Kukonzekera kwa HPLC zitsanzo, zosefera syringe zimatenga gawo lovuta. Tinthu tating'onoting'ono tomwe timakondana ndi zipolopolo, zomwe zimafupika, moyo wofupika, zomwe zidathetsa zizindikiro, komanso ziletsebereka. Kusanthula komwe kumachotsa zosayerazi, kukonza zobwerezabwereza komanso kukhazikika kwa Baseline. Zosefera za singano-mawonekedwe zimapereka yankho losavuta, ndikuwonetsetsa kuti zitsanzo zisayeretsedwe pamaso pa jekeseni, kuteteza mzati wonsewo ndi chida, ndikulimbika kudalirika
Makhalidwe Ofunika a Zida Zosiyanasiyana za FUPRAne
PTF (polytetrafluoro sollene) membrane
Mankhwala Ogwirizana:Mwachilendo indr komanso yolimbana ndi ma solfornt onse oyipitsedwa, ma acid, ndi zitsulo, koma hydrophobic-sioyenera kufesa mwachindunji. Zoyenera kusinthika kwambiri (mwachitsanzo, acetonitrile, DMSO), kutentha kwambiri, komanso malo okhala. Amatulutsa pafupifupi popanda zowonjezera.
Mtengo Woyenda:Kapangidwe kaya kumafunikira kunyowa kokha ndi zosungunulira, zomwe zimapangitsa kuchepa pang'ono.
Mapuloteni amangiriza: Maonekedwe a Hydrophobic amatulutsa ma protein pang'ono osadziwika, ndikupanga zabwino kuti zisanthule.Gwiritsani ntchito milandu & zoperewera:Zabwino kwambiri pazinthu zamphamvu kapena zowoneka bwino kwambiri. Osati madzi osanyowa chisanachitike; Pewani kuyanika kupukuta kuti mupewe misozi.
Newn membrane
Mankhwala Ogwirizana:Hydrophilic; yogwirizana ndi madzi ndi okhazikika okhazikika. Ma sopo wamba ngati acetonitrile ndi DMSO amathandizidwa mokwanira, pomwe thf komanso fanoli wambiri amasamala. Kukhazikika kumachepetsa mu acid acid \ / zoyambira.Mtengo Woyenda: Mafuta othamanga mwachangu komanso osefera kuposa PTF, koma nthawi zambiri amachepetsa pvdf.Mapuloteni amangiriza:Khalidwe lalikulu losagwirizana ndi zitsanzo zolemera zolemera zomwe zimafuna kuchira. Gwiritsani ntchito milandu & zoperewera: Mosiyanasiyana chifukwa cha zosungunulira zachilengedwe zosungunulira kapena zitsanzo zozikidwa m'matumbo (E.g., acetonitrile, methanol). Kukhazikika ndi kusinthidwa, koma pewani mapuloteni kapena acid-acid zitsanzo za acid \ /
PVDF (Polyvinylidene fluoride) membrane
Mankhwala Ogwirizana:Hydrophilic; khola ndi madzi, mowa, ndi ma ketoni osalowerera ndale mpaka acidic \ / nyengo zoyambira. Kutumiza kotsika kwambiri kwa UV kuchepetsa nkhawa zakumbuyo.Mtengo Woyenda: Mphamvu kwambiri mosavuta, zopereka mwachangu kwambiri kuposa PTF kumbali yomweyoMapuloteni amangiriza:Zabwino kwambiri zitsanzo zokhala ndi zitsanzo, mapuloteni, ndi ma cell azikhalidwe.
Gwiritsani ntchito milandu & zoperewera:Zabwino kwa mapuloteni \ / peptide kapena mowa woledzera. Chidwi ndi zitsulo zolimba, zomwe zimatha kunyoza nembanemba.
PC (Polycarbonate) membrane
Mankhwala Ogwirizana: Malo osalala, ogwirizana ogwirizana ndi ma asidi ambiri, mchere, mafuta, komanso ma hydrocarbons. Osagonjetsedwa ndi methanol, makhomeni, ake, kapena aromatic.Mtengo Woyenda:Kugawa kolumikizana kwambiri kwambiri kumapereka zotumphukira kwambiri ndikuyenda mwachangu.Mapuloteni amangiriza:Malo otsika-agalasi amalepheretsa adsorption a mapuloteni ndi mamolekyulu akulu.
Gwiritsani ntchito milandu & zoperewera:Kuwerenga kwa tinthu tinthu tating'onoting'ono, tinthu tating'onotic tinthu tating'onoting'ono, ndi maphunziro am'mandende ofunikira kusungidwa kwathunthu. Kawirikawiri kawirikawiri mwakale.
Kuyerekeza magwiridwe antchito
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Mzere wapakati
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Mankhwala ogwirizana
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Kutentha
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Mapuloteni akumanga
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Ntchito ndi malire
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Ptche
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Kugonjetsedwa ndi mitundu yonse yamphamvu; hydrophobic
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Pansi
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Otsika kwambiri
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Magawo olimba anyama; Osati kuwonongeka popanda kunyowa
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Nylon
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Hydrophilic; Zabwino ndi Acn, DMSO; Chenjezo ndi Thf, Meoh
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Wasaizi
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M'mwamba
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Zochita zodzikongoletsera \ / shifker; Pewani mapuloteni & acid olimba
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Pvdf
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Hydrophilic; khola ndi madzi, mowa, matomoni
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M'mwamba
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Pansi
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Zitsanzo zazomwe zimachitika; Pewani Zida Zamphamvu
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Pc
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Yosalala; Zabwino ndi acid, mchere, mafuta; osati za Meoh, Ctones
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Wammwamba kwambiri
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Otsika kwambiri
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Tinthu tort \ / microscopy; Osati njira zachilengedwe hplc hplc
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Kusankha membrane yoyenera pa chitsanzo chanu
Mavuto Olimba kapena Okhala Ndi Matumbo Akuluakulu + Gwilitsa nchitoPtchechifukwa chophwanya malamulo ndi kuwonongeka kochepa.
Zitsanzo zazomwezi (zogulitsa) + zakumwa \ / madzi: Gwilitsa nchitoPvdfkwa mapuloteni ochepa omanga ndi kugwirizana kwakukulu.
Zochitika Zakale Zosungunuka:Gwilitsa nchitoNylonchifukwa cholingana komanso kulimba mtima - pewani mapuloteni kapena pH.
Kuwerengera kwa tinthu \ / microscopy: Gwilitsa nchitoPckukula kogwirizana ndi kusasamala.
Zindikirani:Nthawi zonse muziganizira zamankhwala zamankhwala, mtengo, ndi zofunikira posankha zomaliza.
Airiren amapereka zoseweretsa zapamwamba kwambiri ndi PTF, NYN, PC nembanemba kuti mukwaniritse zosowa zanu zonse za HPLC. Lumikizanani nafe pa zitsanzo kapena luso laukadaulo, ndipo tiyeni tikuthandizeni kusankha njira yabwino kwambiri.
Achizungu
Zauchina
