Mokhoa oa chrotogram, mokhoa o sebelisitsoeng ka mokhoa o fapaneng oa tlhahlobo ea mahlale, o sebetsa haholo boleng le botsitso bo boholo ba likarolo tsa oona, ho kenyeletsoa le vavers. Li-vielis, hangata li entsoe ka khalase kapa mefuta e fapaneng ea lipolanete, sebeletsa e le lijana tsa disampole le li-soelings ho li-chromatographic. Leha ho le joalo, khetho ea boitsebiso ba terista haholo e ka ama haholo ho nepahala le liphetho tsa chromatographic ka lebaka la mathata a lumellanang, solvent kapa lihlahla.

Li-vies tsa khalaseKe khale ke khetho ea setso bakeng sa li-chromatography tse ntle ka lebaka la ho hanyetsa ha bona khahlanong le lik'hemik'hale tse ntle tsa lik'hemik'hale tse ntle tsa lik'hemik'hale. Li loketse mefuta e mengata ea mehlala le li-solupole, ho kopanyelletsa le li-solvents le metsoako e sa tloaelehang. Leha ho le joalo, li-viels tse sa sebetseng tsa khalase li ka se be hantle bakeng sa lits'ebetso tse itseng moo samm ea mohlala kapa solvent li ka arabela ka lihlahla tsa khalase, tse isang litšila kapa litlolo tsa sekaseka.

Bakeng sa leseli le felletseng ka litlolo tsa HPLC Vial, oela ho se sehloohong sena se rutang bakeng sa tlhaiso-leseling e qaqileng ea litsebi tsa litsebi:Li-Ilial tsa Hplc Via:Ho ntlafatsa bonnete le botshepehi

Li-vials tsa polasetiki, ka lehlakoreng le leng, fana ka melemo e kang ho fetoha le maemo, ho lebetsana le ho fokola kotsing. Polypropingne (PP), polyethylene (Pepthylpenylpenten e tloaelehileng Lisebelisoa tsena ka kakaretso ha li lumellane le li-solvents tse ngata le li-acid tse ngata, li etsa hore li lokeloe ke mefuta e mengata e pharaletseng ea lits'ebetso. Leha ho le joalo, li-vaels tsa polasetiki li ka bonts'a mathata a lumellang ka li-solvent kapa sampole, a lebisang ho paterone ea litšila kapa ho thehoa.

E 'ngoe ea lintlha tse sa utloahaleng tse amang taelo ke lik'hemik'hale tsa lik'hemik'hale tsa sampole' me u lebelle. Mohlala, acidic, disampole tsa mantlha li ka arabela ka mefuta e meng ea khalase, e lebisang ho pente ea liphiri tsa tšepe kapa ho fetoloa ha PH. Ka mokhoa o ts'oanang, solvent ea manyolo e nang le polasetso e phahameng e ka baka ho ruruha kapa ho nyenyefatsa thepa e itseng ea polasetiki, e amang botšepehi ba batho ba boima ebile e ka silafatsa sampole.

Ho nahanela e 'ngoe ke lintho tse ling tsa bokaholimo ba lintho tse bonahalang. Li-vials tsa khalase hangata li na le sebaka se boreleli, se nang le hydrophilic, se fokotsang ho ba hlahlojoa le ho nolofalletsa litholoana tsa ho tsoala. Ho fapana le hoo, li-vaels tsa polasetiki li kanna tsa ba le sebaka sa hydrophobic se ka bang le ho feta kapa se nang le litlatsetso tse ka bang lihlahlobo, tse etsang tahlehelo ea mohlala ebile li fokotsehile.

Lekola sengoloa sena bakeng sa ho sheba likopo tse 15 tsa likhathatso tsa ciaromagraphy, li fana ka leseli le felletseng le mehlala e sebetsang:Likopo tse 15 tsa likhama tsa chromatography masimong a fapaneng

Ho sebetsana le litaba tse lumellanang, bahlahisi ba fana ka mefuta e mengata ea likokoana-hloko kapa mekhoa e etselitsoeng ho ntlafatsa likhang tsa lik'hemik'hale le ho fokotsa ho sebelisana le li-solling. Mohlala, silanization ea mekhabiso ea khalase e ka fokotsa ho bolela metsoako ea polar, ha metsoako ea polasetiki e sebelisoa ho li-vaels tsa polasetiki le fokotsa lesira la litšila.

Leha a tsoetse pele, ho bohlokoa ho li-croromaticgraphgraph ka hloko ho khetha lisebelisoa tsa vaela ka hloko ho latela litlhoko tse ikhethileng tsa mokhoa oa bona oa tlhahlobo le mohlala. Ho hlahlojoa ha tlhahlobo ho lokela ho etsoa ho lekola hore litsobotsi tsa litheko tsa kopo e itseng le ho netefatsa ho nepahala le liphetho tsa li-chroromagraphic.

Qetellong, litaba tse lumellanang kahare hoMalinyane a chrotographyLisebelisoa li ka ama haholo bohlokoa ho tšepahala le ho nepahala ha litholoana. Ho utloisisa thepa ea lik'hemik'hale tsa thepa ea vae le litšebelisano tsa bona tsa depolelle le li-solvel li bohlokoa bakeng sa ho khetha likhama tse nepahetseng le ho ntlafatsa tšebetso ea li-chromatographic. Ka ho bua ka lintho tse mabapi le kahlolo e mabapi le ho buisana le ho sebelisa lisebelisoa tse loketseng, li-chromatigraph li khona ho fihlella lihlahlobo tse tšepahalang le tse hlahisoang lipatlisisong tsa bona tsa lipatlisiso le likopo tsa bona.

Fumana mabaka a etsang hore ho hlahlobisise manyalo a khokhopomo a hokhukhunya a ka ntle ho khaba ea polasetiki ka ntle ho balekane bana ba mahlomoleng. Lekola melemo bakeng sa Botšepehi bo phahameng:Mabaka a 3 a holimo ho 3

E fetileng:

Mokhoa oa ho khetha mofuta o nepahetseng oa ho koaloa bakeng sa li-chromatograph tsa hau tsa chromatography

E 'ngoe:

Ho sebetsana le li-block tse bakoang ke lihlahisoa tsa li-confotogragraz tsa chromatography