo oluhle zinokunciphisa iziphumo zengxolo yangemva, ziphucule isisombululo se-Peak, kunye nokomeleza ukuthembeka kwamanyathelo obuninzi.

Ukuqukumbela

Ukuphazamiseka kwangasemva okuphakathiI-chialsgraphy ye-chromatographyIbeka umngeni onamandla wokufumana iziphumo ezichanekileyo nezinokuthenjwa. Ngokuqonda ngokuqonda ngemithombo yongenelelo kunye nokuphumeza ngeendlela zokunciphisa izinto, iilebhu zinokunciphisa ifuthe lezinto ezinxulumene ne-vial kwi-vicromatographic. Ngokuzikhethela kwe-vila ye-vila, amanqanaba olawulo olusemgangathweni, kunye nendlela yokuphucula imvakalelo, ukuqina, kunye nokuzala kweMilinganiselo ye-CHRomatographic, ngaloo ndlela ufihlayo kwimilinganiselo ye-chromatographic, ngaloo ndlela uqhubekisa uphando lwenzululwazi, ukuthobela imigaqo, kunye nokusebenza kwemizi-mveliso kunye nokusetyenziswa kwemveliso.

Vula iimpendulo eziphezulu ezingama-50 malunga nee-vials ze-HPLC kwinqaku elipheleleyo. Fumana ulwazi lwengcali kunye nolwazi olufanelekileyo ukuphucula ulwazi lwakho lwe-chromatography. Jonga ngoku !: I-50 kakhulu imibuzo ebuzwa rhoqo kwi-vials ye-HPPL

Ngaphambili:

Ungcoliseko lweChromatography Gual: Ingozi efihlakeleyo kwilabhoratri

Okulandelayo:

Ukujongana nemiba engahambelaniyo phakathi kweendaba zeFiltha kunye neeFusi zeSampuls kwi-Fielth Fill

Ukujongana nokuphazamiseka kwangasemva kwi-vialsgraphy ye-chromatography

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