Ungayikhetha kanjani phakathi kwe-HPLC ne-GC Vials? Ukuqhathaniswa kokujula kwezinto zokwenziwa kanye nokuhambisana

Ukuqalisa

Ekuhlaziyweni kwe-chromatographic, ukukhetha isampula elifanele le-vial elinomthelela ngqo ngokunemba kanye nokuvela kabusha kwemiphumela yakho. Le ndatshana ihlinzeka ngokuqhekeka okuphelele komehluko phakathi kwe-HPLC ne-GC Sampula Vers, ukugxila kwizimo zohlelo lokusebenza, izici ezibonakalayo, izindlela zokubopha, nokukusiza ukukhethwa kwelebhu.

I. Umehluko obalulekile phakathi kwe-HPLC kanye ne-GC Vials

1. Izimo zesicelo

HPLC VIALS: Ngokuyinhloko esetshenziswa ekusebenzeni okuphezulu kwe-chromatography ye-chromatography (HPLC) kanye nokuhlaziywa kwe-LC-MS, kufanelekile ku-non-flate, armilly, noma amasampula acwebezelayo.

Ama-GC vials: Isetshenziswa ku-chromatography yegesi ye-chromatography (GC) ne-GC-MS, edinga ukumelana okuphezulu (imvamisa> 300 ° C) nokuhambisana nama-solvents aguquguqukayo.

2. Izici Zokuklama Nezinto Zokuklama

Izinhlobo ze-Vial Feat:

I-HPLC:I-Screw Screw tops noma izihloko ze-Snap, ilungele ukufinyelela okuphindaphindwayo nama-auto-salplers.

GC:Imvamisa i-crimp top vers enezigqoko ze-aluminium ne-ptfe \ airtone septa yokubekwa uphawu okuphezulu.

Ufuna ukufunda ngezindlela ezi-5 zokuhlanza i-chromatography isampula ama-vials? Chofoza lapha ukuthola umhlahlandlela onemininingwane

Ukukhetha kwezinto ezibonakalayo:

I-HPLC:Ngokuvamile kwenziwa ngengilazi ebumsulwa ephezulu, ngezinguqulo ze-ander zamasampula abucayi.
GC:Dinga ukumelana okushisa okuphezulu-ingilazi ye-borosilucate kuvamile, ngepulasitiki ye-PFA esetshenziswe kuzinhlelo zokusebenza ezikhethekile.

II. Ukucatshangelwa okusemqoka kwezinto zokwakha ze-chromatography

1. Izinto ze-Glass

Ingilazi ye-BOROSILiLE: I-inert kakhulu nokuvimbela ukushisa; Ilungele ukuhlaziywa kwe-HPLC kanye ne-GC.
Ingilazi ye-amber: Inikeza ukuvikela okukhanyayo kwamasampula athambile akhanyayo.

2. Izinto zepulasitiki

I-Polypropylene (PP): Ibiza kakhulu futhi ilungele ukusetshenziswa okushisa okuphansi; i-solvent ekhawulelwe nokumelana nokushisa.
I-PumpluoroalOxy (PFA): Ukumelana okushisayo kanye nokuvuselelwa kwamakhemikhali; Ilungele ukulandela umkhondo nokuhlaziywa okushisa kakhulu kwe-GC.

Funa ukuhlolayini i-gc headpace? Chofoza lapha ukuze ubukeke ngokujulile

3. I-CAP ne-Septa izinto

Ptfe \ abicone septa: Vimbela ukungena ngaphakathi kwe-solvent ku-HPLC; Gcina ukwakheka ngaphansi kokushisa ku-GC.
Izindlela zokubopha uphawu: I-GC crimp caps idinga amathuluzi akhethekile, ngenkathi ama-hplc screw caps kulula ukuyiphatha.

III. Izindaba zokuhambisana nokuthi ungazigwema kanjani

1. Ukuguquguquka kwensimbi

Ukuvumelana |: Qinisekisa ukuphakama kwe-vial kanye nobubanzi bomdlalo we-trey (E.G., 2 ML Vavials).
Ukumelana nokubhoboza kweSepta: Ukuze uthole imijovo ejwayelekile ku-HPLC, khetha i-Septe nge-Elasticity ephezulu (E.G., PTFE \ Illicone Composite).

Ukubhekana nama-vial CAPS ne-Septa, kufanele wazi Cheya lapha

2. Ukulawulwa kwengozi yokungcolisa

Ukugwema ukukhishwa: Nge-GC ephezulu kakhulu ye-GC, khetha ingilazi ye-inert noma ama-pfa vials.
Ukuvimbela ukungcoliswa: Sebenzisa ama-vials aqinisekisiwe aqinisekisiwe (e.g., i-USP Class a).

IV. Ukuthenga Umhlahlandlela: Ukukhetha i-vial efanele ye-HPLC ne-GC

Chaza izidingo zokuhlola: Cabanga ibanga lokushisa, ukuguquguquka kwesampula \ ukuzwela okukhanyayo, kanye nemvamisa yomjovo.
Qondanisa izinsimbi zezinsimbi: Qinisekisa uhlobo lwentamo olufanele, ivolumu, kanye nokuhambisana kwe-autosampler.

Namanje awuqinisekiI-Crimp Vial vs Snap Vs Screen Screw Cap VIAL, Indlela Yokukhetha? Kahle logic ukukhetha lapha

Bheka amazinga ezimboni: Sebenzisa ama-vials nge-USP noma isitifiketi se-ISO sokuvumelana kwekhwalithi.
Ukuhlola Inzuzo Yezindleko: Ukuze uthole izivivinyo ezijwayelekile, khetha ama-vials ezingilazi ezingabizi; Ngokuhlaziywa kwe-TRACE, khetha izinto ze-inert.

V. FAQ - Imibuzo Ejwayelekile Iyaphendulwa

Q: Ngabe ama-HPLC asetshenziselwa ukuhlaziya i-GC?
A: Uma yenziwe ngengilazi ye-borossilte ne-heastrantraute (e.g., kuze kufike ku-400 ° C), ingahle isetshenziswe okwesikhashana. Kodwa-ke, ukusetshenziswa isikhathi eside ngaphansi kwamazinga okushisa aphezulu akunconywa.

Q: Ungakugwema kanjani ukungcoliswa kwe-vial?
A: Khetha ama-vials aqinisekiswe noma ahlanze ngokuzihlanza usebenzisa izixazululo ze-Chromatographic-grade.

Q: Ngabe ama-vials aphezulu e-crimp-top adingekayo ku-GC?
A: Ama-crimp vials anikeza ukubekwa uphawu okungcono emazingeni okushisa aphezulu, yize amanye amathuluzi asekelweni asekelwe ku-screw nama-adaptha.

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Ukugcina

Ukukhetha i-HPLC efanele ye-HPLC noma i-GC isampula yesampula kudinga ukucatshangelwa kwezimo zokuhlola, ukuhambisana kwensimbi, kanye nezimpawu zesampula. Ngokwandisa izinto zokwenziwa kwe-vial kanye nokucaciswa, ungathuthukisa kakhulu ukuthembeka kwedatha futhi unciphise izindleko zokuhlola eziphelele.