Lithako tsa ChromatograftKe sesebelisoa sa bohlokoa la laboratoring, ho thusa karolong ea karohano le ho itlhahloba ha metsoako ea thepa e fapaneng. Joalo ka sesebelisoa leha e le sefe sa laboratoring, ka nepo ha tsona e bohlokoa ho netefatsa polokeho ea basebetsi ba laboratoring le ho sireletsa tikoloho. Tataiso ena e fana ka mehato e khethehileng mehatong e tobileng eo lithollo e lokelang ho e lahla livies tse sebelisitsoeng tsa chromatography ka tsela e nang le boikarabelo.

Mabapi le li-vamatography:

Litoro tsa ciaromagraphy e etselitsoe ho tšoara disampole nakong ea lik'hatheke tsa chrotographic. Li fumaneha ka har'a lisebelisoa tse fapaneng, ho kenyelletsa le khalase le polasetiki le ka li-boholo tse fapaneng ho amohela ho amohela mehlala e fapaneng ea mohlala. Botšepehi ba lihlahlobo tsa li-chromagraphic bo itšetlehile haholo ka boleng le bohloeki ba mashano ana. Hang ha tlhahlobo e phethehile, ea ba ea bohlokoa hore ho etsoa hore ho sebelisoe ka botlalo.

Mefuta ea liphatsa tsa chromatography le menahano ea bona ea litšila

Malinyane a ciaromamography a tla ka mefuta e fapaneng, ho kenyelletsa:

Li-vies tsa khalase: Hangata e sebelisoa bakeng sa lits'ebetso tsa hplc le gc. Liphallo tsa khalase li ka ba le li-solvel tse setseng tse hlokang ho lahla ka hloko.

Li-vials tsa polasetiki: E sebelisoa bakeng sa likopo tse khethehileng, likhama tsena li ka ba le maemo a tlase ho feta khalase mme li ka hloka mekhoa e fapaneng ea ho lahla.

Li-vials tse ikhethang: Tsena li kenyelletsa li-vielis tse etselitsoeng likopo tse khethehileng, joalo ka tlhahlobo ea li-suspasis kapa congund (voc).

Mofuta oa vaeele o sebelisoa o tla susumetsa mokhoa oa ho lahla, haholo-holo mabapi le lisebelisoa le litaba tse setseng.

Mehato e 7 ea ho lahla ka nepo:

Mohato 1. UMety "

Ho bohlokoa ho netefatsa hore sampole leha e le ofe ea setseng a chromatography ka ho felletseng e feptjoa ka botlalo pele a lahla. Mohato ona ha o thibele ho tšoaea liphoso, empa hape o tiisa ts'ebetso ea ts'ebetso e latelang ea litšila tse latelang.

Mohato oa 2.Ho qhekella

Li-vielis tse nang le lisebelisoa tse kotsi kapa tse nang le chefo li hloka ts'ebetso e phethahetseng ea ho qhibiliha. Sena se kenyelletsa ho hlatsoa vae le tharollo e loketseng kapa tharollo ea ho hloekisa ea ho tlosa lipampiri tsa sampole kapa li fokotsa kotsi ea litaba tsa tikoloho kapa tsa polokeho.

Mohato oa 3.Karohano

Karohano e nepahetseng ea litšila e bohlokoa bakeng sa taolo e sebetsang. Litoro tsa ciaromagraphy li lokela ho aroloa litšila tse ling tsa laboratori ho phallela ts'ebetso ea ho lahla le ho latela protocol ea taolo ea litšila.

Ke kopa ho sebetsana le tsebo ea ho hloekisa li-cumanography tsa li-cumanotography tsa ciaromatography? Qoba ho sengoloa sena bakeng sa tataiso e felletseng!Ka ho sebetsa hantle! Mekhoa e 5 ea ho hloekisa li-chromatography tsa mohlala oa mohlala

Mohato 4.Ho nchafatsa

Nako le nako ha ho khonahala, Li-Laboratori li lokela ho etsa pele ho ho nchafatsa likotlo tsa chromagraphy.Li-vies tsa khalaseKa ho khetheha hangata e fumaneha hape, empa ho bohlokoa ho lekola lisebelisoa tsa ho nchafatsa tsa lehae bakeng sa litlhoko tsa bona tse itseng. Li-vaes li lokela ho hlatsuoa ka botlalo pele u nyenyefatsa ho kopana le maemo a bohloeki.

Mohato oa 5.Melao ea ho lahla litšila

Ho latela melao ea lehae, mmuso le ea mmuso o tsamaisang litlhapi tsa litšila tsa laboratori ke ho pakela. Litšeno tse ling li na le tataiso e ikhethang bakeng sa ho lahla litšila tse kotsi, 'me laeboratori li lokela ho lula hona joale ho qoba litlamorao tsa molao. Ho latela melao ena ho tla khothaletsa mokhoa o sireletsehileng le o nang le tikoloho.

Mohato oa 6.Buisana le setsebi sa lits'ebeletso sa litšila

Haeba ho hloka botsitso ho hlaha ka mekhoa e nepahetseng ea ho lahla kapa haeba likhama tse kotsi li na le lisebelisoa tse kotsi, ho khothalletsoa hore litsebi tsa taolo ea litšila li hlahlojoe. Litsebi tsena li ka fana ka tataiso ea bohlokoa ka mekhoa e khethehileng ea ho lahla le ho netefatsa hore laboratori ea hau e tsamaisana le melao ea polokeho.

Mohato oa 7.Ho kenella

Maemong a mang, ho theoha ho ka ba mokhoa o loketseng ho talio ea li-corbatography, haholo-holo tse silafetseng le lisebelisoa tse kotsi. Leha ho le joalo, ho bohlokoa ho latela litlaleho tse thehiloeng 'me u batla tataiso ho litsebi tsa lits'ebetso tsa litšila ho netefatsa litlamorao tse mpe tsa tikoloho.

Ho Laola Lintho Tse IkhethilengLithako tsa ChromatograftHa se tlhojoe feela ea ts'ebetso, empa hape le boikarabello boo latsang ba nang le botsitso ba polokeho le tikoloho. Ka ho latela mekhoa e thathamisitsoeng le ho ea ka ho tsebisoa ka melao ea lehae, laboratories e ka kenya letsoho liketsong tse mpe tsa taolo e tsitsitseng. Ho boloka litekanyetso tsa boeletsi ba ikarabellang tsa theboratori li lula li le pelehila pele ho polokeho le ho latela ha ho sebetsana le litakatso tsa li-chromani tsa chromagraphy.

Bohelehele ka li-pilc vaiation? Hlahloba likarabo tse 50 sengolong sena se bolelang. E hlahlobe joale!Boholo ba 50 bo batloang khafetsa ho li-valc tse phehang

E fetileng:

Malinyane a chromotography bakeng sa sampling ea tikoloho le tlhahlobo

E 'ngoe:

Hobaneng ha ptfe \ silicone SEPTA e sebelisitsoeng Charomatography? Mabaka a 5