Lithako tsa ChromatograftKe sesebelisoa sa bohlokoa sa ho boloka le ho sekaseka disampole ho laboratoring ea tlhahlobo ea maikutlo. Leha ho le joalo, e 'ngoe ea mathata a tloaelehileng hangata bafuputsi ba thulana le hore na li-block tse bakoang ke ntho e teng mohlapeng. Likarolo tsena li ka hlaha mehloling e fapaneng, ho kenyelletsa ts'ebetso ea sampole, ho tloha tikoloho le litaba tsa tikoloho esita le litsietsi. Ho utloisisa lisosa le tharollo ea li-block tse ngata ho bohlokoa ho boloka botšepehi ba li-chromatographgram.

Sebaka sa ts'ebetso ea mohlala ka mohlala o ka baka li-blockges tse tloaelehileng tsa chromatographic li ka ama ho nepahala le ho tlisa tlhahiso ea sephetho. Likarolo tsena li khona ho kena-kenana le kamano, etsa hore khatello e matla ea chrope e ka har'a chromatographic tsamaisong ea chromatographic e ka senya likarolo tse ling tsa liletsa. Ka hona ho bohlokoa ho sebetsana le bothata bona ka mokhoa o hlakileng.

Ho na le maano a 7 a ho thibela le ho fokotsa likamoko tse bakoang ke likarolo tsa likhatiso tsa ceromatography.

Sampution ea Mohlala:

Sampole ea mohlala ke bohato ba bohlokoa ho thibela li-block tsa likhama tsa ciaromatography. Pele ho ente tsamaiso ea li-chrotographic, mohlala o fetisoa ka sefahlehong ho tlosa boemo ba lihlahisoa. Lifilimi tsa syringe li sebelisoa hangata bakeng sa morero ona, 'me li fumaneha ka boholo ba pore tse fapaneng ho amohela mefuta e fapaneng ea mohlala. Memlone e fillil e kang nylon kapa PTFE e boetse e sebetsa hantle bakeng sa litlhoko tse ntle tsa ho feto-fofane. Ho khetha sethala se nepahetseng sa sefako se tiisa hore likaroloana tse kaholimo ho phallo e khethehileng li bolokiloe, li bolokiloe kotsi ea moferefere oa val.

Ithute ho khetha pakeng tsa valial vaiali ea valia, ho thellisa le ho thellisa caal ho sengoloa sena!CRIMP Valia Vs. Viap Viss vs. cal cas e vael, ho khetha joang?

Mohlala o nepahetseng oa mohlala o sebetsang:

Kamohelo e loketseng ea mohlala e bohlokoa e bohlokoa bakeng sa ho fokotsa tlhahiso ea likarolo tsa li-confotographic tsa chromatographic. Sena se kenyelletsa ho qoba ho thothomela kapa ho ferekana ha nako ea mohlala nakong ea ho kopanya ka thata ho ka etsa hore likaroloana li hanoe ka sejaneng kapa mohlala. Ts'ebeliso ea Lisebelisoa tse Hloekileng, Insket tse kang lipeipi tsa khalase le li-vaiss li tla thusa ho boloka litlolo le ho fokotsa monyetla oa tšilafalo. Ntle le moo, boloka mehlala tikolohong e laoloang e se nang lerole le likaroloana tse ling tsa sefofane li fokotsa kotsi ea li-blockhu.

Tlhahlobo ea vaul:

Pele o sebelisaLithako tsa Chromatograft, tlhatlhobo e bonoang e bohlokoa ho bona matšoao a bonahalang a ho silafatsa litšila kapa taba ea lihlahisoa. Lekola e le ntho e tloaelehileng tlasa maemo a loketseng a mabone a ho tseba likarolo, litsiti kapa majalefa a mang. Li-viols tse bonts'ang matšoao a tšilafalo li lokela ho lahloa kapa tse hloekisitsoeng pele li sebelisoa. Li-pielis tse pepeneneng tse tsamaisang tlhahlobo le ho netefatsa hore likhama tse hloekileng le tse sa sebetseng li sebelisetsoa ho boloka mohlala le ho hlahlojoa.

