Li-vavers tse felletseng ke eng?

Li-vavers tse felletseng tsa pholiso, hape tse tsejoangLipalo tse phahameng tsa tsosolosoKapa li-vaverse tsa micro, lijana tsa mohlala tse etselitsoeng ho fokotsa litsamaisoverover le ho holisa mohlala oa ho hlaphoheloa. Ba fihlela sena ka boqapi bo ikhethang ba kahare, hangata ka sethaleng se thoeng kapa se bopehileng se bopehileng se bopehileng se behang metsi ka tlase. Sena se lumella nale ea Autosompampler ho ikopanya le sampole eohle, e fokotsa litšila ebile e ntse e eketseha sensitigraphy.

Batla ho tseba ho eketsehileng ka li-valc tse hlaphohelang tsa hply tse phahameng. Ka kopo sheba sengoloa sena:Lintlha tse phahameng tsa HPLC e phahameng

Likarolo tsa Bohlokoa le Melemo

Meaho e fokolang ea katse: Monyetla o ka sehloohong oa valial e felletseng ke bokhoni ba eona ba ho fokotsa li-minicronity tse 'maloa feela tse sieang likepe tse kholo.

Pholoso ea mohlala ea ho ntlafatsa: Butle-butle e shebileng sampole, e lumella nang le nale ea ho ikhethela metsi, ho eketsa ho hlaphoheloa.

Ntlha e ntlafalitsoeng: Ka ho fokotsa tsieleho ea mohlala, likhahla tse felletseng li ka ntlafatsa boiketlo ba tlhahlobo, haholo ha u sebetsa ka litlhahlobo tse tlase tsa mahloriso.

Kamohelo ea chelete ea AutoSoSopler: Li-vavers tse felletseng li etselitsoe ho lumellana le baetsi ba maemo a tloaelehileng, ho netefatsa ho kenella ha leoatle. Leha ho le joalo, ho ka 'na ha hlokahala hore u fetole boleng ba sefika sa nale.

E loketseng li-sampole tsa bohlokoa: Ha u sebetsa ka mehlala e lekantsoeng kapa e theko e phahameng, li-vavers tse ngata li bohlokoa ho netefatsa hore o fumana data e ngata kamoo ho ka khonehang.

Moralo o le mong kapa o kopantsoeng: Li-vaiali tsa ho hlaphoheloa li kopantsoe le likorale tse kopaneng tse nang le linele tse entsoeng ka khalase ea khalase. Liner li ne li sebelisoa ka tloaelo e bonolo.

Ho khetha chelete e nepahetseng ea ntlafatso e felletseng

Ho khetha manyeletso a lekaneng a felletseng ho latela lintlha tse 'maloa:

Mohlala oa mohlala: Nahana ka molumo o tloaelehileng oa sampole ea hau. Li-vavers tse ling tsa pholiso li ntlafalitsoe bakeng sa mefuta e nyane haholo (e.g., <100 100 μl), ha ba bang ba ka amohela 1,5 ml kapa ho feta). Lijana tsa 10__l li tsepamisa mohopolo tlase ho laesense, netefatsa phepo e phahameng le ha u sebetsa le mehlala ea Trace

Khokahano ea AutoSoSopler: Etsa bonnete ba hore vial e lumellana le mohlala oa hau oa autosampler. Hlahloba boholo ba Via (bophahamo le bophara) le mofuta oa mofuta oa ho koaloa (screw cap, "cap cap, jj.).

Tapiso ea lintho tse bonahalang: Khetha li-viials tse entsoeng ka lisebelisoa tse lumellanang le solvent le sekaseka. Khalase ea borolicate ke khetho e tloaelehileng ka lebaka la botle ba eona ba lik'hemik'hale.

Mofuta oa ho koaloa: Khetha ho koaloa hoa tsitsipana ho thibela mouoane le tšilafalo.PTF \ siliconeke lisebelisoa tse tloaelehileng tsa septum.

Mehopolo e Molemo le Malebela

Phetoho ea bolelele ba nale: Ha o sebelisa li-vavers tse felletseng tsa pholiso, motheo oa motheo

Setifikeiti sa Via: Bakeng sa likopo tse tebileng, nahana ka ho sebelisa lipilisi tse netefalitsoeng tse hlahisitsoeng bakeng sa bohloeki le botumo bo tlase.

Polokelo: Boloka li-vaiss tikolohong e hloekileng, e omileng ho thibela tšilafalo.

Litsenyehelo: Li-vielis tse phahameng tse phahameng li ka theko e boima ho feta li-vaialis tse tloaelehileng

Li-vavers tse felletseng tsa pholisoke sesebelisoa sa boemo ba bohlokoa bakeng sa litsebi tsa linomoro tsa tlhahlobo li sebetsang ka mehlala e fokolang kapa ea bohlokoa. Moralo oa bona o ikhethileng le bokhoni ba bona ba ho fokotsa li etsa hore li be le bohlokoa tsa ho hlasimolla mohlala, ho eketsehileng ke kutloelo-bohloko le ho bolela litholoana tse nepahetseng. Ka hloko ho nahana ka hloko litlhoko tsa hau tse khethehileng le ho khetha mofuta o nepahetseng oa temoso e felletseng, o ka utloisisa bokhoni ba hau bo felletseng mme o fumana leseli la bohlokoa ho sampuls tse nyane.