Seaparo se phahameng sa chromatography (HPTC) ke mokhoa o boima oa tlhahlobo o sebelisoang haholo ho laboratori bakeng sa ho arola, ho tseba le ho beha likarolo tse ngata motsoako. Pelo ea ts'ebetso ena ke Li-pilc tsa hipc, e amanang le likhathatso le mocheso oa matla ho netefatsa litholoana tse nepahetseng le ho boloka botšepehi ba mohlala. Ka blog ena, re tla hlahloba likhatello tse ka hare le tsa kantle tseo Hply li mamellang, hammoho le meeli ea mocheso.

Ho utloisisa li-hiplc tse utloisisang

Li-pilip tsa li-HPLC hangata li etsoa ka khalase ea boleng bo holimo, e tsejoang ka ho tšoarella le ho hanyetsa ho makala a futhumatsang. Mofuta ona oa khalase o tiisa hore likhama tsa litokelo tsa libaka tse laboratori, ho kenyelletsa ho pepesetsoa li-solyts le lik'hemik'hale tse fapaneng. Moralo oa likhama tsena o kenyelletsa likarolo tse kang ascrew capkapa Aluminium cap, e thusang ho boloka tiiso e sireletsehileng nakong ea ho hlahlojoa.

Batla ho tseba likarabo tse 50 mabapi le li-valc vials, ka kopo sheba sengoloa sena: Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

Mamello ea khatello ea kahare

E 'ngoe ea likarolo tse tebileng tsa li-valc vaialis ke bokhoni ba bona ba ho thibela khatello ea ka hare. Nakong ea HPLCC, haholo-holo nakong ea mohlala, likhatello tsa ka hare li ka aha mabaka a kang a soeufatsa kapa khase ea motlakase e tsoang ho lik'hemik'hale.

Ho latela litekanyetso tsa indasteri, li-pilc vaels li ka mamella likhatello tsa kahare tsa ho fihlela ho 500 kpa (ka hoo e ka bang 73 P3) ha u sebelisa ente e le 'ngoe. Ho mamella hona hoa susumetsa ho bohlokoa hobane ho le bohlokoa hobane ho le joalo ho feta moeli ona ho ka lebisa ho siling kapa litiiso tse senyehileng, ho baka tahlehelo ea mohlala kapa tšilafalo ea mohlala.

Na u batla ho tseba haholoanyane ka tekanyetso ea khatello ea li-iziren Headspace le li-cap? Ka kopo bala sengoloa sena ho ithuta ho eketsehileng! Tekanyetso ea khatello ea li-ijiren ke ea li-vielis le li-caps tse kae?

LITLHAKISO TSA BOPHELO

Ha khatello ea kahare ke taba e amehileng, khatello ea ka ntle e phetha karolo ea bohlokoa ts'ebetsong ea li-hipc tse phelisang. Likhatello tsa kantle li ka hlaha linthong tse kang:

Khatello ea kelello: Ho sebetsana le ho tsamaisa mapolanka ho ka ba thusa ka matla a ka ntle a ka sekisang botšepehi ba bona ba 'ona ba' nete.

Maemo a tikoloho: Liphetoho khatello ea tikoloho kapa mocheso o ka ama tsela eo litsonyo li e etsang nakong ea ho hlahlojoa.

Lipapali tsa boleng bo phahameng tsa HPLCli etselitsoe ho hanela likhatello tsa kantle ntle le ho roba. Leha ho le joalo, ho bohlokoa hore basebelisi ba etsetsetse likhang tsena ka hloko le ho li boloka maemong a nepahetseng ho fokotsa likotsi tse amanang le khatello ea maikutlo.

Ho mamella ha mocheso oa li-hilc vaial

Mocheso o bapala karolo ea tšoaetso ea tšoaetso ts'ebetsong ea li-hipl vaianos. Lisebelisoa tse sebelisoang ho etsa lihlahisoa tsena tse Viales li lata meeli ea mocheso oa bona:

Bofesilicate khalase ea khalase ea khalase: Ka kakaretso, e tloaelehilengborolicate khalase ea likhalase tsa khalasee lokela ho bolokoa ka tlase ho likhato tse 100 Celsius (212 degrees Fahrenheit). Haeba mokelikeli ka hare ho letsibolo o lebeletsoe ho pheha, ho bohlokoa ho laola molumo oa kahare oa ho tlatsa karolo ea boraro ea karolo ea boraro ea e leng karolo ea boraro ea Val. Ts'ireletso ena e thusa ho thibela khatello e feteletseng ea khatello ea khatello ea khatello ea khatello ea maikutlo.

Li-vaels tsa aluminium, bakeng sa li-vaiss li na le li-caps tsa aluminium, li ka mamella mocheso o phahameng - ho fihlela likhato tsa 20042 Defree (likhato tsa 392 Celsius (388 degrees Celsius (388 Deversheit). Mamello ena e ntseng e eketseha e etsa hore ba lokele lits'ebetso tse kenyelletsang li-solvents tsa mocheso o phahameng.

Litlamorao Tse Sebetsang Bakeng sa Ts'ebeliso ea Laboratorit

Ho utloisisa khatello le mocheso oa mocheso oa li-hilc tse bohlokoa bakeng sa litsebi tsa laboratori tse itšetlehileng ka lisebelisoa tsena bakeng sa tlhahlobo e nepahetseng. Mona ho na le litlamorao tse sebetsang:

Botšepehi ba mohlala: Ho boloka khatello e nepahetseng le maemo a mocheso le ho tiisa hore sasamople e lula e sa ntsane e sa silafats'oa ebile e sa fetohe ho pholletsa le tlhahlobo ea tlhahlobo.

Ho thibela ho senya: ka ho iphahamisa ho tse khothalelitsoeng khatello ea ka hare le mocheso, basebelisi ba ka fokotsa kotsi ea ho khelosa le litšenyehelo le ho lahleheloa ke mehlala ea bohlokoa.

Ho khetha mefuta e nepahetseng ea masela: Ho khetha mofuta o nepahetseng oa temoso e thehiloeng litlhoko tse itseng tsa tlhahlobo, le lithemparetjha tse lekantsoeng, li ka ntlafatsa ts'ebetso ea lihlahatso.

HPLC Valias e bapala karolo ea bohlokoa ho netefatsa sephetho se nepahetseng sa li-chromatography tse ngata ka ho emisa khatello ea mocheso le kantle ha u ntse u boloka botsitso. Ka ho mamella ka hare ho khatello ea khatello ea maemo a ka bang 500 ka ho bokellana ho latela likhahla tse 100 ka tlase ho likhato tse 200 tsa Canrees Celsius.

Litsebi tsa laboratori li tlameha ho utloisisa lipara tsena ha ho khetha le ho sebetsana le tsona Li-pilc tsa hipc. Ka ho etsa joalo, li ka netefatsa ts'ebetso e nepahetseng, ho thibela tahlehelo ea sampole kapa litšila, 'me li na le sephetho se tšepahalang. Ha theknoloji e ntse e tsoela pele ho li-chromatography, liketsahalo tse ntseng li tsoela pele le moralo oa litsi li tla matlafatsa bokhoni ba bona, ho ba etsa lisebelisoa tse sa sebetseng hantle tsa li-laboratoring tsa sejoale-joale.

E fetileng:

Ho utloisisa GC Septa: Mefuta, e sebelisa le melemo

E 'ngoe:

Li-vavers tse felletseng ke eng?