Ts'ebeliso ea Liphalo tsa Bohlokoa Boleng:

Ho fokotsa mathata a khofalang, ho bohlokoa haholo ho khetha likhama tsa boemo bo holimo tsa charomagraphy. Liphate tse entsoeng ka thepa ea Premium e joalo ka likhalase tse bohrong li fana ka likhatiso tse ntle tsa lik'hemik'hale le phetoho e tlase, ho fokotsa sampole. Ntle le moo, li-pillymer tsa polyster tse entsoeng ka thepa e kang polypropylene kapa PTFE li loketse likopo tse itseng tse hlokang likopo tse itseng le tse sa sebetseng. Ho khetha lintho tse entsoeng ka li-vial tse netefalitsoeng ka mokhoa oa thuto ea mahlaseli ha ho etsa hore ts'ebetso e tšepahalang le botšepehi ba mohlala.

Tlhokomelo ea tsamaiso ea kamehla:

Ho thibela ho koala le ho netefatsa ts'ebetso e nepahetseng, ho bohlokoa ho kenya tšebetsong kemiso e phethahetseng ea tokiso bakeng sa sistimi ea hau ea choromatography. Ho hloekisa le ho hlaphoheloa likotlo tse tahang khafetsa, ho itlama ho tlosa likaroloana tse bokelletsoeng le masala a ka bakang li-blockrages. Sebelisa baemeli le mekhoa e lumellanang ea ho hloekisa le lits'ebetso tse khothalelitsoeng ke moetsi oa lisebelisoa ho qoba ho senya le tšilafalo. Liteko tsa tsamaiso ea khafetsa, tse kang liteko tsa khatello le ho bolaoa ha khatello, ho tla etsa hore ho be le matla a ts'ebetso le tšebeletso ea litšebeletso.

Lekola li-hplc tse tloaelehileng tsa Hplc le Septa ka botlalo sehloohong sena! : Bakeng sa li-cap tsa vplc valc le Septa, o hloka ho tseba

Tlhahlobo ea boholo ba eona:

Ho etsa tlhahlobo ea boholo ba mohlala ea mohlala ho na le leseli la bohlokoa mabapi le sebopeho le ho tsamaisa lihlahisoa. Mekhoa e kang ea laser e joalo ka laser le microscopy e ka sebelisoa ho tšoaea phetisetso ea boholo le ho supa boholo ba likarolo tse kholo le mehloli e ka bang teng ea tšilafalo. Tlhahisoleseling ena e thusa ha ho khethoa mokhoa oa ho khetha mekhoa e loketseng ea ho feto-fetoha le litlhoko tsa sampole, ho fokotsa kotsi ea ho hlahlojoa.

Tšebelisano 'moho le Barekisi:

Ho sebetsa ka barekisi ba vaele le ho ba fono e tsamaisang thepa le lisebelisoa ho sebetsana le mathata a ho koala. Barekisi ba ka khothaletsa liphetoho tse loketseng tsa valia, mefuta ea filthara le melaoana ea tlhokomelo e thehiloeng litlhoko tsa hau tsa tlhahlobo ea laboratoring le litšobotsi tsa sampole. Li kanna tsa fana ka koetliso mabapi le ho boloka ka nepo le polokelo ho ntlafatsa ts'ebetso ea Val le ho fokotsa litšitiso tse amanang le li-blow. Ho theha kamano e kopanetsoeng ho etsa hore tšehetso e tsoelang pele le phihlello e tsoetseng pele ea ho boloka botshepehi ba chromaticgraphic.

Ka ho kenya tšebetsong maabone ana, laboratori a ka a rarolla ka katleho a bakoang ke lintho tse bakoang ke maemo a tengli-chromatographicphicgraphicle ho netefatsa sephetho se tšepahalang le se sa fetoheng. Ntle le mohlala o nepahetseng oa mohlala o sebetsang le tlhokomelo ea sesebelisoa, mehato e ikemetseng ke senotlolo sa ho ntlafatsa ts'ebetso ea chromatographic le boleng ba data.

Fumana likarabo tse 50 mabapi le li-pilc tse phetoang sechabeng sena se akaretsang! Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Lintlha tse mabapi le lintlha tse mabapi le li-chromatography tsa Vialigraphy

E 'ngoe:

Ho sebetsana le tšenyo ea siling ea syringe nakong ea ts'ebeliso