Mokhoa oa chrotography, mokhoa oa mantlha oa tlhahlobo, o sebelisoa masimong a fapaneng a saense bakeng sa tlhahlobo e nepahetseng ea saense. Ho nepahala ha li-chrortographic liphetho tsa chrortographic ho latela boleng le botshepehi ba mohlala o bolokiloeng. E 'ngoe ea likarolo tsa bohlokoa ho boloka botšepehi ke ho boloka likhatiso tse nepahetseng tsa li-chromatographic. Khobotaiso ena ea tataiso ea mekhoa ea mantlha ebile e fana ka mokhoa o phethahetseng oa ho boloka boleng ba sampole nakong ea polokelo.

1.Chose VALY e nepahetseng:

Mohato oa pele oa ho boloka botšepehi ba mohlala o kenyelletsa ho khetha tse loketsengCHROMATIGphic. Lisebelisoa tse kang khalase ea borolilicate kapa polasetiki ea innert li khothalletsoa ho fokotsa tšebelisano lipakeng tsa mohlala le sejana. Nahana ka mokhoa o ikhethang oa chromatographic o sebelisitsoeng (GS CHROMATOLES (GC) kapa metsi chromatography (LC). Ho khetha likhama tse nepahetseng ho beha motheo oa sistimi ea polokelo ea polokelo.

Li-vies tsa khalase

Li-vies tsa khalase, Haholo-holo ba entsoeng ka khalase ea borolivate, ba thibeloa haholo lik'hale lik'hilograma 'me ba ka ba thata ho li-dempole, li ba etsa hore ba tšoanelehe bakeng sa tlhahlobo e tebileng. Khalase e khona ho mamella mocheso o phahameng ho feta polasetiki, e ka bang molemo bakeng sa likopo tse itseng tse hlokang ho tsitsa. Li-vial tsa khalase tsa Amber li etselitsoe ho sireletsa mehlala e bobebe ho tloha ho senya tsa UV, ho netefatsa botsitso ba nako e telele.

Li-vials tsa polasetiki

Li-vials tsa polasetiki, e kang tse entsoeng polypropylene, li bobebe ebile li tšoarella ho feta li-viavers tsa khalase. Sena se na le thuso maemong ao ho monyebetseng ho atileng. Ka kakaretso, li-vaels tsa polasetiki ha li theko e boima ho feta litlolo tsa khalase, li li etsa khetho e sebetsang bakeng sa litlhahlobo tsa lik'hemik'hale moo ho hanyetsa ka lik'hemik'hale. Ha polanete e 'ngoe e ka ba le khanyetso e ntle ea lik'hemik'hale, li kanna tsa se ke tsa tšoaneleha bakeng sa mehlala eohle ea sampole kapa e mabifi.

2.cleanination ke senotlolo:

Ho bohlokoa ho netefatsa hore masea le li-cap li hloekile ka mokhoa o phethahetseng pele li boloka mehlala. Ho na le litšila kapa lithibelo tse siiloeng li ka senya botšepehi ba sampole. Hlatsoa likotlo le li-cap tse nang le tharollo e loketseng ea ho hloekisa, e lateloang ke ho hlakoloa e nang le solvent e phahameng ea ho tlosa litšila. Ts'ebetso ea ho hloekisa ka hloko e lokisa sampole e bolokiloeng bakeng sa ho boloka bohloeki ba bona.

U lakatsa ho utloisisa tsebo e felletseng ea ho hloekisa li-cumanography tsa li-chromatography tsa ciaromatography? E qabola sehloohong sa rona sa leseli la maikutlo a theko e phahameng ea mekhoa e atlehang ea ho hloekisa. Notlolla liphiri joale!Ka ho sebetsa hantle! Mekhoa e 5 ea ho hloekisa li-chromatography tsa mohlala oa mohlala

3.Poper tiiso:

Ho boloka tiiso ea moea o matla ho bohlokoa ho thibela ho ikhathatsa moea le mongobo, e ka amang botsitso bo boholo bo itseng. Tiisa limmapa tse sireletsehileng ho theha tiiso ea moea 'me u nahane ka ho sebelisa li-sppit kapa li-liners ho ntlafatsa tiiso. Khetla li-cap tsa lik'hemik'hale ho thibela tšebelisano ka sampole. Mochini o tiisitsoeng ka thata o tiisa boleng ba boleng bo bolelele ba mohlala nakong ea polokelo.

