Disampole tse ling li ntse li ke ke tsa kenngoa ka kotloloho ka mor'a ho itlhahisa. Ho tsitsipana hoa hlokahala ho etsa metsoako ntle le hore o ananele kapa o foka ka li-uv tsa uV kapa li fefolehang.

E Ntlafatsa Sensitivity
E ntlafatsa karohano (phetoho ea boleng)

Lintlha tse ka khonehang li kenyelletsa tahlehelo ea mohlala, tšilafalo, karabelo e sa phethahalang, kapa lihlahisoa tse ngata, tse ka amang ho nepahala kapa ho hlahisa liphoso.

Sampole ea Filtter

Mehlala ea LC e tlameha ho ba yunifomo le lintho tse se nang chelete; Dibaka li ka senya injector kapa thibela kholomo. Mehato e kenyeletsa:

Shake samne pele ho ente 'me u hlahlobe likarolo, turbidity, kapa emulnis
Haeba likaroloana li hlokomeloa, ho theha sampole ho sebelisa membrane e ka bolokang likarolo tsa ≥0.15μm

Litaba tse ka bang teng: mokhoa oa mohlala oa mohlala, tšilafalo ea ho fokotsa karolo ea komporo, le ho batla e fetohelang ho baka liphoso.

Mekhoa ea ho tlosa

Ntlafatso e arola lihlahlobo tse tsoang ha li-gelven kapa tse tlosa lintho tse tloaelehileng (e.g., liprotheine). Li-solvent tsa manyolo e lokela ho ba le chefo e tlase, e nang le litšila, li nyahame, 'me li qhibilitlo tsa mohlala.

Li-so solvet tse tloaelehileng: Dichll acetate, dichloromethane, li-dichloromethane, li-dchloromafor, li-shlorone, Benzene, Bennzene,

Kamora ho ntšoa, sampole e ka kenngoa ka kotloloho kapa ho tsepamisa maikutlo ho eketsa tlhophiso le kutloelo-bohloko.

Mohlala oa mohlala o qholotsang metsi

Mekhatlo ea acid le letsoai: litšila tsa ho ntša litšila le solvent ea manyolo, e ikamahanya le acidic, ebe e ntša kapa e behelle; kapa o ome ka tlasa n₂ 'me a qhalane ka thata e loketseng ka ente.
Metsoako ea mantlha le masenke: litšila tse ntshoang ka solvent ea manyolo, e ikamahanya le mantlha, e ikamahanya le ea mantlha, e ikamahanya le ea mantlha, e ikamahanya le ea mantlha, ebe e ntša kapa e behelletsoe; kapa o ome ka tlasa n₂ 'me a qhalane ka thata e loketseng ka ente.
Likarolo tse sa nke lehlakore: litlolo tse ntshoang ka solvent ea manyolo, ebe li lekola ka kotloloho ke likhatiso tsa chromatography-kholo.

Mohlala oa mohlala o qholotsang mafura

Ho ntša lehare la boqapi kapa ho itima ka kotloloho; kapa o ome ka tlasa n₂ 'me a qhalane ka thata e loketseng ka ente.

Melemo ea ho tšoanela

Mehloli e ka bang teng ea litšila ka tlhahlobo:

Mofuta oa tšoaetso	Mohloli	Mehato ea thibelo
Tikoloho	Khase e kotsi, lerōle, aeros	Boloka kamore ea lisebelisoa e le thoko, e hloekileng; Kenya AC; Laola mongobo <70%; Thibela mongobo, ke lijalo, vibration
Lijana	Khalase, Ceramic, Quartz, Labware ea polasetiki	Khetha lijana tse nepahetseng ho mofuta oa sampole; Hloekileng ka botlalo
Reagents	Liphetoho tsa sehlopha sa chromatography	Sebelisa li-chromatography-Gradeter-Gradeter-Gradeter-Gradeter

Litokisetso tsa thuto e tloaelehileng

Lokisetsa thuto ea kahare ea ka hare pele; Sebelisa sehlopha se le seng maemong le sampole ho fokotsa liphoso
Lintho li tlameha ho ba li-chromatography-linepe le tse tsitsitseng
Boloka tharollo e tloaelehileng ka libotlolong tse sootho ka mocheso o tlase
Bakeng sa livithamini

Mathata a Tloaelehileng a ho PreTleTment le Tharollo

Bothata	Baka	Tharollo
Litlhoro tse sa amaneng ka chromatograph	Litšila	SHAAN FALTER ho sampole ea sampole le ho lekola ente Fetola mofuta oa filthara Sebelisa mekhoa e meng ea ho hloekisa
Karabelo e tlase ea Peak (haholomano e tlase ea mahloriso)	Adsorption on the filthara	Fetola mofuta oa filthara Etsa meralo eohle tlasa maemo a tšoanang Sebelisa mekhoa e meng ea ho hloekisa
Pholoso e tlase	Ho tlosoa	Eketsa nako ea ho tlosa, sebelisa solvent e chesang Fetola mokhoa oa ho hloekisa
Litlhoro tse kenyang, tse khutsufalitsoeng nakong ea kholomo ea kholomo e khutsufalitsoeng	Mohlala oa ho kena-kenana le tšilafalo	Ntlafatsa mokhoa oa ho hloekisa
Ho nepahala ho fosahetseng	Ha e fola	Fokotsa kapa ho khutlisa karohano, karohano, ho tlosoa kapa maemo a mang Sebelisa ho sebetsana le ho ntlafatsa ho ntlafatsa ho nepahala

E fetileng:

E phahameng haholo \ mocheso o tlase haholo oa ho tsitsipana le ho tsitsipalla

E 'ngoe:

Khutlela lenaneng