Uyini umehluko phakathi kwe-GC-MS ne-GC-MS \ ms?

Igesi i-Chromatography-Mass Spectrometry (GC-MS) kanye negesi i-Chromatography-Tandem Mass Spectrometry (GC-MS Spectrometry (GC-MS \ ms) amasu wokuhlaziya asetshenziswa emikhakheni esetshenziswa emikhakheni efana nezemithi, isayensi yezemvelo, ukuphepha kokudla, kanye ne-forensics. Womabili la masu ahlanganisa ukuhlukaniswa kwe-chromatographic ngokutholwa kwe-mass spectrometric kepha ahluke kakhulu ekusebenzeni, ukuzwela, ukucaciswa, kanye nezinhlelo zokusebenza.

1. Yini i-GC-MS?

I-GC-MS Couples Gas Chromatography, ehlukanisa amakhompiyutha aguqukayo ngamaphoyinti abilayo kanye nokuxhumana nesigaba semikhumbi, enesilinganiso esiningi, esikhomba amakhompiyutha asuselwa ku-Maste-To-Chwangukhweni (M \ z)

Izakhi ezibalulekile:

• Igesi Chromatograph: Ihlukanisa amakhompiyutha nge-Returnuon Time.

• I-Mass Spectrometer: Amakhompiyutha e-Ionizes (evame nge-Electron Ionization, eI) futhi akhiqize i-spectra
  

Ukulungiswa kwesampula:

Kufaka phakathi ukugcotshwa, ukukhishwa kwesigaba esiqinile (i-SPE) noma ukukhishwa okuwuketshezi (i-Lle), kanye nokutholwa kokuvela ekwenzeni amakhompiyutha GC-Ready.

2. Ithini i-GC-MS \ ms?

I-GC-MS \ MS yethula isigaba sesibili sokuhlaziywa kwabantu abaningi. Ngemuva kokukhethwa kokuqala kwe-Ion ku-quadrupole yokuqala (Q1), i-ion ekhethiwe eqokiwe Ama-Ions e-Ions abuye ahlaziywa yi-quadrupole yesibili (Q2). Ezinye izinhlelo zingafaka i-quadrupole yesithathu noma i-tof analyzer

Ukusebenza Okuthuthukisiwe:

• Ukucaciswa okuphezulu nokuzwela, ikakhulukazi ngokuqapha okuningi kokuphendula (MRM)

• Esebenza ngempumelelo kumatikuletsheni eziyinkimbinkimbi, ukuhlinzeka ngokuhlonza okuqondile lapho kungavela khona i-GC-STEP LINGE-GC-MS
  
  

3. Izicelo

GC-MS:

Esetshenziselwa kakhulu:

• Ukuhlaziywa kwe-VOC ejwayelekile

• UPHEPHA LOKUQHAWULA

• Ikhwalithi yokudla nobuqiniso

• Ukuhlolwa Okungcolisayo Kwezemvelo

Gc-ms \ ms:

Ilungele:

• Landelela Ukuqanjwa (isb., Ama-Pesticides emazingeni e-PPT)

• Ama-matric ayinkimbinkimbi lapho kwenzeka khona ukuhlangana.

• Ukuhlola okuphezulu kokubhekelwa ngokunambitheka usebenzisa i-MRM.

• Izicelo ezithuthukile, njengokudla kokudla nokutholwa kwe-Environmental Trace.
  
  

  

4. Umehluko obalulekile

I-5. I-FAQ

Q1: Yimuphi umehluko omkhulu phakathi kwe-GC-MS ne-GC-MS \ ms?
I-A1: I-GC-MS \ MS inikeza ukucaciswa okuthuthukisiwe nokuzwela ngokuhlunga okuningi kwesisindo kanye nezigaba ezengeziwe zokuqhekeza, ezilungele amasampula ayinkimbinkimbi nokutholwa kokulandelela

Q2: Kukhethwa nini i-GC-MS?
I-A2: Ukuhlaziywa okujwayelekile kwezinto eziguquguqukayo ze-organic lapho izidingo zokuzwela zilinganiselwe futhi zimatikuletsheni zilula.

Q3: Kufanele ngisebenzise nini i-GC-MS \ ms?
I-A3: Ngokuqanjwa kwe-Ultra-Traceation kumasampula ayinkimbinkimbi, ukuqapha imvelo, ukuhlolwa okuthe xaxa, kanye nokuhlonza ama-forensic.

Q4: Ngabe i-GC-MS ibhekele amakhompiyutha angaguquki?
I-A4: Hhayi ngqo; Ukususwa kuyadingeka. Okwezinhlanganisela ezinkulu noma ezishisayo ze-abiale, i-LC-MS noma i-LC-MS \ MS ingahle ilunge ngokwengeziwe.

Q5: Izindleko ziqhathaniswa kanjani?
I-A5: Amathuluzi we-GC-MS ngokuvamile abiza kakhulu (amashumi ezinkulungwane Usd). I-GC-MS \ MS Systems (nge-QQQ noma i-Ion Trap Configurations) ibiza kakhulu kakhulu kepha inikezela ukusebenza okuphezulu kokuhlaziywa okukhethekile

I-GC-MS kanye ne-GC-MS \ ms bobabili banikela ngamakhono okuhlaziya okuqinile. I-GC-MS \ ms ithuthukisa ukuzwela, ukukhethwa, kanye nokuqonda okuhle kakhulu - ikakhulukazi okuwusizo ekulandeleleni izinga, okuhlaziya okuphezulu okuhlaziyiwe. Ukukhetha kufanele kususelwe ezintweni ezifana nokuhlaziywa kwelitshe, ubunzima obuyinkimbinkimbi, imikhawulo yokutholwa, izidingo zokusebenzisa, kanye nesabelomali.

Bheka umhlahlandlela opheleleUyini umehluko phakathi kwe-GC-MS ne-GC-MS \ ms?

1. I-Chromatography. Ukundiza okuphezulu ngokuzwela nokukhetha: GC-MS ku-GC-MS \ ms (chromatography.com)

2. Isayensi ye-Wiley analytical. Ukuqhathaniswa kokuzwela kwe-GC-MS nge-EI ebandayo (I-AplyticalsciinceJours.onlinelibrary.Wiley.com)

3. Ama-Agililent GC-MS FAQs (I-Agilent.com)

4. Wiley. Tandem Mass Spectrometry Okuxhasiwe (En.wikipedia.org)

5. NCBI PMC. I-GC-MS \ MS PATESTHETIFE PLAYANI KWAMAKHAYA AMOMSPRERIC (pmc.ncbi.nlm.nih.gov)