2. Taolo ea 4:

Taolo ea mocheso ke ntho ea bohlokoa molemong oa ho boloka mohlala.Lithako tsa Chromatografte lokela ho bolokoa tikolohong e laoloang ka mocheso (hangata e bata) ho lieha ho etsa liqeto tsa lik'hemik'hale le ho fokotsa kotsi ea ho hlapanya ha mohlala. Bahanyetsi kapa bafetoheli ba loketse mehlala e mengata, empa ho bohlokoa ho tsamaisana le mocheso oa polokelo ho semolao se ikhethang sa sampole e bolokiloeng.

Boloka li-vaiase ka mocheso o sa khaotseng, ka nepo C le 4 ° C le 4 tsa C, ho latela mofuta oa sampole. Qoba liphetoho tse potlakileng ka mocheso oa mocheso ho thibela ho khesoa ha khalase kapa mokhoa o sithabetsang. Hape, netefatsa hore sebaka sa polokelo se omme ho thibela mongobo ho ama botšepehi ba mohlala kapa vae.

5.Protections mabapi le leseli:

Ho pepesetsoa leseli ho ka ba le phello e mpe litumelong, haholomano e sa tsotelleng e bobebe. Nahana ka ho sebelisa amber kapa likhama tse mebala ho sireletsa disampole ho tsoa lebokoseng, haholo haeba li na le metsoako e metsoako e tšoanang. Ho boloka likhama tse lefifi kapa tse opang ho fana ka tšireletso e eketsehileng khahlanong le litšila 'me u netefatse ho tsitsipana le mehlala e metle.

6.Roriganization le ho ngolla:

Ho boloka mokhoa o hlophisehileng oa ho hlophisa lejoeLithako tsa Chromatografte bohlokoa bakeng sa ho khutlisa hantle le ho latellana. Motso o mong le o mong o lokela ho tsejoa ka ho hlaka ka tlhaiso-leseling e nepahetseng joalo ka lebitso la sampole, letsatsi la boitokiso le lintlha tse ling tse loketseng. Sena ha se netefatse feela ba thuisang feela empa se boetse se thusa ho ba le sekamelo se phethahetseng sa sampole e bolokiloeng. Ho kenya tšebetsong mokhatlo o kopaneng le sistimi ea ho ngolisa ka mokhoa o makatsang o tla phallela lithutsoana tsa laboratoring 'me li tlatselletsa ho fihlela liteko tsa mahlale.

Ho boloka ka nepo ha li-chromatographic ke ts'ebetso e mengata e nang le tšusumetso e kholo ho ea botšepehing ea tlhahlobo ea tlhahlobo ea maikutlo. Ka ho latela tataiso e felletseng e boletsoeng tataisong ena, bafuputsi ba ka eketsa ho tšepahala le ho nepahala ha tšoantšiso ea li-chromatographic. Ho qeta nako le tlhokomelo mabapi le mokhoa oa polokelo o sireletsa boleng ba mohlala ebile ho kenya letsoho bakeng sa liteko tsa mahlale le lihlahla.

Bohelehele ka li-pilc vaiation? Lekola likarabo tse 50 tse bohlale tse fetisisang sehloohong sa rona se felletseng. Fumana lintlha tseo u li hlokang bakeng sa kutloisiso e ntle ea litšoantšo tsa HPLC. Hlahloba Joale!Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Ntho e ngoe le e ngoe eo u hlokang ho e tseba ka bohlokoa ba ho khetha cap e nepahetseng bakeng sa li-chromatograph tsa chromatography

E 'ngoe:

Litšobotsi tse 8 tsa Bohlokoa tsa Gtrings tse Vialis tse nang le PTFE COSY Rubber Septa