Lits'oants'o tsa HPLC

HPLC (High Perfect Chromatography Likonteraka tsena li lumella ente e ikhethang ea ho ba masela a manyane, fokotsa litšila, 'me u netefatse hore botšepehi ba motho e tsoetse pele ho nako e ntse e tsoela pele. Sengoliloeng sena se hlahloba mefuta, lisebelisoa, likopo, melemo, melemo le mekhoa e metle eaLits'oants'o tsa HPLC.

U batla ho tseba haholoanyane ka li-intc tse ngata tsa HPLC, ka kopo sheba sengoloa sena:HPTC Vial Intrys: Elisa ho nepahala le ho ba le botshepehi

Li-interts tsa hplc li na le lijana tse nyane tse behiloeng ka hareLipalo tse tloaelehileng tsa HPLCho kenya disampole ka sistimi ea li-chromatography. Ba etsa mesebetsi e mengata ea bohlokoa:

Taba ea ho tsepamisa maikutlo: Lisebelisoa tsa likokonyana li fokotsa bophahamo ba mohlala oa sampole, o lumellang ho tsepamisa maikutlo a mangata. Sena se thusa haholo ha o sebetsa ka mantsoe a lekanyelitsoeng.

Fokotsa tahlehelo ea mehlala: Ka ho fana ka sebaka se senyenyane sa mohlala, kenya thuso ho fokotsa ho lahleheloa ke mohlala oa mohlala oa bohlokoa nakong ea ente.

Thibela litšila: Likokoti li ka thusa ho thibela tšilafalo pakeng tsa li-sampole tse fapaneng, li boloka ho tšepahala ha litholoana tsa liteko.

Ho ikamahanya le litsamaiso tsa khatello tse phahameng: Likokoanyana tsena li etselitsoe ho mamella likhatello tse behiloeng ho HPLC li ntse li boloka botšepehi ba tsona ba HPLC.

Li-hplc valiast kenya mefuta ea ho kenya

Ho na le mefuta e mengata ea lits'oants'o tsa Hplc tse telele, e 'ngoe le e' ngoe e tsamaisa litlhoko tse khethehileng tsa tlhahlobo. Likarolo tsa mantlha li kenyelletsa:

1. Khethollong ka thepa

Likhamphani tsa khalase:

Ho tsitsipalla ka lik'hemik'hale: Linyehelo tsa khalase li na le khanyetso e ntle ea lik'hemik'hale ebile u se ke oa sebelisana le li-solvent kapa li etsa hore li lokele likopo tse phahameng tsa bohloeki.

Likopo: hangata e sebelisoa ho tlhahlobo ea merongmamolou le lijo tse hlokang ho nepahala haholo.

2. Clatifice ka litlhaloso tse ka tlase

Litalente tse ka tlase:

E etselitsoe ho tsepamisa mohopolo ka tlase, fokotsa masala 'me u fetele tefo. E loketseng tlhahlobo ea tranece moo lerotholi le leng le le leng.

Li-Insts tse tlase:

Ho bonolo ho hloekisa le ho sebelisa litšeho, empa ho ka tlohela metsi a setseng. E loketse polokelo ea polokelo ea kakaretso le ho sebetsa mesebetsi.

Colical Base le Polymer SELEMO SENTINT:

Li fana ka botsitso bo eketsehileng ho thibela ho ts'oaroa kapa ho tsamaea ka har'a terata, ho ba etsa hore ba tšoanele tlhahlobo e phahameng ea boikhathollo.

3. Karolelano ea bolelele

Kenya hape ka bolelele bo fapaneng ho amohela boholo bo fapaneng:

Bolelele bo tloaelehileng bo kenyelletsa 5x29 mm le 6x29 mm, e lumellanang le mefuta e fapaneng ea autosoplers le likhama.

Ho khetha sets'oants'o se nepahetseng sa HPTC

Ho na le lintlha tse 'maloa tseo u lokelang ho nahana ka tsona ha u khetha ho kenya seretse se nepahetseng sa hplc:

Mohlala oa mohlala: Bakeng sa lipalo tse nyane tsa sampole, kenya ka tlase ho likhatiso tse tlase li khothalletsoa ho holisa ho hlaphoheloa.

