Boholo ba chromatography, motheo oa k'hemistri oa tlhaiso, o itšetleha ka likarolo tse ngata ho ba le litholoana tse nepahetseng le tse tšepahalang. Le hoja hangata e koahetsoe ke likarolo tse ling, karolo ea li-chromatographic cap e bohlokoa haholo. Sengoliloeng sena se ikemiselitse ho fana ka kutloisiso e felletseng ea hore na ke hobaneng ha ke khethacap e nepahetsenge bohlokoa bakeng sa katleho ea ts'ebetso ea chromatographic.

1. Ts'ireletso ea litšila:

Ho bontša litšila ho beha kotsing e kholo ho nepahala ha liphetho tsa sekaseka. Likatse tsa chromatographic li sebetsa e le thebe e sireletsang, ho sireletsa mohlala ho tsoa ho lerōle le kang lerōle le kang lerōle le nang le moea. Ntle le cappo e loketseng, bohloeki ba sampole bo ka sekisoa, e ka fokolisang liphethollo le fokotsa ho tšepahala.

2. Ho tšepahala ha tiiso:

Ho boloka tiiso ea moea e bohlokoa e bohlokoa ho thibela ho tsoa metsoako e sa tsitsang le ho netefatsa botsitso ba mohlala. Litšireletso tse fapaneng tsa chromographic li hloka lisebelisoa le meralo e itseng ea ho fihlela tiiso e sebetsang. Liphetoho tse kang mocheso, khatello le mofuta oa mohlala o tlameha ho nahaneloa ha o khetha acapHo etsa bonnete ba hore botšepehi ba tlhahlobo ea chromographic.

Bula liphapang pakeng tsa li-Crimp tsa bolaoli, li-vial CRIMP Valia Vs. Viap Viss vs. cal cas e vael, ho khetha joang?

3. Kamohelo ea lik'hemik'hale:

Li-solvet tse fapaneng tsa solvent le lialhune tse sebelisitsoeng ho chromatography li hloka li-cap tsa lik'hemik'hale. Ho se lumellane ho ka lebisa karabelo ea lik'hemik'hale, tšilafalo kapa phallo ea lintho tsa cap. Ho utloisisa ka tatellano ea lik'hemik'hale tsa li-caps tse nang le li-solvents tse fapaneng ho bohlokoa bakeng sa tlhahlobo e atlehileng ea Chrotographic.

4. Li-liners le li-stoms:

Linester tsa cap le li-stoms li fana ka tšireletso e eketsehileng bakeng sa likhahla tsa li-chromatographic. Hangata e etsoa ka thepa e kang ptfe kapa silicone, li-li-li-li-has li fana ka tšitiso e eketsehileng khahlanong le tšilafalo le ho thusa ho lula e le tiiso e nepahetseng. Khetho ea thepa ea Septum e bohlokoa ka tsela e tšoanang kaha e ama botšepehi ba sampole. Khetho e hlokolosi ea linester le ha li-booptius li tla netefatsa ts'ebetso e nepahetseng ts'ebetsong ea li-chrotographic.

Qoba ho isa botebong ba 'nete ea li-vaiali tse phahameng tse holimo-fihla lintlha tsohle tseo u li hlokang ka ho hlahloba sengoloa sena se rutang:1.5ml 11mm snap Ring Vialian ND11

5. Ho tsilatsila mocheso:

Tlhahlobo ea li-chromatographic hangata e amahanngoa le mocheso oa mocheso. Caps e fanang ka botsitso holim'a tsela e ngata ea mocheso ha e ea bohlokoa. Likatse li tlameha ho mamella maemo a hohelang a se nang ho sekisetsa botshepehi ba li-chromatograph ho netefatsa hore sephetho se sa khaotseng li-chromatographic, ho totobatsa tlhokahalo ea ts'ebetso ea khetho e ntle.

Qetellong, ha e tlela chromatography, Diabolose o ka botlalo. AKe cap e loketsengBakeng sa li-chromatography tse sa lumellaneng le sena hase tlatsetso feela. Ke karolo ea bohlokoa. Ho tloha the beshileng khahlanong le tiiso ea mohlokomeli, ea nkang karolo ea bohlokoa ho boloka botšepehi ba mohlala mme bo fumana liphetho tse nepahetseng. Bafuputsi le bahlahlobisisi ba tlameha ho etsa hore lintlha tse kang puisano ea lik'hemik'hale le tsitla ha li lekanya li-cap tsa li-chromatographgramographic.

Lekola ka mokhoa o tebileng ho li-caps tsa hplc tse tloaelehileng le Septa ka ho qoela sengoloa sena se felletseng: Bakeng sa li-cap tsa vplc valc le Septa, o hloka ho tseba

E fetileng:

Hobaneng ha ptfe \ silicone SEPTA e sebelisitsoeng Charomatography? Mabaka a 5

E 'ngoe:

Ho boloka lipale tsa ciaromatography hantle ho boloka mehato ea ho boloka lintlha tse 6