Kamohelo ea lik'hemik'hale: Bakeng sa li-sampole tse sebetsang, lisekela tsa khalase lia khethoa, ha li sallahale lilallo tse ngata tse mabifi, mekotla ea polasetiki e ka sebelisoa.

Litlhokahalo tsa kopo: Ho hlahlobisisa ka mokhoa o phahameng ho ka hloka hore lisebelisoa tse be tse hlophiloeng ka ho hlaka.

U batla ho tseba hore na ke khetha tsela e nepahetseng joang bakeng sa tefiso ea ka ea HPLC? Ka kopo sheba sengoloa sena: Ke khetha e nepahetseng joang bakeng sa vaplc ea ka ea HPLC?

Melemo ea ho sebelisa lits'oants'o tsa hplc tse ngata

Ho kenyelletsa likokoanyana tse boima ho ea ho li-chornology tsa hau tsa chromatography li fana ka melemo e mengata:

1. Mohlala oa pokello ea mohlala

Ka ho nyenyefatsa molumo o mofufutso ofubelitsoeng ka har'a seli, se kopantsoeng o boloka mohlala oa bohlokoa, o nyatsang ha o sebetsa ka bongata bo theko e phahameng kapa o fokolang.

2. Ho nepahala ho ntlafalitsoeng

Ke kenya letsoho ho fapana ka ente ea mohlala ka ho fana ka libuka tse lekanang, li hlahisoa ke litholoana tse tšepahalang, tse khothatsang.

3. Khothatso e ntlafalitsoeng

Ho tsepamisa maikutlo litumellano tse nyane ho ntlafatsa meeli ea ho lemoha bakeng sa litlhahlobo tse tlase tsa mahlofatso ea mokhoa oa hau oa chromatograph e akaretsang ea mokhoa oa hau oa chrotograph.

Ts'ebeliso e nepahetseng le Tlhokomelo ea lits'oants'o tsa HPLC

Ho eketsa ts'ebetso le bophelo ba lits'oants'o tsa hau tsa HPlc tse matlafatsang, tšebeliso e nepahetseng le tlhokomelo li bohlokoa:

Ho hloekisoa kamehla ho bohlokoa ho qoba tšilafalo: Lihlekehleke tsa khalase li ka hloeka ka li-solvent tse loketseng tse sa arabele ka khalase. Li-ints tsa polasetiki li lokela ho hlatsoa ka botlalo ho tlosa masala leha e le afe ho tloha tlhahlobong e fetileng.

Ho thibela kenyelletso ea litšitiso: Kamehla u apara liatlana ha u ntse u kenya litlolo. Qoba ho kopana ka ho toba ka kokoanyana kapa sebakeng se ka hare ho sona.

Boloka thepa e sa sebelisoeng tikolohong e hloekileng, e omileng: Sebelisa ho paka tšireletso ho thibela tšenyo kapa tšilafalo pele ho e sebelisa.

Lits'oants'o tsa HPLC ke sesebelisoa sa bohlokoahali ho Great Cormatography e ntlafatsang ho fana ka mohlala le ho nepahala ha. Ka ho utloisisa mebala, lisebelisoa, likopo le melemo, thepa e ka etsa liqeto tse nang le tsebo mabapi le lihlahlobo tse fapaneng tsa chrotographic. Khetho e nepahetseng le tlhokomelo ena e ka nka nako e telele ho ea ho liphetho tse tšepahalang, tikoloho le lits'ebetso tsa lijo. E le liphatlalatso tsa theknoloji, e ntse e tsoela pele ka ho fuputsa ka har'a mohono oa lineser ho tla ntlafatsa katleho ea bona ho tšehetsa thuto ea boleng bo phahameng.

U batla ho tseba ho khetha mofuta o nepahetseng oa likhalase tsa hau tsa chromatography, ka kopo sheba sengoloa sena:Mokhoa oa ho khetha mofuta o nepahetseng oa micro-kenngoeng bakeng sa li-chromatograph tsa hau tsa chromatography